LOCUS       sequence-334146-       12678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lenti vector encoding the MS2-p300 with a 2A Hygro resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson 
            KE, Ni M, Xu J
  TITLE     Interrogation of enhancer function by enhancer-targeting CRISPR 
            epigenetic editing.
  JOURNAL   Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
  PUBMED    31980609
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-24"; volume: "11(1):485. doi"; pages: " 
            10.1038/s41467-020-14362-5"
COMMENT     Sequence Label: Lenti_MCP-p300_Hygro
FEATURES             Location/Qualifiers
     source          1..12678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2450..3628
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2681..3619
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3576..3596
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3635..3644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3644..4030
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4085..4105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4121..5971
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      5735..5744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5972..5998
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6017..6070
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6074..7093
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    7133..7721
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7186..7206)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7604..7615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7724..7740)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7725..7741)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8246..8426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8452..8469)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8458..8682
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8728..9156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8815..8834)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9025..9046
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9165..9185)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9170..9499
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9350..9485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9412..9431
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9547..9594
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9613..9987
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10117..10238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10154..10173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10208..10227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10287..10303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10287..10303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10300..10322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10311..10327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10335..10341,10342..10359,10360..10365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10380..10401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10518..10535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10689..11277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10769..10788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11448..12239,12240..12308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12071..12090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12309..12413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12488..12507)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagct gcaaagatgg ataaagtttt aaacagagag
     2401 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     2461 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2521 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2581 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2641 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     2701 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     2761 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     2821 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     2881 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     2941 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     3001 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     3061 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     3121 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     3181 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     3241 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatgaagga cgcggcgctc
     3301 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     3361 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     3421 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     3481 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     3541 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     3601 tttttttctt ccatttcagg tgtcgtgacg tacggccacc atggcttcaa actttactca
     3661 gttcgtgctc gtggacaatg gtgggacagg ggatgtgaca gtggctcctt ctaatttcgc
     3721 taatggggtg gcagagtgga tcagctccaa ctcacggagc caggcctaca aggtgacatg
     3781 cagcgtcagg cagtctagtg cccagaagag aaagtatacc atcaaggtgg aggtccccaa
     3841 agtggctacc cagacagtgg gcggagtcga actgcctgtc gccgcttgga ggtcctacct
     3901 gaacatggag ctcactatcc caattttcgc taccaattct gactgtgaac tcatcgtgaa
     3961 ggcaatgcag gggctcctca aagacggtaa tcctatccct tccgccatcg ccgctaactc
     4021 aggtatctac agcgctggag gaggtggaag cggaggagga ggaagcggag gaggaggtag
     4081 cggacctaag aaaaagagga aggtggcggc cgctggatcc attttcaaac cagaagaact
     4141 acgacaggca ctgatgccaa ctttggaggc actttaccgt caggatccag aatcccttcc
     4201 ctttcgtcaa cctgtggacc ctcagctttt aggaatccct gattactttg atattgtgaa
     4261 gagccccatg gatctttcta ccattaagag gaagttagac actggacagt atcaggagcc
     4321 ctggcagtat gtcgatgata tttggcttat gttcaataat gcctggttat ataaccggaa
     4381 aacatcacgg gtatacaaat actgctccaa gctctctgag gtctttgaac aagaaattga
     4441 cccagtgatg caaagccttg gatactgttg tggcagaaag ttggagttct ctccacagac
     4501 actgtgttgc tacggcaaac agttgtgcac aatacctcgt gatgccactt attacagtta
     4561 ccagaacagg tatcatttct gtgagaagtg tttcaatgag atccaagggg agagcgtttc
     4621 tttgggggat gacccttccc agcctcaaac tacaataaat aaagaacaat tttccaagag
     4681 aaaaaatgac acactggatc ctgaactgtt tgttgaatgt acagagtgcg gaagaaagat
     4741 gcatcagatc tgtgtccttc accatgagat catctggcct gctggattcg tctgtgatgg
     4801 ctgtttaaag aaaagtgcac gaactaggaa agaaaataag ttttctgcta aaaggttgcc
     4861 atctaccaga cttggcacct ttctagagaa tcgtgtgaat gactttctga ggcgacagaa
     4921 tcaccctgag tcaggagagg tcactgttag agtagttcat gcttctgaca aaaccgtgga
     4981 agtaaaacca ggcatgaaag caaggtttgt ggacagtgga gagatggcag aatcctttcc
     5041 ataccgaacc aaagccctct ttgcctttga agaaattgat ggtgttgacc tgtgcttctt
     5101 tggcatgcat gttcaagagt atggctctga ctgccctcca cccaaccaga ggagagtata
     5161 catatcttac ctcgatagtg ttcatttctt ccgtcctaaa tgcttgagga ctgcagtcta
     5221 tcatgaaatc ctaattggat atttagaata tgtcaagaaa ttaggttaca caacagggca
     5281 tatttgggca tgtccaccaa gtgagggaga tgattatatc ttccattgcc atcctcctga
     5341 ccagaagata cccaagccca agcgactgca ggaatggtac aaaaaaatgc ttgacaaggc
     5401 tgtatcagag cgtattgtcc atgactacaa ggatattttt aaacaagcta ctgaagatag
     5461 attaacaagt gcaaaggaat tgccttattt cgagggtgat ttctggccca atgttctgga
     5521 agaaagcatt aaggaactgg aacaggagga agaagagaga aaacgagagg aaaacaccag
     5581 caatgaaagc acagatgtga ccaagggaga cagcaaaaat gctaaaaaga agaataataa
     5641 gaaaaccagc aaaaataaga gcagcctgag taggggcaac aagaagaaac ccgggatgcc
     5701 caatgtatct aacgacctct cacagaaact atatgccacc atggagaagc ataaagaggt
     5761 cttctttgtg atccgcctca ttgctggccc tgctgccaac tccctgcctc ccattgttga
     5821 tcctgatcct ctcatcccct gcgatctgat ggatggtcgg gatgcgtttc tcacgctggc
     5881 aagggacaag cacctggagt tctcttcact ccgaagagcc cagtggtcca ccatgtgcat
     5941 gctggtggag ctgcacacgc agagccagga ctacccgtac gacgttccgg actacgcttc
     6001 ttgtacaggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa
     6061 tcctggccca accatgaaaa agcctgaact caccgctacc tctgtcgaga agtttctgat
     6121 cgaaaagttc gacagcgtct ccgacctgat gcagctctcc gagggcgaag aatctcgggc
     6181 tttcagcttc gatgtgggag ggcgtggata tgtcctgcgg gtgaatagct gcgccgatgg
     6241 tttctacaaa gatcgctatg tttatcggca ctttgcatcc gccgctctcc ctattcccga
     6301 agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     6361 gggtgtcacc ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc agcccgtcgc
     6421 cgaggccatg gatgccatcg ctgccgccga tcttagccag accagcgggt tcggcccatt
     6481 cggacctcaa ggaatcggtc aatacactac atggcgcgat ttcatctgcg ctattgctga
     6541 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcct ccgtcgccca
     6601 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6661 cgccgatttc ggctccaaca atgtcctgac cgacaatggc cgcataacag ccgtcattga
     6721 ctggagcgag gccatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     6781 gccctggttg gcttgtatgg agcagcagac ccgctacttc gagcggaggc atcccgagct
     6841 tgcaggatct cctcggctcc gggcttatat gctccgcatt ggtcttgacc aactctatca
     6901 gagcttggtt gacggcaatt tcgatgatgc agcttgggct cagggtcgct gcgacgcaat
     6961 cgtccggtcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgctgccgt
     7021 ctggaccgat ggctgtgtgg aagtgctcgc cgatagtgga aacagacgcc ccagcactcg
     7081 tcctagggca aaggatctgc agtaatgaga attcgatatc aagcttatcg gtaatcaacc
     7141 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7201 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7261 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7321 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7381 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7441 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7501 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7561 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7621 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7681 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     7741 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7801 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     7861 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7921 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     7981 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     8041 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     8101 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     8161 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     8221 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     8281 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8341 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8401 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     8461 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8521 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8581 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8641 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     8701 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     8761 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8821 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8881 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8941 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9001 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9061 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9121 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     9181 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9241 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9301 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9361 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9421 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     9481 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     9541 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     9601 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     9661 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     9721 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     9781 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     9841 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     9901 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     9961 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
    10021 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    10081 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    10141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    10261 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10321 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10381 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10441 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10501 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    10621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    10681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    10741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    10801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    10861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    10921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    10981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11101 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    11581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    11641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    11701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    11761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    11821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    11881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    11941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12421 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    12481 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    12541 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
    12601 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    12661 gggccagata tacgcgtt
//
