LOCUS       sequence-334145-         914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused
            with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization
            Signal (NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Vallecillo-Viejo IC, Liscovitch-Brauer N, Montiel-Gonzalez MF, 
            Eisenberg E, Rosenthal JJC
  TITLE     Abundant off-target edits from site-directed RNA editing can be 
            reduced by nuclear localization of the editing enzyme.
  JOURNAL   RNA Biol. 2018 Jan 2;15(1):104-114. doi: 
            10.1080/15476286.2017.1387711. Epub 2017 Nov 13.
  PUBMED    29099293
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15476286.2017.1387711"; journalName: "RNA Biol"; date: 
            "2018-01-2- 2"; volume: "15"; issue: "1"; pages: "104-114"
COMMENT     Sequence Label: NLS-4LNDDE488Q-pCDNA3.1(+)
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             37..57
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             61..81
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             85..105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactaca aggacgacga tgacaaacta gtagctccaa aaaagaagag aaaggtagat
       61 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaggatc cacctccgga
      121 gtaaatgccc gtacgcgccg gcgcgaacgg cgagcagaaa agcaggcaca gtggaaggca
      181 gctaatggcg gagggggaag cgggggcggt ggaagtgggg gtggaggcag caatgcgcgg
      241 acccgccgac gtgaacggcg agcagaaaaa caggcgcaat ggaaagcagc caacgacggc
      301 ggtggaagtg gaggtggtgg aagcggaggc ggtggtagta acgccagaac ccgacgcaga
      361 gagaggaggg cagagaaaca ggcccagtgg aaagccgcta acggaggagg gggatccggt
      421 ggaggcggca gtggaggcgg gggatctaac gcacgaacac gacgacgtga gcgtcgcgct
      481 gagaaacaag ctcaatggaa agctgcaaac ttgcacttgg atcagacgcc atctcgccag
      541 cctattccca gtgagggtct tcagctgcat ttaccgcagg ttttagctga cgctgtctca
      601 cgcctggtcc tgggtaagtt tggtgacctg accgacaact tctcctcccc tcacgctcgc
      661 agaaaagtgc tggctggagt cgtcatgaca acaggcacag atgttaaaga tgccaaggtg
      721 ataagtgttt ctacaggaac aaaatgtatt aatggtgaat acatgagtga tcgtggcctt
      781 gcattaaatg actgccatgc agaaataata tctcggagat ccttgctcag atttctttat
      841 acacaacttg agctttactt aaataacaaa gatgatcaaa aaagatccat ctttcagaaa
      901 tcanagcgag gggg
//
