LOCUS       sequence-334155-        9892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDL14 is conditionally replicating plasmid and expresses gam, bet 
            and mutSK622A genes under control of PrhaB promoter for 
            oligo-mediated E. coli chromosome engineering in MMR independent 
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Bubnov DM, Yuzbashev TV, Vybornaya TV, Netrusov AI, Sineoky SP
  TITLE     Development of new versatile plasmid-based systems for 
            lambdaRed-mediated Escherichia coli genome engineering.
  JOURNAL   J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001.
  PUBMED    29885886
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001."
COMMENT     Sequence Label: pDL14
FEATURES             Location/Qualifiers
     source          1..9892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             153..569
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             575..1360
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     RBS             1567..1578
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2158..2169
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      4214..4300
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4392..4419
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        complement(join(4454..4460,4461..4478,4479..4484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4499..4520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(4533..5615)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5577..5596
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5616..5693)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(5740..5754,5755..5760,5761..5777,5778..5784)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5760..5776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5792..5808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5827..5838
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5857..5874
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5946..5963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6117..6705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6197..6216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6876..7667,7668..7736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7499..7518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7737..7841)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7867..7961
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(7989..8837)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(8911..9747)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
       61 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
      121 tgaaattcgg atcccgtcga cgcttataaa aaatggatat taatactgaa actgagatca
      181 agcaaaagca ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc
      241 gatattttca cagctatttc aggagttcag ccatgaacgc ttattacatt caggatcgtc
      301 ttgaggctca gagctgggcg cgtcactacc agcagctcgc ccgtgaagag aaagaggcag
      361 aactggcaga cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg
      421 atcatttgca acgccacggg gccagcaaaa aatccattac ccgtgcgttt gatgacgatg
      481 ttgagtttca ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc
      541 accaggttga tattgattca gaggtataaa acgaatgagt actgcactcg caacgctggc
      601 tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac
      661 tcttcgccag acggcattta aaggtgatgc cagcgatgcg cagttcatcg cattactgat
      721 cgttgccaac cagtacggcc ttaatccgtg gacgaaagaa atttacgcct ttcctgataa
      781 gcagaatggc atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa
      841 ccagcagttt gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta
      901 ccgcaaggac cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga
      961 accattcaaa actcgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg
     1021 gatgttacgt cataaagcca tgattcagtg tgcccgtctg gccttcggat ttgctggtat
     1081 ctatgacaag gatgaagccg agcgcattgt cgaaaatact gcatacactg cagaacgtca
     1141 gccggaacgc gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct
     1201 gatcgccctg gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg
     1261 ccgcgacatt cgtgcatcgt cagaactgac acaggccgaa gcagtaaaag ctcttggatt
     1321 cctgaaacag aaagccgcag agcagaaggt ggcagcatga caccggacat tatcctgcag
     1381 cgtaccggga tcgatgtgag agctgtcgaa cagggggaag atgcgtggca caaattacgg
     1441 ctcggcgtca tcaccgcttc agaagttcac aacgtgatag caaaaccccg ctccggaaag
     1501 aagtggcctg acatgaaaat gtcctacttc cacaccctgc ttgctgaggt ttgcaccgct
     1561 agaattaaag aggagaaatt aactatgaat gagtcatttg ataaggactt ctccaaccac
     1621 accccgatga tgcagcagta tctcaagctg aaagcccagc atccggagat cctgctcttt
     1681 tatcgtatgg gagactttta cgagctgttt tatgacgacg caaagcgtgc gtcgcagttg
     1741 ctcgatattt cgctgaccaa acgcggcgca tcggctggcg aacctatccc tatggcgggt
     1801 atcccgcacc acgccgtaga aaactacctc gcgaaactgg tcaatcaggg cgaatccgtc
     1861 gctatttgcg aacagattgg cgatccggcc accagcaaag gtcccgttga acgtaaggtc
     1921 gtgcgtatcg ttacgcctgg cactatcagc gatgaagccc tgttacagga gcgtcaggat
     1981 aacctgctgg cggctatctg gcaggatggt aaaggttacg gttacgccac gctggatatc
     2041 agctccgggc gctttcgtct gagcgaaccc gccgaccgtg aaacgatggc cgcagagctg
     2101 cagcgcacca atcccgccga actgttgtat gccgaagatt ttgctgaaat ggcgttaata
     2161 gagggacgcc ggggtctgcg ccgtcgcccg ttgtgggagt ttgagattga taccgcccgc
     2221 cagcagttaa atctgcagtt cggtacgcgg gatctggtcg gtttcggcgt cgaaaatgcc
     2281 tcgcgtggat tatgtgcggc aggctgcctg ttacagtacg taaaagatac ccagcgcacc
     2341 tccctgccgc atattcgttc cattacgatg gaacgccagc aggacagcat cattatggat
     2401 gccgcgaccc gccgcaatct ggaaattacc cagaacctgg ccggtggtgt cgaaaatacc
     2461 ctcgccgcgg tgcttgactg taccgtgacg ccaatgggta gccgaatgct taaacgctgg
     2521 ctgcatatgc cggtacgaaa taccgacata ttacgtgaac gccagcagac catcggcgcc
     2581 ttgcaggaca ccgtcagcga actgcaaccg gtgctgcgtc aggtcggcga tttggagcgt
     2641 atccttgcgc gtctggcgct gcgcaccgcg cgtccgcgcg atctcgcccg aatgcgtcac
     2701 gccttccagc agctgccgga attgcacgct cagcttgaaa ccgttgacag cgcgccggta
     2761 caggcgttgc gtaaaaaaat gggcgatttc gccgagctgc gcgacctcct ggaacgcgcc
     2821 attattgacg cgccgccggt actggtccgc gacggcggcg ttattgcgcc cggctaccat
     2881 gaagagctgg acgaatggcg cgcgctggcg gacggcgcca ccgattatct cgatcgtctg
     2941 gaaattcgcg agcgcgagcg taccgggctg gatacgctga aagtcggcta taacgcggta
     3001 catggttatt acattcagat tagccgtggt caaagccatc tggcgcccat caattatgtg
     3061 cgtcgccaga cgctgaaaaa tgccgaacgc tacattattc ctgagcttaa agagtacgaa
     3121 gataaagttc tgacctcgaa aggcaaagcg ctggcgctgg aaaaacagct atatgacgaa
     3181 ttgtttgatc tgctgttgcc gcatctggcc gatttacagc agagcgccaa cgcgctggcg
     3241 gagctggatg tgctggttaa cctggccgaa cgcgcctgga cgctgaatta cacctgcccg
     3301 acatttaccg ataaacccgg tatccgtatt accgaaggtc gccacccggt ggttgaacag
     3361 gtactgaatg agccgtttat cgctaacccg ctcaatttat cgccgcagcg ccggatgttg
     3421 atcattaccg gccccaatat gggcggtgca agtacctata tgcgacaaac ggcattgatt
     3481 gccctgctgg cctatatcgg cagttacgtt ccggcgcaaa acgtggaaat cggccccatc
     3541 gaccgtattt ttacccgcgt cggcgcagcg gacgacctgg cctccgggcg ttcgaccttt
     3601 atggtggaga tgaccgaaac cgcgaacatt ctgcataatg ccacggaaaa cagtctggta
     3661 ttgatggatg aaatcgggcg cggtacctcc acgtatgacg ggctgtcgtt ggcctgggcc
     3721 tgcgcggaga atctcgcgaa taagattaaa gcgttaacgc tgttcgctac ccactacttc
     3781 gagctgaccc aattaccgga gaaaatggaa ggcgtggcga acgtccacct ggatgcgctg
     3841 gaacacggcg atactatcgc gtttatgcac agcgtgcagg acggcgcggc aagtaagagc
     3901 tatggcctgg cggtcgcggc gcttgcgggc gtccctaaag aagtcatcaa acgcgcgcgc
     3961 cagaaactcc gcgagctgga aagcatctcg cccaatgcgg cggcaacgca ggtggacggc
     4021 acgcaaatgt cgttgcttgc cgccccggag gagacatcgc ctgccgttga agcgctggag
     4081 aatctcgatc cggactccct gacgccacgc caggcgctgg agtggatcta tcggctgaaa
     4141 agtctggtgt aaggatcgtg gcagacaagc ccgtcagggc gcgtccatgc gagagtaggg
     4201 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     4261 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     4321 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     4381 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc
     4441 tgccatggcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4501 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4561 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4621 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     4681 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     4741 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     4801 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     4861 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     4921 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     4981 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     5041 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     5101 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     5161 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     5221 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     5281 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     5341 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     5401 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     5461 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     5521 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     5581 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     5641 tccgggcgct atcatgccat accgcgaaag gttttgcacc attcgatggt gtcaacgtaa
     5701 atgcatgccg ctaggccctt tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt
     5761 tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa
     5821 ttcattaaag aggagaaatt aactatgaga ggatcgcatc accatcacca tcacggatct
     5881 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     5941 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6001 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6061 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6301 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6421 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6481 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6541 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6601 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6661 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6721 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6781 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6961 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7021 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7081 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7141 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7201 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7261 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7321 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7381 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7441 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7501 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7561 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7621 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7681 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7741 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7801 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7861 ccacctgact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     7921 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tgacgtccgt cggcatcggc
     7981 atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt catcccggtt
     8041 tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc ggtcgaaata
     8101 tcactgatta acaggcggct atgctggaga agatattgcg catgacacac tctgacctgt
     8161 cgcagatatt gattgatggt cattccagtc tgctggcgaa attgctgacg caaaacgcgc
     8221 tcactgcacg atgcctcatc acaaaattta tccagcgcaa agggactttt caggctagcc
     8281 gccagccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg caacgaatca
     8341 ctggtgtaac gatggcgatt cagcaacatc accaactgcc cgaacagcaa ctcagccatt
     8401 tcgttagcaa acggcacatg ctgactactt tcatgctcaa gctgaccgat aacctgccgc
     8461 gcctgcgcca tccccatgct acctaagcgc cagtgtggtt gccctgcgct ggcgttaaat
     8521 cccggaatcg ccccctgcca gtcaagattc agcttcagac gctccgggca ataaataata
     8581 ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc atgaatgtaa
     8641 aagagatcgc cacgggtaat gcgataaggg cgatcgttga gtacatgcag gccattaccg
     8701 cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cagcaaagac atcttgcgga
     8761 taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt
     8821 tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt attcgcaata
     8881 tggcgtacaa atacgttgag aagattcgcg ttattgcaga aagccatccc gtccctggcg
     8941 aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc
     9001 gctgaatcca cagcgatagg cgatgtcagt aacgctggcc tcgctgtggc gtagcagatg
     9061 tcgggctttc atcagtcgca ggcggttcag gtatcgctga ggcgtcagtc ccgtttgctg
     9121 cttaagctgc cgatgtagcg tacgcagtga aagagaaaat tgatccgcca cggcatccca
     9181 attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga gacgtgatgc
     9241 gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta attgcataaa
     9301 caagatctcg cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg
     9361 tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata
     9421 ctgcccatcc agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc
     9481 cggcgagcga tacagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg
     9541 atcatgatcg cgtacgaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa
     9601 cacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc
     9661 aggaaaatcc gcctgcggga gccggggttc tatcgccacg gacgcgttac cagacggaaa
     9721 aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat
     9781 agtaatcacg aggtcaggtt cttaccttaa attttcgacg gaaaaccacg taaaaaacgt
     9841 cgatttttca agatacagcg tgaattttca ggaaatgcgg tgagcatcac at
//
