LOCUS       sequence-33401-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 S213A
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     43..63
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        45..63
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    180..314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(217..236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     271..290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
       61 agggaacaaa agctgggtac cgggcccaat gcattggcgc cgcggcgcgc cgcgaattaa
      121 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      301 atcatgtctg gatctacgta atacgactca ctatagttct agaggctcga gaggccttgc
      361 ggccgcggcc agattggcct gtcgacgaat tcctgcagcc cgatgtctct aagacacact
      421 ggaacagcgg atgcttgggg agcccatctg ggtgaggacc ttgtcaagcc actgcaaagg
      481 cccattcagg tgcagctcaa tccagcaggg ggtactggtc acagtctgtc tcctgtactc
      541 cgctccccag cctttgacga agctcatgcg gatggtgcac attcgggtca actggtagac
      601 agcctcaaag ccctggttga ccgactgggc caggagggca gcgaactcct ggttgttgaa
      661 gatcttcagg ttgcatcctg gtgggatctt gcagacggtg gccgggtgcc agccatagcg
      721 ctggttacag ttgggagact ggacaaaaat agcgctgtca ctgaggcact ctgcgaagac
      781 ctcccctccg atgtagtaga gccgcacgcc tcttccgatg tgtctccgtg tcagctccac
      841 tgctgcattc ctgttgacat tggagagcag cccctaggca gaagcgctcc gaattggagg
      901 ggtcggtgaa gccatcccac agtcatggat ggcttgcgaa g
//
