LOCUS       sequence-334129-        2788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of mTagBFP2 blue fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2788)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7A)-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..2788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             101..811
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     rep_origin      complement(1033..1621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1113..1132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1792..2583,2584..2652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2415..2434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2653..2757)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatacgact cactataagg acatttgctt ctgacacaac tgtgttcact agcaacctca
       61 aacagaattc gagctcggta cccggggatc ctctagagtc atggtgtcta agggcgaaga
      121 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
      181 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
      241 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
      301 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
      361 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
      421 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
      481 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
      541 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
      601 gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc
      661 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
      721 ggaggccaac aacgaaacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
      781 cctccctagc aaactggggc acaagcttaa ttaggacctg caggcatgca agcttgctcg
      841 ctttcttgct gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac
      901 tgggggatat tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt
      961 tcattgcaaa accatgggag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1441 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1501 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1801 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1861 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1921 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1981 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2041 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2101 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2161 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2221 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2281 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2341 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2401 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2641 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2701 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2761 atttccccga aaagtgccac ctgacgtc
//
