LOCUS       sequence-334114-        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing EGFP mRNA fused with 8 tandem repeats of a 50-base 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Chen M, Ma Z, Wu X, Mao S, Yang Y, Tan J, Krueger CJ, Chen AK
  TITLE     A molecular beacon-based approach for live-cell imaging of RNA 
            transcripts with minimal target engineering at the single-molecule 
            level.
  JOURNAL   Sci Rep. 2017 May 8;7(1):1550. doi: 10.1038/s41598-017-01740-1.
  PUBMED    28484218
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-05-8"; volume: "7(1):1550. doi"; pages: " 
            10.1038/s41598-017-01740-1"
COMMENT     Sequence Label: pEGFP-N1-8x
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      415..424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(421..423,424..426,427..1140)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(466..487)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(727..746)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1074..1095
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caggagttgt gtttgtggac gaagagcacc agccagctga tcgacctcga caggagttgt
       61 gtttgtggac gaagagcacc agccagctga tcgacctcga caggagttgt gtttgtggac
      121 gaagagcacc agccagctga tcgacctcga caggagttgt gtttgtggac gaagagcacc
      181 agccagctga tcgacctcga caggagttgt gtttgtggac gaagagcacc agccagctga
      241 tcgacctcga caggagttgt gtttgtggac gaagagcacc agccagctga tcgacctcga
      301 caggagttgt gtttgtggac gaagagcacc agccagctga tcgacctcga caggagttgt
      361 gtttgtggac gaagagcacc agccagctga tcgacctcga gggatccacc ggtcgccacc
      421 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      481 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      541 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      601 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      661 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      721 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      781 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      841 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      901 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      961 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1021 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1081 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
//
