LOCUS       sequence-33399-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 S208A
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    143..277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(180..199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     234..253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctggg taccgggccc aatgcattgg
       61 cgccgcggcg cgccgcgaat taaaaaacct cccacacctc cccctgaacc tgaaacataa
      121 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      241 gtccaaactc atcaatgtat cttatcatgt ctggatctac gtaatacgac tcactatagt
      301 tctagaggct cgagaggcct tgcggccgcg gccagattgg cctgtcgacg aattcctgca
      361 gcccgatgtc tctaagacac actggaacag cggatgcttg gggagcccat ctgggtgagg
      421 accttgtcaa gccactgcaa aggcccattc aggtgcagct caatccagca gggggtactg
      481 gtcacagtct gtctcctgta ctccgctccc cagcctttga cgaagctcat gcggatggtg
      541 cacattcggg tcaactggta gacagcctca aagccctggt tgaccgactg ggccaggagg
      601 gcagcgaact cctggttgtt gaagatcttc aggttgcatc ctggtgggat cttgcagacg
      661 gtggccgggt gccagccata gcgctggtta cagttgggag actggac
//
