LOCUS       sequence-33405-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM
  TITLE     Localization of ASH1 mRNA particles in living yeast.
  JOURNAL   Mol Cell. 1998 Oct . 2(4):437-45.
  PUBMED    9809065
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Oct . 2(4):437-45."
COMMENT     Sequence Label: pG14-MS2-GFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(392..403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(404..430)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(404..430)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(434..454)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(468..732)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(552..575)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aatcttccct ttgagcggcc gcgtagatgc cggagtttgc tgcgattgct gagggaatcg
       61 ggtttccatc ttttaggaga ccttgcattg ccttaacaat aagctcgcag tcggaattcg
      121 tggcgaaaat tggaatggtt agttccatat ttaagtacga acgccaggcg cctttaggca
      181 cctcgacttt gatggtgtat ttgcgattct gcgcagagct ctgacgaacg ctacaggtta
      241 ctttgtaagc ctgtgaacgc gagttagagc tgatccattc agcgatcccg ttagcgaagt
      301 tgcttggggc gacagtcacg tcgccagttc cgccattgtc gacgagaacg aactgagtaa
      361 agttagaagc cattttaacg gctagcatat ggcggccttc gatggcgtag tcgggcacgt
      421 cgtaggggta gccaactttt cttttctttt ttggcatggt ggatccgtcg aaactaagtt
      481 cttggtgttt taaaactaaa aaaaagacta actataaaag tagaatttaa gaagtttaag
      541 aaatagattt acagaattac aatcaatacc taccgtcttt atatacttat tagtcaagta
      601 ggggaataat ttcagggaac tggtttcaac cttttttttc agctttttcc aaatcagaga
      661 gagcagaagg taatagaagg tgtaagaaaa tgagatagat acatgcgtgg gtcaattgcc
      721 ttgtgtcatc atttactcca ggcaggttgc atcactccat tgaggttgtg cccgtttttt
      781 gcctgtttgt gcccctgttc tctgtagttg cgctaagaga atggacctat gaactgatgg
//
