LOCUS       sequence-333934-       12165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2
            expressing cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Filbin MR, Mehta A, Schneider AM, Kays KR, Guess JR, Gentili M, 
            Fenyves BG, Charland NC, Gonye ALK, Gushterova I, Khanna HK, LaSalle
            TJ, Lavin-Parsons KM, Lilley BM, Lodenstein CL, Manakongtreecheep K,
            Margolin JD, McKaig BN, Rojas-Lopez M, Russo BC, Sharma N, Tantivit 
            J, Thomas MF, Gerszten RE, Heimberg GS, Hoover PJ, Lieb DJ, Lin B, 
            Ngo D, Pelka K, Reyes M, Smillie CS, Waghray A, Wood TE, Zajac AS, 
            Jennings LL, Grundberg I, Bhattacharyya RP, Parry BA, Villani AC, 
            Sade-Feldman M, Hacohen N, Goldberg MB
  TITLE     Longitudinal proteomic analysis of severe COVID-19 reveals 
            survival-associated signatures, tissue-specific cell death, and 
            cell-cell interactions.
  JOURNAL   Cell Rep Med. 2021 May 18;2(5):100287. doi: 
            10.1016/j.xcrm.2021.100287. Epub 2021 May 3.
  PUBMED    33969320
REFERENCE   2  (bases 1 to 12165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2021-05-18"; volume: "2(5):100287. doi"; pages: " 
            10.1016/j.xcrm.2021.100287. Epub 2021 May 3"
COMMENT     Sequence Label: pTRIP-SFFV-Hygro-2A-TMRPSS2
FEATURES             Location/Qualifiers
     source          1..12165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1201..1266
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1396..1416
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1841..1975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1878..1897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1932..1951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2143..2776
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2823..2948
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3441..3674
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3859..3903
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4196..4313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4453..4860
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4879..5901
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4879..5901
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5917..5973
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7727..7907
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9435..9454)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9668..9687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9787..9809
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9847..9865)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9933..10037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10038..10106,10107..10898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10256..10275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11069..11657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11558..11577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11811..11828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11898..11918)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(11903..12165,1..67)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12083..12165,1..53)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
       61 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
      121 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
      181 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
      241 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
      301 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
      361 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
      421 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
      481 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
      541 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
      601 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
      661 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
      721 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
      781 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
      841 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
      901 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
      961 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     1021 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     1081 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     1141 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     1201 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     1261 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     1321 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     1381 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     1441 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     1501 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     1561 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     1621 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     1681 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     1741 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     1801 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     1861 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1921 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     1981 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     2041 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     2101 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     2161 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     2221 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     2281 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     2341 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     2401 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     2461 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     2521 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     2581 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2761 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     2821 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     2881 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2941 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     3001 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     3061 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     3121 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     3181 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     3241 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     3301 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     3361 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     3421 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     3481 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     3541 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     3601 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     3661 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     3721 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     3781 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     3841 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3901 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3961 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     4021 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     4081 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     4141 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta
     4201 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     4261 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     4321 attacaggga cagcagagat ccactttggc tgatacgcgt ctgcagcccc gataaaataa
     4381 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     4441 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
     4501 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
     4561 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
     4621 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
     4681 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
     4741 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
     4801 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
     4861 ccgcgggcta gcgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     4921 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     4981 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     5041 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     5101 ccggaagtgc ttgacattgg ggaatttagc gagagcctga cctattgcat ctcccgccgt
     5161 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     5221 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     5281 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     5341 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     5401 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     5461 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     5521 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     5581 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     5641 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     5701 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     5761 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     5821 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     5881 actcgtccga gggcaaagga acgtacggga agcggagcta ctaacttcag cctgctgaag
     5941 caggctggag acgtggagga gaaccctgga cctggatcca tggctttgaa ctcagggtca
     6001 ccaccagcta ttggacctta ctatgaaaac catggatacc aaccggaaaa cccctatccc
     6061 gcacagccca ctgtggtccc cactgtctac gaggtgcatc cggctcagta ctacccgtcc
     6121 cccgtgcccc agtacgcccc gagggtcctg acgcaggctt ccaaccccgt cgtctgcacg
     6181 cagcccaaat ccccatccgg gacagtgtgc acctcaaaga ctaagaaagc actgtgcatc
     6241 accttgaccc tggggacctt cctcgtggga gctgcgctgg ccgctggcct actctggaag
     6301 ttcatgggca gcaagtgctc caactctggg atagagtgcg actcctcagg tacctgcatc
     6361 aacccctcta actggtgtga tggcgtgtca cactgccccg gcggggagga cgagaatcgg
     6421 tgtgttcgcc tctacggacc aaacttcatc cttcaggtgt actcatctca gaggaagtcc
     6481 tggcaccctg tgtgccaaga cgactggaac gagaactacg ggcgggcggc ctgcagggac
     6541 atgggctata agaataattt ttactctagc caaggaatag tggatgacag cggatccacc
     6601 agctttatga aactgaacac aagtgccggc aatgtcgata tctataaaaa actgtaccac
     6661 agtgatgcct gttcttcaaa agcagtggtt tctttacgct gtatagcctg cggggtcaac
     6721 ttgaactcaa gccgccagag caggattgtg ggcggcgaga gcgcgctccc gggggcctgg
     6781 ccctggcagg tcagcctgca cgtccagaac gtccacgtgt gcggaggctc catcatcacc
     6841 cccgagtgga tcgtgacagc cgcccactgc gtggaaaaac ctcttaacaa tccatggcat
     6901 tggacggcat ttgcggggat tttgagacaa tctttcatgt tctatggagc cggataccaa
     6961 gtagaaaaag tgatttctca tccaaattat gactccaaga ccaagaacaa tgacattgcg
     7021 ctgatgaagc tgcagaagcc tctgactttc aacgacctag tgaaaccagt gtgtctgccc
     7081 aacccaggca tgatgctgca gccagaacag ctctgctgga tttccgggtg gggggccacc
     7141 gaggagaaag ggaagacctc agaagtgctg aacgctgcca aggtgcttct cattgagaca
     7201 cagagatgca acagcagata tgtctatgac aacctgatca caccagccat gatctgtgcc
     7261 ggcttcctgc aggggaacgt cgattcttgc cagggtgaca gtggagggcc tctggtcact
     7321 tcgaagaaca atatctggtg gctgataggg gatacaagct ggggttctgg ctgtgccaaa
     7381 gcttacagac caggagtgta cgggaatgtg atggtattca cggactggat ttatcgacaa
     7441 atgagggcag acggctaact cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7501 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7561 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7621 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaaa
     7681 atcgtcgaga gatgctgcat ataagcagct gctttttgct tgtactgggt ctctctggtt
     7741 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7801 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7861 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7921 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7981 ttgacattat aatagattta gcaggaattg aactaggagt ggagcacaca ggcaaagctg
     8041 cagaagtact tggaagaagc caccagagat actcacgatt ctgcacatac ctggctaatc
     8101 ccagatccta aggattacat taagtttact aacatttata taatgattta tagtttaaag
     8161 tataaactta tctaatttac tattctgaca gatattaatt aatcctcaaa tatcataaga
     8221 gatgattact attatcccca tttaacacaa gaggaaactg agagggaaag atgttgaagt
     8281 aattttccca caattacagc atccgttagt tacgactcta tgatcttctg acacaaattc
     8341 catttactcc tcaccctatg actcagtcga atatatcaaa gttatggaca ttatgctaag
     8401 taacaaatta cccttttata tagtaaatac tgagtagatt gagagaagaa attgtttgca
     8461 aacctgaata gcttcaagaa gaagagaagt gaggataaga ataacagttg tcatttaaca
     8521 agttttaaca agtaacttgg ttagaaaggg attcaaatgc ataaagcaag ggataaattt
     8581 ttctggcaac aagactatac aatataacct taaatatgac ttcaaataat tgttggaact
     8641 tgataaaact aattaaatat tattgaagat tatcaatatt ataaatgtaa tttactttta
     8701 aaaagggaac atagaaatgt gtatcattag agtagaaaac aatccttatt atcacaattt
     8761 gtcaaaacaa gtttgttatt aacacaagta gaatactgca ttcaattaag ttgactgcag
     8821 attttgtgtt ttgttaaaat tagaaagaga taacaacaat ttgaattatt gaaagtaaca
     8881 tgtaaatagt tctacatacg ttcttttgac atcttgttca atcattgatc gaagttcttt
     8941 atcttggaag aatttgttcc aaagactctg aaataaggaa aacaatctat tatatagtct
     9001 cacacctttg ttttactttt agtgatttca atttaataat gtaaatggtt aaaatttatt
     9061 cttctctgag atcatttcac attgcagata gaaaacctga gactggggta atttttatta
     9121 aaatctaatt taatctcaga aacacatctt tattctaaca tcaatttttc cagtttgata
     9181 ttatcatata aagtcagcct tcctcatctg caggttccac aacaaaaatc caaccaactg
     9241 tggatcaaaa atattgggaa aaaattaaaa atagcaatac aacaataaaa aaatacaaat
     9301 cagaaaaaca gcacagtata acaactttat ttagcattta caatctatta ggtattataa
     9361 gtaatctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     9421 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     9481 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     9541 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     9601 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     9661 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     9721 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     9781 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     9841 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9901 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11821 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11881 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    11941 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    12001 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    12061 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    12121 gccccatggc tgactaattt tttttattta tgcagaggcc gaggc
//
