LOCUS       sequence-333981-       11595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculoviral transfer vector to co-express nsp12-His6-3xFlag and 
            nsp7-Linker-nsp8 fusion in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Bertolin AP, Weissmann F, Zeng J, Posse V, Milligan JC, Canal B, 
            Ulferts R, Wu M, Drury LS, Howell M, Beale R, Diffley JFX
  TITLE     Identifying SARS-CoV-2 antiviral compounds by screening for small 
            molecule inhibitors of nsp12/7/8 RNA-dependent RNA polymerase.
  JOURNAL   Biochem J. 2021 Jul 16;478(13):2425-2443. doi: 10.1042/BCJ20210200.
  PUBMED    34198323
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2021-07-16- 16"; volume: "478"; issue: "13"; 
            pages: "2425-2443"
COMMENT     Sequence Label: pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8
            (S...
FEATURES             Location/Qualifiers
     source          1..11595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    98..146
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    505..639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(772..837)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(844..861)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(3719..3810)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3905..3953
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    4051..4084
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(4480..4485,4486..4502,4503..4508)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4697..5230
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  5297..5521
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(5655..5672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5826..6414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5906..5925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6585..7376,7377..7445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7208..7227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7446..7550)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7576..8030)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7713..7734)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7924..7943
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(8330..8989)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8904..8923
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8990..9092)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     mobile_element  9477..9642
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    9920..9968
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    10327..10461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(join(11507..11595,1..3))
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
ORIGIN
        1 gatctattaa tattccggag taggtcgatt taaatcttta gaccatagag cgttcacttg
       61 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacacccgtg
      121 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
      181 ccgtgtttca gttagcctcg ataaaaaccc cgattgagat atagttttta attaacccgt
      241 agtggctatg gcagggcttg ccgccccgac gttggctgcg agccctgggc cttcacccga
      301 acttgggggt tggggtgggg aaaaggaaga aacgcgggcg tattggtccc aatggggtct
      361 cggtggggta tcgacgtaac cccgattgag atatagattt atttaaatgg ttatgatagt
      421 tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga
      481 tcttctaggc tcaagcagtg atcagatcca gacatgataa gatacattga tgagtttgga
      541 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      601 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
      661 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
      721 aaatgtggta tggctgatta tgatcctcta gtacttctcg acaagctttt acttgtcatc
      781 gtcgtctttg tagtcgatgt catgatcctt gtaatcgccg tcgtggtcct tgtagtcaga
      841 accgtgatgg tggtgatggt gggagccacc ctgcagcacg gtgtgagggg tgtacatagc
      901 ctcgtagaat tcgggctccc agtaacgaga ggtgttgtcg ttggtcagca tcacggagta
      961 catgtccagc atgtgtccgg tcagctcgtc gtgcagcttg cggatgtact gcaggtacag
     1021 gtggaacacg tcagcgtact cttgattggg gtgcttagtc agagggtaag cgtcgatagc
     1081 cagggacacg aaacgctcga tcatcagggt tccgtcggtc ttgacgatgt catccacgaa
     1141 gcaaccagcg ccgaggatac gagaaggatc aggataaggc aggtacacgt aatcgtcgcc
     1201 ctgcttgacc agcatggtgt gctgggagca gaactcgtgg ggacccttag tcaggtcagt
     1261 ctcggtccag cacttagcct cggacatgaa cacgttgttc tggtagtaca gcacggactt
     1321 gaagttcttg atggaagcca ccaggccttg ggaagcgtag gtggagttga agcacacgac
     1381 agcgtcgtcg gacaggatca tcatgctgaa gtgcttgcgc aggtaagcgt agaactcgtt
     1441 cacgaagtca gtgtcgacgt cacggttacg gtacaggcac tcgtacagac ggtgctgcag
     1501 gttacgcacg tatttgtcag cgatcttgtt gccgtcggtg gacagcagag cgttcacgtt
     1561 agcggtcaca gcctggcaga tgttgaacac ggagttagcg taagcggtgg tagcgtcgcc
     1621 agaggaagta ccaccaggct tcacgtacag ggaaccaccg cacatgacca tctcggacag
     1681 cacctgagcg cactcgttag ccagacggta gaaacggtga gacagggagc agcaagtagt
     1741 gtgcttgcga gccagcacca gggaagccat gatacgcagc atgttaggca tagcacgatc
     1801 gcacttgggg tagtcccagc ccatcaggtg agggttttcc acgtcggagt acacggtttt
     1861 cagcatgttg tgccagccac cgtagaactt ggaagtaccg atgaccacgg tagcaccgcg
     1921 ggtagcagcg attgacttca gcagcttctg gtggaactga cggttggtca tggtggagca
     1981 gatggagaca ccagccacgg tacgagcacg gttcttagcg gagatggcgt atttcaggtt
     2041 catctgagtg atggtgggga tcacgttacg cttggtgtaa gcgaacagag cgtcctggtc
     2101 ctcgtaggac atggagtcgt agtacagacg agctttgccc catttgttga aggggaagcc
     2161 agcggacttg tccaggttgt tcacgatcac ttgattggcg ttgatgcaac caccgtcgta
     2221 gcagtcgaag tacttgtcca ccacctcgac cacgaacagc agctgacgga tgtcgcacat
     2281 ggtgggcaag ttgtaacggt agtagtcgta gtcggagata gcagcgttgc cgtcttgagc
     2341 gaagaagaag tgcttcagct cgacggagga gccttctttg aagaagccct tggacacggc
     2401 gaaatcgtag aagtccttgt tgaaattgcc gggcttcacg gtctggaagg ccacgttgtt
     2461 agtcagggca gccacggaga agcaagtggt acgcttgtcc agcagcaggt ttccggaagc
     2521 agcatgcata gcggggtcag cagcgtagac cagcagctct ttgaaggaca gacgagagga
     2581 gtggaggttg acgtcctggt tatgcacgac acccagttca cggaagtggt aaccggtgga
     2641 caccacgaaa gggacaccgt ccacgaagat cttgcgcacg agaggaccga aggaggttgg
     2701 agggaacaca gtggagaaca gcacgttgaa gttagcgcag tgcaggatgc aacggtcgtc
     2761 caggcagttc acgcagttag gatggtaggt ctggtcccag tacttgaagt aacggtcgaa
     2821 cagcttcagg cgttcctcgg tgaaatcgta cttcagcaag tcccacttga tgtaaggctt
     2881 ggtcaggtcg gtgtccacgt gagactcagc ggtcagagca cgggtcagag tcaggatggg
     2941 catcagcagg gagtagtaag agtccaccac ggggacacca gaaccagggg tagtctggat
     3001 gaagtcgccg aaatcatacc aattgccgtt caggtcctgg ttgtccaggg tcagcactcc
     3061 cacgatacca gcgttacgca tagcgtcgca gaactgcaca gtcttgagca gagcctgacg
     3121 cacacgctct cccaggttag cgtacacacg caggatgtcg gggttctcga cgaagtcgta
     3181 ccaatccttc ttgttgaagt agtcgtcgtc acagcagttg taggtgacca ggatctcttt
     3241 cagggtgtcg cagtttccct catcgaagtg gcgcagagcg tacaccaggt cagccatggt
     3301 gtacttggtc agacgctgac gagagatgtg gggcaccatg tcgccgtcga tacggaactt
     3361 gaagaagtcg tgcttagcca cagcggggca gtccttcagc aggttgtaga tagtttcctc
     3421 gtgctggtag ttggagaagg tgtgacgctt gaccacgaag taggagtcga tcaggttgtc
     3481 gtcctcgtcc ttctcttgga agcggcagca gttggtcttg aggaacttag cgaaaccggc
     3541 caccttgtcg ttgtagatgt cgaaagcacg gtacaccacg tcggtagagg ttccagtacc
     3601 gcaaggagtc agacgagcag cagagacacc gcacacacgg ttcaggaagg actgagcgtc
     3661 agcagacatg gtggctggat ccgcgcccga tggtgggacg gtatgaataa tccggaatat
     3721 ttataggttt ttttattaca aaactgttac gaaaacagta aaatacttat ttatttgcga
     3781 gatggttatc attttaatta tctccatgat ctattaatat tccggagtag gtcgatttaa
     3841 atctttagac catagagcgt tcacttgaca gagtgccagc cctgggaccg aaccccgcgt
     3901 ttatgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg ccgtcatagc
     3961 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctcgata aaaactttag
     4021 accatagagc gtttaattaa cctaggccag ataacttcgt ataatgtatg ctatacgaag
     4081 ttatggtacc gcggccgcgt agaggatctg ttgatcagca gttcaacctg ttgatagtac
     4141 aaacaaagct gtagccaacc actagaacta tagctagagt cctgggcgaa caaacgatgc
     4201 tcgccttcca gaaaaccgag gatgcgaacc acttcatccg gggtcagcac caccggcaag
     4261 cgccgcgacg gccgaggtct accgatctcc tgaagcaagg gcagatccgt gcacagcacc
     4321 ttgccgtaga agaacagcaa ggccgccaat gcctgacgat gcgtggagac cgaaaccttg
     4381 cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg
     4441 tgacgcacac cgtggaaacg gatgaaggca caaacccagt tgacataagc ctgttcggtt
     4501 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     4561 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
     4621 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     4681 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
     4741 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
     4801 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
     4861 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
     4921 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
     4981 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
     5041 cgagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
     5101 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
     5161 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     5221 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
     5281 attgtcacaa cgccgcgaat atagtcttta ccatgccctt ggccacgccc ctctttaata
     5341 cgacgggcaa tttgcacttc agaaaatgaa gagtttgctt tagccataac aaaagtccag
     5401 tatgcttttt cacagcataa ctggactgat ttcagtttac aactattctg tctagtttaa
     5461 gactttattg tcatagttta gatctatttt gttcagttta agactttatt gtccgcccac
     5521 acccgcttac gcagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc
     5581 ggctggtcta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5641 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6241 tggcctaact acggctacac tagaagaata gtatttggta tctgcgctct gctgaagcca
     6301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7561 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     7621 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     7681 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     7741 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     7801 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     7861 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     7921 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     7981 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     8041 atcaggctct gggaggcaga ataaatgatc atatcgtcaa ttattacctc cacggggaga
     8101 gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     8161 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg
     8221 acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc
     8281 aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
     8341 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     8401 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     8461 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     8521 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     8581 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     8641 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     8701 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     8761 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     8821 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     8881 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     8941 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     9001 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     9061 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
     9121 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     9181 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt
     9241 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
     9301 tcagttcagg ctgcaaataa gcgttgatat tcagtcaatt acaaacatta ataacgaaga
     9361 gatgacagaa aaattttcat tctgtgacag agaaaaagta gccgaagatg acggtttgtc
     9421 acatggagtt ggcaggatgt ttgattaaaa acataacagg aagaaaaatg ccccgctgtg
     9481 ggcggacaaa atagttggga actgggaggg gtggaaatgg agtttttaag gattatttag
     9541 ggaagagtga caaaatagat gggaactggg tgtagcgtcg taagctaata cgaaaattaa
     9601 aaatgacaaa atagtttgga actagatttc acttatctgg ttcggatctc ctgattgaaa
     9661 catcaggcat cattaggttt ttaattaacc cgtagtggct atggcagggc ttgccgcccc
     9721 gacgttggct gcgagccctg ggccttcacc cgaacttggg ggttggggtg gggaaaagga
     9781 agaaacgcgg gcgtattggt cccaatgggg tctcggtggg gtatcgacgt aaccccgatt
     9841 gagatataga tttaaatctt tagaccatag agcgttcact tgacagagtg ccagccctgg
     9901 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     9961 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
    10021 cgataaaaac gtgcaatagt atccagtttt taattaaccc gtagtggcta tggcagggct
    10081 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg gttggggtgg
    10141 ggaaaaggaa gaaacgcggg cgtattggtc ccaatggggt ctcggtgggg tatcgacgta
    10201 accccgattg agatatagat ttatttaaat ggttatgata gttattgctc agcggtggca
    10261 gcagccaact cagcttcctt tcgggctttg ttagcagccg gatcttctag gctcaagcag
    10321 tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
    10381 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
    10441 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
    10501 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
    10561 tatgatcctc tagtacttct cgacaagctt ttactgcagc ttcacagcgg agttagcacg
    10621 cagagcagtc acgatcaggg gccaagccag gttgggagag ttgtccatgg agatctcgga
    10681 cagctgcacg atcttggagt cagcgtccac cacttgctgg atctcccaca gagcggaagc
    10741 gtaggtgaag gtggtgccgt cgcaagtgtt cttgtaggtg ttgtagtcgg ggatgaccac
    10801 catcagctta gcagcggtgg tcagagggat gatgttcagg ggcacgcaac cgtcacgagc
    10861 gttgttgatg atgttgttca gagcgtcgtt gtccagcttg cggagcatgg tgaacagcat
    10921 ggtctgcata gcggaggtca ctttagcacg cttgtcctcg gaacgagcct gcttgtacat
    10981 ctgggtcata gcctggtcag ccatcttctc gagcttgcgc tgcatagcag cgtcacggtc
    11041 gaactcggac ttggccacgt tcagggactt tttcagcttc ttcagcacca cctcagagtc
    11101 gccgttagcc acagcctgct cgtaagcctc ttgagcggta gcgaaagcag cgtaggaggg
    11161 cagagaggag aactcggaag cgatagcgga gccaccagat ccaccctgca gggtagcacg
    11221 gttgtccagc atctcttcgc acagcttgtt gatgtccaca gcaccttgca tagacagcag
    11281 gacggacagc agggacacca tcttctcgaa agcctcggtg gtgtccttag ccagcaggat
    11341 gtcgttgtgc agttggacgc actgagccca cagcttgctg gaggattcca cacgcagctg
    11401 ttgcagcaca gacagcagca ccacggaggt gcacttcacg tcggacatct tggacatggt
    11461 ggctggatcc gcgcccgatg gtgggacggt atgaataatc cggaatattt ataggttttt
    11521 ttattacaaa actgttacga aaacagtaaa atacttattt atttgcgaga tggttatcat
    11581 tttaattatc tccat
//
