LOCUS       sequence-333991-        9217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of DmBelle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pETM-60-NusA-DmBelle_B
FEATURES             Location/Qualifiers
     source          1..9217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             88..1569
                     /codon_start=1
                     /gene="nusA"
                     /product="transcription elongation protein (N utilization 
                     substance protein A)"
                     /label=NusA
                     /note="highly soluble in E. coli"
                     /translation="MKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVR
                     VQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRI
                     TTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILR
                     EDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEI
                     KAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQ
                     FVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTV
                     DDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDE
                     PTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDL
                     AEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     CDS             1585..1602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1612..1632
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4082..4099
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4152..4170)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4166..4213
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4250..4705
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4337..4356)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4547..4568
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4797..5612)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4797..5612)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5520..5539
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5604..5623
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5734..6322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6223..6242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6476..6493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6508..6647
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6573..6592)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6733..6755
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6749..6940)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7749..8831)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8793..8812
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8832..8909)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9115..9134
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg aaagaaattt tggctgtagt tgaagccgta
      121 tccaatgaaa aggcgctacc tcgcgagaag attttcgaag cattggaaag cgcgctggcg
      181 acagcaacaa agaaaaaata tgaacaagag atcgacgtcc gcgtacagat cgatcgcaaa
      241 agcggtgatt ttgacacttt ccgtcgctgg ttagttgttg atgaagtcac ccagccgacc
      301 aaggaaatca cccttgaagc cgcacgttat gaagatgaaa gcctgaacct gggcgattac
      361 gttgaagatc agattgagtc tgttaccttt gaccgtatca ctacccagac ggcaaaacag
      421 gttatcgtgc agaaagtgcg tgaagccgaa cgtgcgatgg tggttgatca gttccgtgaa
      481 cacgaaggtg aaatcatcac cggcgtggtg aaaaaagtaa accgcgacaa catctctctg
      541 gatctgggca acaacgctga agccgtgatc ctgcgcgaag atatgctgcc gcgtgaaaac
      601 ttccgccctg gcgaccgcgt tcgtggcgtg ctctattccg ttcgcccgga agcgcgtggc
      661 gcgcaactgt tcgtcactcg ttccaagccg gaaatgctga tcgaactgtt ccgtattgaa
      721 gtgccagaaa tcggcgaaga agtgattgaa attaaagcag cggctcgcga tccgggttct
      781 cgtgcgaaaa tcgcggtgaa aaccaacgat aaacgtatcg atccggtagg tgcttgcgta
      841 ggtatgcgtg gcgcgcgtgt tcaggcggtg tctactgaac tgggtggcga gcgtatcgat
      901 atcgtcctgt gggatgataa cccggcgcag ttcgtgatta acgcaatggc accggcagac
      961 gttgcttcta tcgtggtgga tgaagataaa cacactatgg acatcgccgt tgaagccggt
     1021 aatctggcgc aggcgattgg ccgtaacggt cagaacgtgc gtctggcttc gcaactgagc
     1081 ggttgggaac tcaacgtgat gaccgttgac gacctgcaag ctaagcatca ggcggaagcg
     1141 cacgcagcga tcgacacctt caccaaatat ctcgacatcg acgaagactt cgcgactgtt
     1201 ctggtagaag aaggcttctc gacgctggaa gaattggcct atgtgccgat gaaagagctg
     1261 ttggaaatcg aaggccttga tgagccgacc gttgaagcac tgcgcgagcg tgctaaaaat
     1321 gcactggcca ccattgcaca ggcccaggaa gaaagcctcg gtgataacaa accggctgac
     1381 gatctgctga accttgaagg ggtagatcgt gatttggcat tcaaactggc cgcccgtggc
     1441 gtttgtacgc tggaagatct cgccgaacag ggcattgatg atctggctga tatcgaaggg
     1501 ttgaccgacg aaaaagccgg agcactgatt atggctgccc gtaatatttg ctggttcggt
     1561 gacgaagcga ctagtggttc tggtcatcac catcaccatc actccgcggg tgagaatctt
     1621 tattttcagg gcgccatggc aatgagtaat gctattaacc aaaatggcac aggtctagag
     1681 cagcaagttg ctggtctgga cttgaatggc ggctctgctg actacagcgg ccccataacc
     1741 agcaagactt ccactaactc ggtcaccggt ggtgtgtatg tgcccccgca ccttcgtggt
     1801 ggtggtggca ataacaacgc agcggacgca gagagccagg gccaaggaca gggccagggt
     1861 cagggcttcg actccagatc cgggaatccg cgccaggaga ctagggaccc tcaacagtcg
     1921 cgcggaggcg gaggcgaata ccgcagaggc ggcggcggtg gtggtcgcgg cttcaatcgc
     1981 cagagcggcg actacggtta cggaagcggc ggcggaggac gacgtggagg cggtggacgt
     2041 ttcgaggaca actacaacgg tggtgaattc gattcgcgtc gcggcggtga ctggaatcgc
     2101 agcggcggag gaggtcgagg atttggacgc ggaccatcgt accgcggcgg tggcggcgga
     2161 agcggtagca acctcaacga gcagactgcc gaggatggcc aggcacagca gcagcagcag
     2221 ccgcgcaacg atcgttggca ggagccagaa cgtcctgccg gattcgatgg cagcgagggc
     2281 ggacagtctg ccggcggcaa cagaagctac aacaatcgcg gcgagcgcgg aggcggcggt
     2341 tacaacagcc gctggaagga gggcggcggc tcaaacgtcg actacacaaa gctgggcgcc
     2401 cgagatgagc gcctggaggt ggagctgttc ggcgtgggca atacgggaat caattttgac
     2461 aaatacgagg atatacccgt ggaggcgacg ggccagaatg tgcccccgaa catcacatcg
     2521 ttcgatgatg tgcagctgac ggagattatc cgcaacaacg tggccttagc acgttatgac
     2581 aaaccgacac cggtgcagaa gcacgccatt ccgatcatta tcaatggccg ggatctaatg
     2641 gcctgcgccc agactggatc cggcaagacg gccgccttcc tggtgccgat tctcaaccag
     2701 atgtacgagc tgggacatgt gcccccgccg caaagcaccc ggcagtacag ccggcgcaag
     2761 cagtatccgt tgggtctggt gctggcgccg acccgcgaac tggccaccca gatctttgag
     2821 gaggccaaga agttcgccta tcgctcccgg atgcgcccgg cagtgctgta cggcggaaac
     2881 aatactagcg agcagatgcg cgagctggac cgcggttgcc atctgattgt ggccacaccc
     2941 ggtcgcctag aggacatgat cacacgcgga aaggtgggac tggagaacat tcgattcctt
     3001 gtactggatg aggctgatcg tatgttggac atgggtttcg agccgcagat ccgtcgcatc
     3061 gtcgaacagc taaacatgcc gccaaccgga cagcgccaga cgcttatgtt ctcggccaca
     3121 ttccccaagc agatccagga gttagccagc gatttcctca gcaactacat tttcttggcc
     3181 gttggtcgtg tgggctccac atctgagaac attacgcagg caatcctgtg ggtctatgaa
     3241 cccgacaagc gctcgtatct gctggatctg ctctcgtcta tccgcgatgg ccccgaatac
     3301 accaaagaca gcctaacact gatctttgtg gagactaaga agggtgcaga ctcgctggag
     3361 gagttcctgt accagtgcaa tcatcccgtc actagtatcc acggtgatcg cacgcagaag
     3421 gagcgcgagg aggcactgcg ctgcttccga tcgggcgact gccccatcct ggtggccaca
     3481 gccgtggccg ctcgtggcct ggacattccg cacgttaagc acgttattaa ctttgacctg
     3541 ccctcggatg tggaggagta tgtccaccgt atcgggcgta ccggacgtat gggtaatctt
     3601 ggtgtggcca cctccttttt caacgaaaag aaccgcaaca tttgctccga tctcttggag
     3661 ctgctgatcg aaacgaagca ggaggtcccc agtttcatgg aggatatgag ttcggatcgc
     3721 ggacatggcg gagccaagcg ggctggacga ggcggtggtg gacgttatgg tggcggcttc
     3781 ggctccaggg attaccgtca aagctctggc ggcggcggtg gcggacgcag tggaccgcca
     3841 ccacgtagcg gtggttccgg atcaggaggc ggcggcggca gttaccgcag caatggaaac
     3901 tcgtacggcg gcaattcggg cggcggtgga tactatggcg gcggcgccgg tggtggctcc
     3961 tatggcggct cctacggcgg cggcagtgcc tcgcactctt cgaacgcacc cgactggtgg
     4021 gctcaatgag gtaccggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga
     4081 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     4141 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     4201 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
     4261 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4321 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4381 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4441 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4501 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4561 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4621 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4681 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4741 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag
     4801 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     4861 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     4921 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     4981 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     5041 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     5101 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     5161 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     5221 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     5281 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     5341 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     5401 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     5461 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     5521 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     5581 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     5641 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5881 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6121 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6181 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6241 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6301 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6361 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6421 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6481 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     6541 cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     6601 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     6661 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6721 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6781 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
     6841 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     6901 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     6961 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     7021 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     7081 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     7141 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     7201 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     7261 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     7321 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     7381 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct
     7441 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
     7501 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
     7561 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
     7621 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     7681 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
     7741 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
     7861 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
     7921 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
     7981 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     8041 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     8101 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     8161 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     8221 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     8281 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     8341 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     8401 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     8461 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     8521 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     8581 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     8641 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     8701 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     8761 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     8821 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     8881 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
     8941 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
     9001 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     9061 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     9121 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     9181 tccggcgtag aggatcgaga tctcgatccc gcgaaat
//
