LOCUS       sequence-333963-        7402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmTral-FDF-ADA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Tritschler F, Braun JE, Eulalio A, Truffault V, Izaurralde E, 
            Weichenrieder O
  TITLE     Structural basis for the mutually exclusive anchoring of P body 
            components EDC3 and Tral to the DEAD box protein DDX6/Me31B.
  JOURNAL   Mol Cell. 2009 Mar 13. 33(5):661-8.
  PUBMED    19285948
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Mar 13. 33(5):661-8."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C
FEATURES             Location/Qualifiers
     source          1..7402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        72..2581
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2601..2666
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2691..2717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4724..4765
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4775..4792
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4815..4832)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4865..4999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4889..4908)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4943..4962
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5600..6188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5680..5699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6359..7150,7151..7219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6982..7001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7220..7324)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7392..7402,1..8)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacgaggccc tttcgtcttc aagaattaat tctatattct aaaaacacaa atgatacttc
       61 taaaaaaaaa tcatgaatgg catcaactct gaatcaaatc tttgcagatg cacctacttc
      121 tcatttccac tgtcacatca tttttccaga tctcgctgcc tgttatgtgg cccacaaacc
      181 aagacacgtt ttatggccat taaagctggc tgatcgtcgc caaacaccaa atacataatg
      241 aatatgtaca cattcgagaa agaagcgatc aaagaagcgt cttcgggcgg agtaggagaa
      301 tgcggaggag aaggagaacg agctgatcta gtatctctcc acaatccaat gccaactgac
      361 caactggcca tattcggagc aatttgaagc caatttccat cgcctggcga tcgctccatt
      421 cttggctata tgtttttcac cgttacccgg ggccattttc aaagactcgt cggcaagata
      481 agattgtgtc actcgctgtc tctcttcatt tgtcgaagaa tgctgaggaa tttcgcgatg
      541 acgtcggcga gtattttgaa gaatgagaat aatttgtatt tatacgaaaa tcagttagtg
      601 gaattttcta caaaaacatg ttatctatag ataattttgt tgcaaaatat gttgactatg
      661 acaaagattg tatgtatata cctttaatgt attctcattt tcttatgtat ttataatggc
      721 aatgatgata ctgatgatat tttaagatga tgccagacca aaaggcttga atttctgcgt
      781 cttttgccga acgcagtgca tgtgcaattg ttgttttttg gaatattcaa ttttcggact
      841 gtccgctttg atttcagttt cttggcttat tcaaaaagca aagtaaagcc aaaaaagcga
      901 gatggcaata ccaaatgcgg caaaacggta gtggaaggaa aggggtgcgg ggcagcggaa
      961 ggaagggtgg ggcggggcgt ggcggggtct gtggctgggc gcgacgtcac cgacgttgga
     1021 gccactcctt tgaccatgtg tgcgtgtgtg tattattcgt gtctcgccac tcgccggttg
     1081 tttttttctt tttatgctgc gctctctcta gcgccatctc gcttacgcat gctcaacgca
     1141 ccgcatgttg ccgtttcctt ttatgcgtca ttttggctcg aaataggcaa ttatttaaac
     1201 aaagattagt caacgaaaac gctaaaataa ataagtctac aatatggtta cttattgcca
     1261 tgtgtgtgca gccaacgata gcaacaaaag caacaacaca ggtggctttc cctctttcac
     1321 tttttgtttg caagccgcgt gcgagcaaga cggcacgacc ggcaaacgca attacgctga
     1381 caaagagcag acgaagtttt ggcgaaaaac atcaaggcgc ctgatacgaa tgcatttgca
     1441 ataacaattg cgatatttaa tattgtttat gaagctgttt gacttcaaaa cacacaaaaa
     1501 aaaaaataaa acaaattatt tgaaagagaa ttaggaatcg gacgcttatc gttagggtaa
     1561 caacaagaaa tgcttactga gtcacagcct ctggaaaact gccgcaagcc agagagagag
     1621 agaaaaagag ggagagcagc ttagaccgca tgtgcttgtg tgtgaggcgt ctctctcttc
     1681 gtctctgttg cgcaaacgca tagactgcac tgaaaaaatc gattacctat tttttatgaa
     1741 tgaatatttg cactattact attcaaaact attaagatag caatcacatt caatagccaa
     1801 atactatacc acctgagcga tgcaacgaaa tgatcaattt gagcaaaaat gctgcatatt
     1861 taggacggca tcattataga aatgcttctt gctgtgtact tttctctcgt ctggcagctg
     1921 tttcgccgtt attgttaaaa ccggcttaag ttaggtgtgt tttctacgac tagtgaatgc
     1981 cctactagaa gatgtgtgtt gcacaaaatg tccctggaat aaccaatttg aagtgcagat
     2041 agcagtaaac gtaagctaat atgaatatta tttaactgta atgttttaat atcgctggac
     2101 attactaata aacccactat aaacacatgt acatatgtat gttttggcat acaatgagta
     2161 gttggggaaa aaatgtgtaa aagcaccgtg accatcacag cataaagata accagctgaa
     2221 gtatcgaata tgagtaaccc ccaaattgaa tcacatgccg caactgatag gacccatgga
     2281 agtacactct tcatggcgat atacaagaca cacacaagca cgaacaccca gttgcggagg
     2341 aaattctccg taaatgaaaa cccaatcggc gaacaattca tacccatata tggtaaaagt
     2401 tttgaacgcg acttgagagc ggagagcatt gcggctgata aggttttagc gctaagcggg
     2461 ctttataaaa cgggctgcgg gaccagtttt catatcacta ccgtttgagt tcttgtgctg
     2521 tgtggatact cctcccgaca caaagccgct ccatcagcca gcagtcgtct aatccagaga
     2581 ccccggatcg gggtaccaac atggacgcac aaacacgacg acgtgagcgt cgcgctgaga
     2641 aacaagctca atggaaagct gcaaacggtg gatctcctcc acatatggct tacccatacg
     2701 atgttccaga ttacgctcct ccaagcttga tgagcggggg attaccggag ttgggctcca
     2761 aaatcagcct catttccaag gcggacattc gatacgaggg ccgcctgtac acggtggatc
     2821 cgcaggagtg caccattgcg ctctcgagtg tgcgatcctt cggtacagaa gatcgggaca
     2881 cccagttcca gatagcgccg caatcgcaga tctacgacta catcctcttc cgtggctcgg
     2941 acatcaagga cattcgcgtt gtcaacaacc acacgctgcc gcaccacaat gacccggcga
     3001 tcatgcaggc gcagctccag aacggaccgc cccagatgcc gcagcacttc cccatgccca
     3061 gcgggatgag cggtccgccg cagcagcaac aggtgccgtc gcagcaaccg ccccagatgc
     3121 caagcggtgg aggcagtggt ggtgctggtg gagcaggagc tcctggcggc ggcggacccg
     3181 gctacggcaa tggcaatccg ttcggcaacc tcggcggtcc caatctggcc aacatggtgg
     3241 gcaatgcggg cggatcgctt gcacccggtt ccggtgctcc gggcagcgga cccttcatgc
     3301 acatcggtgg caatcagcag caaccgaagc cccagcagca gaaacagcca aacatgctgg
     3361 ccggtgcctc gcgatcgacg acgcccatta gcctgattgt gagtcccacg gcggagctga
     3421 ctcagcagca gcagctgcac cagcaacaga acgctggcca gggtggcaac ggacgcgatg
     3481 caggacataa gcgccagaac caccagcagc aacagaatca gcagcatcag caacgtggcg
     3541 gacccagcca caacaatatg cagcatcagc agcaacgagg cggcggcagt ggaacggact
     3601 tctataacca gcagcgggat cgtcgggact ccggacgtca aatggacaac aactatagca
     3661 acaactacaa caacaataat aataatcagc gcaatcgcgg cggcggcaac ggaatgcaac
     3721 agcagcagcg aggaggaaac ggcggcagcg gcggcggcgg tggaaacgga ggtggaaaca
     3781 acccggcctg gaacatgcat cgcggcaacc agaactcgaa caacatgatg aacatgcgca
     3841 accgcggcat gggatcccgc ggccccatgc gacccaatca gggctatcgt ccgcagtcgg
     3901 ccaataatca gaacaagccg cggaacaaga tcaagttcga gggcgacgcg gatgcggagc
     3961 aggcaaacaa caagttcgag gaactgcgct cccaactggc caagctcaag gtggccgagg
     4021 atggtgcacc caagccagcc accaatgcaa cggccgccac tgcaactgca accaatgagc
     4081 aggtgggtga gaaggttgaa ggcgttcaca cactgaatgg cgagaccgac aagaaggatg
     4141 attctggcaa cgagaccggc gctggggagc acgagcctga gggggatgat gttgctgtgt
     4201 gctacgacaa gaccaaatcg ttcttcgaca acatctcgtg cgaggctgcc caggatcgca
     4261 gcaggaacaa gaagaacgat tggcgccagg agcgcaagtt gaacacggag accttcggag
     4321 tgtcctccac acgacgtggc agttaccgcg gacgcaacca atactacaac aataatggca
     4381 acggcggcat taactcggga tatggaggag cgccaggcta caacaggaac aattatcgca
     4441 tgggtggcgg cggcggaaac ttccgaaaca ggagcaacaa tcgcaacaac ggcggcggtc
     4501 gtggcggaaa cggaatgcca aacatcacca atggcaacac ggttgctgcg ctgaaggcgg
     4561 ccaacaatgc tgctggccac ggatccaatg ccacggactc cagtgcacca aatgccacaa
     4621 ccgcgacgac aaagtcgacg tccctcttgc cagagcagac gcaacaggtg gcggcagttt
     4681 cacaataggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     4741 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
     4801 aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat
     4861 cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     4921 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     4981 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     5041 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     5101 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     5161 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     5221 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     5281 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     5341 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     5401 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     5461 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     5521 aggtagatga cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa
     5581 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5641 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5701 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5761 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5821 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5881 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5941 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6001 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     6061 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6121 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6181 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6241 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6301 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6361 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6421 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6481 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6541 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6601 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6661 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6721 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6781 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6841 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6901 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6961 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7021 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7141 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7201 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7261 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7321 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     7381 taacctataa aaataggcgt at
//
