LOCUS       sequence-33413-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Kienker PK, Jakes KS, Finkelstein A
  TITLE     Protein translocation across planar bilayers by the colicin Ia 
            channel-forming domain: where will it end?
  JOURNAL   J Gen Physiol. 2000 Oct . 116(4):587-98.
  PUBMED    11004207
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol. 2000 Oct . 116(4):587-98."
COMMENT     Sequence Label: pKSJ155
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagccata tgctcgagga gaaaagaaaa caggatgaac
      121 tgaaggcaac gaaagacgca attaatttca caacagagtt cctgaaatca gtttcagaaa
      181 aatatggtgc aaaagctgag cagttagcca gagagatggc cgggcaggct aaagggaaga
      241 aaatacgtaa tgttgaagag gcattaaaaa cgtatgaaaa gtaccgggct gacattaaca
      301 aaaaaattaa tgcaaaagat cgtgcagcga ttgccgcagc ccttgagtct gtgaagctgt
      361 ctgatatatc gtctaatctg aacagattca gtcggggact gggatatgca ggaaaattta
      421 caagtcttgc tgactggatc actgagtttg gtaaggctgt ccggacagag aactggcgtc
      481 ctctttttgt taaaacagaa accatcatag caggcaatgc cgcaacggct cttgtggcac
      541 tggtcttcag tattcttacc ggaagcgctt taggcattat cgggtatggt ttactgatgg
      601 ctgtcaccgg tgcgctgatt gatgaatcgc ttgtggaaaa agcgaataag ttctggggta
      661 tttaaataaa atataagaca ggctgtctat cttacagaca gcctttttat aataacagaa
      721 agaatatata tcatacttaa cggaagagat aatatgaaaa cagtacccgt ataaaccgca
      781 agcaacccca tttttccagg tgtatcagca aaaaaaaccg ctgttccaga aatcaggcc
//
