LOCUS       sequence-333965-        8004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmTral-R42EF44A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmTral-R42EF44A_C
FEATURES             Location/Qualifiers
     source          1..8004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..120
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        183..2692
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2712..2714,2715..2717,2718..3428)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2712..2714,2715..2717,2718..3428)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3018..3037)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3365..3386
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5438..5479
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5489..5506
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5529..5546)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5579..5713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5603..5622)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5657..5676
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6314..6902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6394..6413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7073..7864,7865..7933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLMDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7696..7715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(7934..8004,1..34))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
       61 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      121 cctttcgtct tcaagaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa
      181 atcatgaatg gcatcaactc tgaatcaaat ctttgcagat gcacctactt ctcatttcca
      241 ctgtcacatc atttttccag atctcgctgc ctgttatgtg gcccacaaac caagacacgt
      301 tttatggcca ttaaagctgg ctgatcgtcg ccaaacacca aatacataat gaatatgtac
      361 acattcgaga aagaagcgat caaagaagcg tcttcgggcg gagtaggaga atgcggagga
      421 gaaggagaac gagctgatct agtatctctc cacaatccaa tgccaactga ccaactggcc
      481 atattcggag caatttgaag ccaatttcca tcgcctggcg atcgctccat tcttggctat
      541 atgtttttca ccgttacccg gggccatttt caaagactcg tcggcaagat aagattgtgt
      601 cactcgctgt ctctcttcat ttgtcgaaga atgctgagga atttcgcgat gacgtcggcg
      661 agtattttga agaatgagaa taatttgtat ttatacgaaa atcagttagt ggaattttct
      721 acaaaaacat gttatctata gataattttg ttgcaaaata tgttgactat gacaaagatt
      781 gtatgtatat acctttaatg tattctcatt ttcttatgta tttataatgg caatgatgat
      841 actgatgata ttttaagatg atgccagacc aaaaggcttg aatttctgcg tcttttgccg
      901 aacgcagtgc atgtgcaatt gttgtttttt ggaatattca attttcggac tgtccgcttt
      961 gatttcagtt tcttggctta ttcaaaaagc aaagtaaagc caaaaaagcg agatggcaat
     1021 accaaatgcg gcaaaacggt agtggaagga aaggggtgcg gggcagcgga aggaagggtg
     1081 gggcggggcg tggcggggtc tgtggctggg cgcgacgtca ccgacgttgg agccactcct
     1141 ttgaccatgt gtgcgtgtgt gtattattcg tgtctcgcca ctcgccggtt gtttttttct
     1201 ttttatgctg cgctctctct agcgccatct cgcttacgca tgctcaacgc accgcatgtt
     1261 gccgtttcct tttatgcgtc attttggctc gaaataggca attatttaaa caaagattag
     1321 tcaacgaaaa cgctaaaata aataagtcta caatatggtt acttattgcc atgtgtgtgc
     1381 agccaacgat agcaacaaaa gcaacaacac aggtggcttt ccctctttca ctttttgttt
     1441 gcaagccgcg tgcgagcaag acggcacgac cggcaaacgc aattacgctg acaaagagca
     1501 gacgaagttt tggcgaaaaa catcaaggcg cctgatacga atgcatttgc aataacaatt
     1561 gcgatattta atattgttta tgaagctgtt tgacttcaaa acacacaaaa aaaaaaataa
     1621 aacaaattat ttgaaagaga attaggaatc ggacgcttat cgttagggta acaacaagaa
     1681 atgcttactg agtcacagcc tctggaaaac tgccgcaagc cagagagaga gagaaaaaga
     1741 gggagagcag cttagaccgc atgtgcttgt gtgtgaggcg tctctctctt cgtctctgtt
     1801 gcgcaaacgc atagactgca ctgaaaaaat cgattaccta ttttttatga atgaatattt
     1861 gcactattac tattcaaaac tattaagata gcaatcacat tcaatagcca aatactatac
     1921 cacctgagcg atgcaacgaa atgatcaatt tgagcaaaaa tgctgcatat ttaggacggc
     1981 atcattatag aaatgcttct tgctgtgtac ttttctctcg tctggcagct gtttcgccgt
     2041 tattgttaaa accggcttaa gttaggtgtg ttttctacga ctagtgaatg ccctactaga
     2101 agatgtgtgt tgcacaaaat gtccctggaa taaccaattt gaagtgcaga tagcagtaaa
     2161 cgtaagctaa tatgaatatt atttaactgt aatgttttaa tatcgctgga cattactaat
     2221 aaacccacta taaacacatg tacatatgta tgttttggca tacaatgagt agttggggaa
     2281 aaaatgtgta aaagcaccgt gaccatcaca gcataaagat aaccagctga agtatcgaat
     2341 atgagtaacc cccaaattga atcacatgcc gcaactgata ggacccatgg aagtacactc
     2401 ttcatggcga tatacaagac acacacaagc acgaacaccc agttgcggag gaaattctcc
     2461 gtaaatgaaa acccaatcgg cgaacaattc atacccatat atggtaaaag ttttgaacgc
     2521 gacttgagag cggagagcat tgcggctgat aaggttttag cgctaagcgg gctttataaa
     2581 acgggctgcg ggaccagttt tcatatcact accgtttgag ttcttgtgct gtgtggatac
     2641 tcctcccgac acaaagccgc tccatcagcc agcagtcgtc taatccagag accccggatc
     2701 ggggtaccat catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2761 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2821 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2881 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     2941 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3001 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3061 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3121 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3181 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3241 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3301 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3361 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3421 tgtacaagcc aagcttgaat tctatgagcg ggggattacc ggagttgggc tccaaaatca
     3481 gcctcatttc caaggcggac attcgatacg agggccgcct gtacacggtg gatccgcagg
     3541 agtgcaccat tgcgctctcg agtgtggagt ccgcgggtac agaagatcgg gacacccagt
     3601 tccagatagc gccgcaatcg cagatctacg actacatcct cttccgtggc tcggacatca
     3661 aggacattcg cgttgtcaac aaccacacgc tgccgcacca caatgacccg gcgatcatgc
     3721 aggcgcagct ccagaacgga ccgccccaga tgccgcagca cttccccatg cccagcggga
     3781 tgagcggtcc gccgcagcag caacaggtgc cgtcgcagca accgccccag atgccaagcg
     3841 gtggaggcag tggtggtgct ggtggagcag gagctcctgg cggcggcgga cccggctacg
     3901 gcaatggcaa tccgttcggc aacctcggcg gtcccaatct ggccaacatg gtgggcaatg
     3961 cgggcggatc gcttgcaccc ggttccggtg ctccgggcag cggacccttc atgcacatcg
     4021 gtggcaatca gcagcaaccg aagccccagc agcagaaaca gccaaacatg ctggccggtg
     4081 cctcgcgatc gacgacgccc attagcctga ttgtgagtcc cacggcggag ctgactcagc
     4141 agcagcagct gcaccagcaa cagaacgctg gccagggtgg caacggacgc gatgcaggac
     4201 ataagcgcca gaaccaccag cagcaacaga atcagcagca tcagcaacgt ggcggaccca
     4261 gccacaacaa tatgcagcat cagcagcaac gaggcggcgg cagtggaacg gacttctata
     4321 accagcagcg ggatcgtcgg gactccggac gtcaaatgga caacaactat agcaacaact
     4381 acaacaacaa taataataat cagcgcaatc gcggcggcgg caacggaatg caacagcagc
     4441 agcgaggagg aaacggcggc agcggcggcg gcggtggaaa cggaggtgga aacaacccgg
     4501 cctggaacat gcatcgcggc aaccagaact cgaacaacat gatgaacatg cgcaaccgcg
     4561 gcatgggatc ccgcggcccc atgcgaccca atcagggcta tcgtccgcag tcggccaata
     4621 atcagaacaa gccgcggaac aagatcaagt tcgagggcga cttcgatttc gagcaggcaa
     4681 acaacaagtt cgaggaactg cgctcccaac tggccaagct caaggtggcc gaggatggtg
     4741 cacccaagcc agccaccaat gcaacggccg ccactgcaac tgcaaccaat gagcaggtgg
     4801 gtgagaaggt tgaaggcgtt cacacactga atggcgagac cgacaagaag gatgattctg
     4861 gcaacgagac cggcgctgga gagcacgagc ctgaggagga tgatgttgct gtgtgctacg
     4921 acaagaccaa atcgttcttc gacaacatct cgtgcgaggc tgcccaggat cgcagcaaga
     4981 acaagaagaa cgattggcgc caggagcgca agttgaacac ggagaccttc ggagtgtcct
     5041 ccacacgacg tggcagttac cgcggacgca accaatacta caacaataat ggcaacggcg
     5101 gcattaactc gggatatgga ggagcgccag gctacaacag gaacaattat cgcatgggtg
     5161 gcggcggcgg aaacttccga aacaggagca acaatcgcaa caacggcggc ggtcgtggcg
     5221 gaaacggaat gccaaacatc accaatggca acacggctgc tgcgctgaag gcggccaaca
     5281 atgctgctgg ccacggatcc aatgccacgg actccagtgc accaaatgcc acaaccgcga
     5341 cgacaaagtc gacgtccctc ttgccagagc agacgcaaca ggtggcggca gtttcacaat
     5401 aggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     5461 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
     5521 ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga
     5581 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     5641 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     5701 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     5761 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
     5821 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     5881 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     5941 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     6001 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     6061 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     6121 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     6181 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     6241 atgacgacca tcagggacag cttcaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6721 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7381 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7561 tggcagcact gcataattct cttactgtca tgccatccat aagatgcttt tctgtgactg
     7621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7681 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7981 tcatgagcgg atacatattt gaat
//
