LOCUS       sequence-33398-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 S208A
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     50..67
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 catgctgttt gggaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
       61 actatagaat acaagctact tgttcttttt gcaggatccc atcgattcga attcaccatg
      121 gactacaagg acgacgatga caagtcgtcc atcctgcctt tcactccccc gatcgtgaag
      181 cgcctgctgg gctggaagaa gggcgagcag aacgggcagg aggagaaatg gtgcgagaag
      241 gcggtcaaga gcctggtcaa gaaactcaag aagacggggc agctggacga gctggagaag
      301 gccatcacca cgcagaacgt caacaccaag tgcatcacca tccccaggtc cctggatggc
      361 cggttgcagg tgtcccatcg gaaggggctc cctcatgtca tctactgccg cctgtggcga
      421 tggccagacc tgcacagcca ccacgagctg cgggccatgg agctgtgtga gttcgccttc
      481 aatatgaaga aggacgaggt ctgcgtgaat ccctaccact accagagagt agagacacca
      541 gttctacctc ctgtgttggt gccacgccac acagagatcc cggccgagtt ccccccactg
      601 gacgactaca gccattccat ccccgaaaac actaacttcc ccgcaggcat cgagccccag
      661 agcaatattc cagagacccc accccctggc tacctgagtg aagatggaga aaccagtgac
      721 caccagatga accacagcat ggacgcaggt tctccaaacc tagccccgaa tccgatgtcc
      781 ccagcacata ataacttgga cctgcagcca gttacctact gcgagccggc cttctggtgc
      841 tccatctcct actacgagct gaacacgcgt cgggaa
//
