LOCUS       sequence-333964-        6335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmTral-LSmR21EE23KS13AI15A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C
FEATURES             Location/Qualifiers
     source          1..6335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..50
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        114..2623
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2643..2645,2646..2648,2649..3359)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2643..2645,2646..2648,2649..3359)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2688..2709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2949..2968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3296..3317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3699..3740
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3750..3767
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3790..3807)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3840..3974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3864..3883)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3918..3937
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4575..5163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4655..4674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5334..6125,6126..6194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5957..5976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6195..6299)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
       61 tcaagaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa aatcatgaat
      121 ggcatcaact ctgaatcaaa tctttgcaga tgcacctact tctcatttcc actgtcacat
      181 catttttcca gatctcgctg cctgttatgt ggcccacaaa ccaagacacg ttttatggcc
      241 attaaagctg gctgatcgtc gccaaacacc aaatacataa tgaatatgta cacattcgag
      301 aaagaagcga tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg agaaggagaa
      361 cgagctgatc tagtatctct ccacaatcca atgccaactg accaactggc catattcgga
      421 gcaatttgaa gccaatttcc atcgcctggc gatcgctcca ttcttggcta tatgtttttc
      481 accgttaccc ggggccattt tcaaagactc gtcggcaaga taagattgtg tcactcgctg
      541 tctctcttca tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc gagtattttg
      601 aagaatgaga ataatttgta tttatacgaa aatcagttag tggaattttc tacaaaaaca
      661 tgttatctat agataatttt gttgcaaaat atgttgacta tgacaaagat tgtatgtata
      721 tacctttaat gtattctcat tttcttatgt atttataatg gcaatgatga tactgatgat
      781 attttaagat gatgccagac caaaaggctt gaatttctgc gtcttttgcc gaacgcagtg
      841 catgtgcaat tgttgttttt tggaatattc aattttcgga ctgtccgctt tgatttcagt
      901 ttcttggctt attcaaaaag caaagtaaag ccaaaaaagc gagatggcaa taccaaatgc
      961 ggcaaaacgg tagtggaagg aaaggggtgc ggggcagcgg aaggaagggt ggggcggggc
     1021 gtggcggggt ctgtggctgg gcgcgacgtc accgacgttg gagccactcc tttgaccatg
     1081 tgtgcgtgtg tgtattattc gtgtctcgcc actcgccggt tgtttttttc tttttatgct
     1141 gcgctctctc tagcgccatc tcgcttacgc atgctcaacg caccgcatgt tgccgtttcc
     1201 ttttatgcgt cattttggct cgaaataggc aattatttaa acaaagatta gtcaacgaaa
     1261 acgctaaaat aaataagtct acaatatggt tacttattgc catgtgtgtg cagccaacga
     1321 tagcaacaaa agcaacaaca caggtggctt tccctctttc actttttgtt tgcaagccgc
     1381 gtgcgagcaa gacggcacga ccggcaaacg caattacgct gacaaagagc agacgaagtt
     1441 ttggcgaaaa acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt
     1501 aatattgttt atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta
     1561 tttgaaagag aattaggaat cggacgctta tcgttagggt aacaacaaga aatgcttact
     1621 gagtcacagc ctctggaaaa ctgccgcaag ccagagagag agagaaaaag agggagagca
     1681 gcttagaccg catgtgcttg tgtgtgaggc gtctctctct tcgtctctgt tgcgcaaacg
     1741 catagactgc actgaaaaaa tcgattacct attttttatg aatgaatatt tgcactatta
     1801 ctattcaaaa ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc
     1861 gatgcaacga aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata
     1921 gaaatgcttc ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa
     1981 aaccggctta agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg
     2041 ttgcacaaaa tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta
     2101 atatgaatat tatttaactg taatgtttta atatcgctgg acattactaa taaacccact
     2161 ataaacacat gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt
     2221 aaaagcaccg tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac
     2281 ccccaaattg aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg
     2341 atatacaaga cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa
     2401 aacccaatcg gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga
     2461 gcggagagca ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc
     2521 gggaccagtt ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga
     2581 cacaaagccg ctccatcagc cagcagtcgt ctaatccaga gaccccggat cggggtacca
     2641 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2701 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2761 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2821 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2881 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2941 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3001 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3061 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3121 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3181 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3241 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     3301 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc
     3361 caagcttgaa ttctatgagc gggggattac cggagttggg ctccaaaatc gcgctcgcgt
     3421 ccaaggcgga cattgagtac aagggccgcc tgtacacggt ggatccgcag gagtgcacca
     3481 ttgcgctctc gagtgtgcga tccttcggta cagaagatcg ggacacccag ttccagatag
     3541 cgccgcaatc gcagatctac gactacatcc tcttccgtgg ctcggacatc aaggacattc
     3601 gcgttgtcaa caaccacacg ctgccgcacc acaatgaccc ggcgatcatg caggcgcagc
     3661 tcggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     3721 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     3781 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     3841 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3901 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3961 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     4021 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     4081 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     4141 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     4201 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     4261 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     4321 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     4381 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     4441 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     4501 gatgacgacc atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4981 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     5041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5641 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5941 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6301 acatttcccc gaaaagtgcc acctgacgtc taaga
//
