LOCUS       sequence-334113-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Chen M, Ma Z, Wu X, Mao S, Yang Y, Tan J, Krueger CJ, Chen AK
  TITLE     A molecular beacon-based approach for live-cell imaging of RNA 
            transcripts with minimal target engineering at the single-molecule 
            level.
  JOURNAL   Sci Rep. 2017 May 8;7(1):1550. doi: 10.1038/s41598-017-01740-1.
  PUBMED    28484218
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-05-8"; volume: "7(1):1550. doi"; pages: " 
            10.1038/s41598-017-01740-1"
COMMENT     Sequence Label: pEGFP-N1-4x
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      220..229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(226..228,229..231,232..945)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(271..292)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(532..551)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     879..900
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caggagttgt gtttgtggac gaagagcacc agccagctga tcgacctcga caggagttgt
       61 gtttgtggac gaagagcacc agccagctga tcgacctcga caggagttgt gtttgtggac
      121 gaagagcacc agccagctga tcgacctcga caggagttgt gtttgtggac gaagagcacc
      181 agccagctga tcgacctcga gccgagggat ccaccggtcg ccaccatggt gagcaagggc
      241 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      301 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      361 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      421 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      481 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      541 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      601 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      661 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      721 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      781 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      841 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      901 accgccgccg ggatcactct cggcatggac gagctgtaca agtaa
//
