LOCUS       sequence-334-exp        5553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VSV-G expressing envelope vector; recommended for the FELIX 
            (FIV-based) vector system from the Nolan lab.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5553)
  TITLE     Nolan Lab Plasmids
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCI-VSVG
FEATURES             Location/Qualifiers
     source          1..5553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1076..1123,1124..2611)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTAIQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITQSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    2665..2786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2676..2695)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2730..2749
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2967..3422
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3054..3073)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3264..3285
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3491..3510)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3610..3632
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3670..3688)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3756..3860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3861..3929,3930..4721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4079..4098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4892..5480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5381..5400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattctg acactatgaa
     1081 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt
     1141 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc
     1201 aagctcagat ttaaattggc ataatgactt aataggcaca gccatacaag tcaaaatgcc
     1261 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac
     1321 tacttgtgat ttccgctggt atggaccgaa gtatataaca cagtccatcc gatccttcac
     1381 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa
     1441 tccaggcttc cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat
     1501 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc
     1561 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac
     1621 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat
     1681 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag
     1741 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca
     1801 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc
     1861 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc
     1921 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga
     1981 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc
     2041 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt
     2101 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat
     2161 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt
     2221 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat
     2281 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga
     2341 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg
     2401 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa
     2461 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct
     2521 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac
     2581 agacatagag atgaaccgac ttggaaagta actcaaatcc tgcactctag agtcgacccg
     2641 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     2701 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2761 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2821 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     2881 tcgataagga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2941 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3001 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3061 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3121 ggggctccct ttagggttcc gatttagagc tttacggcac ctcgaccgca aaaaacttga
     3181 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3241 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3301 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3361 aaatgagctg atttaacaaa tatttaacgc gaattttaac aaaatattaa cgtttacaat
     3421 ttcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     3481 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3541 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3601 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3661 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3721 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5521 gctggccttt tgctcacatg gctcgacaga tct
//
