LOCUS       sequence-33400-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 S213A
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga ttcgaattca
      121 ccatggacta caaggacgac gatgacaagt cgtccatcct gcctttcact cccccgatcg
      181 tgaagcgcct gctgggctgg aagaagggcg agcagaacgg gcaggaggag aaatggtgcg
      241 agaaggcggt caagagcctg gtcaagaaac tcaagaagac ggggcagctg gacgagctgg
      301 agaaggccat caccacgcag aacgtcaaca ccaagtgcat caccatcccc aggtccctgg
      361 atggccggtt gcaggtgtcc catcggaagg ggctccctca tgtcatctac tgccgcctgt
      421 ggcgatggcc agacctgcac agccaccacg agctgcgggc catggagctg tgtgagttcg
      481 ccttcaatat gaagaaggac gaggtctgcg tgaatcccta ccactaccag agagtagaga
      541 caccagttct acctcctgtg ttggtgccac gccacacaga gatcccggcc gagttccccc
      601 cactggacga ctacagccat tccatccccg aaaacactaa cttccccgca ggcatcgagc
      661 cccagagcaa tattccagag accccacccc ctggctacct gagtgaagat ggagaaacca
      721 gtgaccacca gatgaaccac agcatggacg caggttctcc aaacctatcc ccgaatccga
      781 tggccccagc acataataac ttggacctgc agccagttac ctactgcgag ccggccttct
      841 ggtgctccat ctcctactac gagctgaacc agcgcgtcgg ggagacattc cacgcctcgc
      901 agccatccat gactgtggat ggcttcaccg acccctccaa ttcggagcgc tt
//
