LOCUS       sequence-33395-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 EPSM A208S
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     43..63
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        45..63
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    180..314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(217..236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     271..290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
       61 agggaacaaa agctgggtac cgggcccaat gcattggcgc cgcggcgcgc cgcgaattaa
      121 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      301 atcatgtctg gatctacgta atacgactca ctatagttct agactaagac acactggaac
      361 agcggatgct tggggagccc atctgggtga ggaccttgtc aagccactgc aaaggcccat
      421 tcaggtgcag ctcaatccag cagggggtac tggtcacagt ctgtctcctg tactccgctc
      481 cccagccttt gacgaagctc atgcggatgg tgcacattcg ggtcaactgg tagacagcct
      541 caaagccctg gttgaccgac tgggccagga gggcagcgaa ctcctggttg ttgaagatct
      601 tcaggttgca tcctggtggg atcttgcaga cggtggccgg gtgccagcca tagcgctggt
      661 tacagttggg agactggaca aaaatagcgc tgtcactgag gcactctgcg aagacctccc
      721 ctccgatgta gtagagccgc acgcctcttc cgatgtgtct ccgtgtcagc tccactgctg
      781 cattcctgtt gacattggag agcagcccta ggcagaagcg ctccgaattg gaggggtcgg
      841 tgaagccatc ccacaggtca tg
//
