LOCUS       sequence-334143-       10756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Equalizer-L plasmid that encodes mScarlet-I to report the circuit 
            output levels. This plasmid also encodes a separate mCitrine 
            expression cassette to monitor plasmid dosage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Yang J, Lee J, Land MA, Lai S, Igoshin OA, St-Pierre F
  TITLE     A synthetic circuit for buffering gene dosage variation between 
            individual mammalian cells.
  JOURNAL   Nat Commun. 2021 Jul 5;12(1):4132. doi: 10.1038/s41467-021-23889-0.
  PUBMED    34226556
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-5"; volume: "12(1):4132. doi"; pages: " 
            10.1038/s41467-021-23889-0"
COMMENT     Sequence Label: Equalizer-L (mScarlet-I) plasmid with onboard
            ...
FEATURES             Location/Qualifiers
     source          1..10756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    814..832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          1041..1613
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1647..1656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2331..2387
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2394..3089
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2527..2545)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2795..2814
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           3298..3312
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3340..3468
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(3558..3578)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    3937..4161
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4294..4313)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4445..4492
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4500..5522
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4500..5522
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5652..5773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5822..5838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5822..5838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5835..5857)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5846..5862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5870..5876,5877..5894,5895..5900))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5915..5936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6051..7232
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6281..7223
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7180..7200
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(7254..7256,7257..7259,7260..7973)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7299..7320)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7560..7579)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7907..7928
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(7992..8010)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7993..8010)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    8171..8292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    8715..8939
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(9001..9589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9081..9100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9760..10551,10552..10620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10383..10402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10621..10725)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tactctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcatccctat cagtgataga gatcagatct
      841 ccctatcagt gatagagagc tgttttgacc tccatagaag acaccgggac cgatccagcc
      901 tccggactct agcgtttaaa cttaagctgg gtacccgggg atcctctagg gcctctgagc
      961 tattccagaa gtagtgaaga ggcttttttg gaggcctagg cttttgcaaa aagctccgga
     1021 tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     1081 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     1141 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     1201 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     1261 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     1321 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     1381 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     1441 ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc
     1501 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     1561 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta caggtcctgc
     1621 accgcttgaa gtctttaatt aaaggcgcca ccatggagtc tagactggac aagagcaaag
     1681 tcataaactc tgctctggaa ttactcaatg aagtcggtat cgaaggcctg acgacaagga
     1741 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     1801 gggccctgct cgatgccctg gcaatcgaga tgctggacag gcatcatacc cacttctgcc
     1861 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg
     1921 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     1981 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     2041 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggatc
     2101 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     2161 ttctgagaca agcaattgag ctgttcgacc atcagggagc cgaacctgcc ttccttttcg
     2221 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccaa
     2281 aaaagaagag aaagggtgac ggtgctggtt taattaacat gggaagcgga gctactaact
     2341 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctgct agcatggtga
     2401 gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca
     2461 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2521 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt
     2581 cccctcagtt catgtacggc tccagggcct tcatcaagca ccccgccgac atccccgact
     2641 actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2701 gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg
     2761 tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg
     2821 gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta
     2881 agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca
     2941 aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca
     3001 cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact
     3061 ccaccggcgg catggacgag ctgtacaagt aaccgcttga agtctttaat taaaaagctt
     3121 gtgagtatgt gctcgcttcg gcagcacata tactatgtcg aatgaggctt cagtacttta
     3181 cagaatcgtt gcctgcacat cttggaaaca cttgctggga ttacttcttc aggttaaccc
     3241 aacagaaggc tcgagtgctg ttgacagtga gcgccgcttg aagtctttaa ttaaatagtg
     3301 aagccacaga tgtatttaat taaagacttc aagcggtgcc tactgcctcg gagaattcaa
     3361 ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc tatctctttg
     3421 atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt tcaattgttt
     3481 cctttttttt cctcaggcgg ccgcaatcaa cctctggatt acaaaatttg tgaaagattg
     3541 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3601 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3661 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3721 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3781 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3841 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcctcgagt
     3901 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     3961 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4021 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4081 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4141 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4201 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4261 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4321 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4381 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4441 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     4501 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     4561 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     4621 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     4681 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     4741 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     4801 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     4861 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     4921 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     4981 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     5041 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     5101 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     5161 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     5221 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     5281 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     5341 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     5401 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     5461 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     5521 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5581 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5641 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5701 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5761 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5821 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5881 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5941 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6001 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct gctccggtgc
     6061 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     6121 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     6181 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     6241 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     6301 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacgcc
     6361 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     6421 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     6481 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     6541 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     6601 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     6661 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     6721 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     6781 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     6841 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     6901 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     6961 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     7021 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     7081 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     7141 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     7201 aaagtttttt tcttccattt caggtgtcgt gacataaact gccctagacc accatggtga
     7261 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7321 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7381 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     7441 ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg
     7501 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     7561 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     7621 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     7681 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     7741 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     7801 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     7861 gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     7921 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tagtaatcta
     7981 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagt ccatagtcga
     8041 cggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     8101 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     8161 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8281 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     8341 tttgttaaat cagctcattt tttaaccaat aggccggctg tctgttcagt tatgaagatg
     8401 ccgcatagtt aagccagtat ctgctccctg gtcttctcca tcggatcctg atcaggggag
     8461 ctcacgggga cagccccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc
     8521 tagggggcag cagcgaccgc ccggggctcc gctccggtcc ggcgctccca ccgcatcccg
     8581 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     8641 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg gggatacggg gaaaaccgcg
     8701 gcatatggcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8761 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8821 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8881 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgga
     8941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10741 agtgccacct gacgtc
//
