LOCUS       sequence-333969-        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmTral-LSmR21EE23KR42EF44A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: 
            pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..37
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        101..2610
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2630..2695
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2720..2746
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3089..3130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3140..3157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3180..3197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3230..3364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3254..3273)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3308..3327
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3965..4553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4045..4064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4724..5515,5516..5584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5347..5366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5585..5689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaatt
       61 ctatattcta aaaacacaaa tgatacttct aaaaaaaaat catgaatggc atcaactctg
      121 aatcaaatct ttgcagatgc acctacttct catttccact gtcacatcat ttttccagat
      181 ctcgctgcct gttatgtggc ccacaaacca agacacgttt tatggccatt aaagctggct
      241 gatcgtcgcc aaacaccaaa tacataatga atatgtacac attcgagaaa gaagcgatca
      301 aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga aggagaacga gctgatctag
      361 tatctctcca caatccaatg ccaactgacc aactggccat attcggagca atttgaagcc
      421 aatttccatc gcctggcgat cgctccattc ttggctatat gtttttcacc gttacccggg
      481 gccattttca aagactcgtc ggcaagataa gattgtgtca ctcgctgtct ctcttcattt
      541 gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag aatgagaata
      601 atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt tatctataga
      661 taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac ctttaatgta
      721 ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt ttaagatgat
      781 gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa cgcagtgcat gtgcaattgt
      841 tgttttttgg aatattcaat tttcggactg tccgctttga tttcagtttc ttggcttatt
      901 caaaaagcaa agtaaagcca aaaaagcgag atggcaatac caaatgcggc aaaacggtag
      961 tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg gcggggcgtg gcggggtctg
     1021 tggctgggcg cgacgtcacc gacgttggag ccactccttt gaccatgtgt gcgtgtgtgt
     1081 attattcgtg tctcgccact cgccggttgt ttttttcttt ttatgctgcg ctctctctag
     1141 cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc cgtttccttt tatgcgtcat
     1201 tttggctcga aataggcaat tatttaaaca aagattagtc aacgaaaacg ctaaaataaa
     1261 taagtctaca atatggttac ttattgccat gtgtgtgcag ccaacgatag caacaaaagc
     1321 aacaacacag gtggctttcc ctctttcact ttttgtttgc aagccgcgtg cgagcaagac
     1381 ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga cgaagttttg gcgaaaaaca
     1441 tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc gatatttaat attgtttatg
     1501 aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa caaattattt gaaagagaat
     1561 taggaatcgg acgcttatcg ttagggtaac aacaagaaat gcttactgag tcacagcctc
     1621 tggaaaactg ccgcaagcca gagagagaga gaaaaagagg gagagcagct tagaccgcat
     1681 gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc gcaaacgcat agactgcact
     1741 gaaaaaatcg attacctatt ttttatgaat gaatatttgc actattacta ttcaaaacta
     1801 ttaagatagc aatcacattc aatagccaaa tactatacca cctgagcgat gcaacgaaat
     1861 gatcaatttg agcaaaaatg ctgcatattt aggacggcat cattatagaa atgcttcttg
     1921 ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta ttgttaaaac cggcttaagt
     1981 taggtgtgtt ttctacgact agtgaatgcc ctactagaag atgtgtgttg cacaaaatgt
     2041 ccctggaata accaatttga agtgcagata gcagtaaacg taagctaata tgaatattat
     2101 ttaactgtaa tgttttaata tcgctggaca ttactaataa acccactata aacacatgta
     2161 catatgtatg ttttggcata caatgagtag ttggggaaaa aatgtgtaaa agcaccgtga
     2221 ccatcacagc ataaagataa ccagctgaag tatcgaatat gagtaacccc caaattgaat
     2281 cacatgccgc aactgatagg acccatggaa gtacactctt catggcgata tacaagacac
     2341 acacaagcac gaacacccag ttgcggagga aattctccgt aaatgaaaac ccaatcggcg
     2401 aacaattcat acccatatat ggtaaaagtt ttgaacgcga cttgagagcg gagagcattg
     2461 cggctgataa ggttttagcg ctaagcgggc tttataaaac gggctgcggg accagttttc
     2521 atatcactac cgtttgagtt cttgtgctgt gtggatactc ctcccgacac aaagccgctc
     2581 catcagccag cagtcgtcta atccagagac cccggatcgg ggtaccaaca tggacgcaca
     2641 aacacgacga cgtgagcgtc gcgctgagaa acaagctcaa tggaaagctg caaacggtgg
     2701 atctcctcca catatggctt acccatacga tgttccagat tacgctcctc caagcttgaa
     2761 ttctatgagc gggggattac cggagttggg ctccaaaatc agcctcattt ccaaggcgga
     2821 cattgagtac aagggccgcc tgtacacggt ggatccgcag gagtgcacca ttgcgctctc
     2881 gagtgtggag tccgcgggta cagaagatcg ggacacccag ttccagatag cgccgcaatc
     2941 gcagatctac gactacatcc tcttccgtgg ctcggacatc aaggacattc gcgttgtcaa
     3001 caaccacacg ctgccgcacc acaatgaccc ggcgatcatg caggcgcagc tcggcggccg
     3061 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     3121 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     3181 ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat
     3241 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3301 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3361 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3421 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgaccga
     3481 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     3541 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     3601 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     3661 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     3721 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     3781 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     3841 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     3901 atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     4441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     5041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5341 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5701 gaaaagtgcc acctgacgtc taagaaacca ttattatc
//
