LOCUS       sequence-334158-       15104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Equalizer-L on an episomal vector. This plasmid encodes eGFP to 
            report circuit output levels. This plasmid also encodes a separate 
            mCherry expression cassette to monitor plasmid dosage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15104)
  AUTHORS   Yang J, Lee J, Land MA, Lai S, Igoshin OA, St-Pierre F
  TITLE     A synthetic circuit for buffering gene dosage variation between 
            individual mammalian cells.
  JOURNAL   Nat Commun. 2021 Jul 5;12(1):4132. doi: 10.1038/s41467-021-23889-0.
  PUBMED    34226556
REFERENCE   2  (bases 1 to 15104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-5"; volume: "12(1):4132. doi"; pages: " 
            10.1038/s41467-021-23889-0"
COMMENT     Sequence Label: Equalizer-L episome
FEATURES             Location/Qualifiers
     source          1..15104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        177..322
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             365..1402
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1400..1419)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1444..1492
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2216..2595
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2596..2799
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2750..2770
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2795..2813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          3022..3594
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3628..3637
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4312..4368
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4375..4377,4378..4380,4381..5094)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4375..4377,4378..4380,4381..5091)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4420..4441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4681..4700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5028..5049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           5300..5314
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5342..5470
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     polyA_signal    5518..5652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5542..5561)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5596..5615
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5795..5817)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6066..7244
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6297..7235
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7192..7212
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7283..7292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7289..7999
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7434..7452)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7702..7721
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8091..8110)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8156..8211
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8210..8229)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             complement(10680..12605)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGXGXGGGXGXGGGXGGXGGXGXGGGXGAGGGAGGAGAGGGA
                     GGAGGXGAGGGXGAGGGXGGXGAGGGAGGAGGAGAGGGAGAGGAGGAGGXGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGXGGXGAGGAGGAGAGGGAGGAGAG
                     GGAGGXGAGGXGGXGXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(13040..13058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13126..13230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13231..13299,13300..14091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13449..13468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14262..14850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14751..14770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
       61 cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt catccccgtg gcccgttgct
      121 cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga
      181 cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
      241 ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
      301 gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcccg gggggcaatg
      361 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
      421 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
      481 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
      541 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
      601 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
      661 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
      721 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
      781 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
      841 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
      901 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
      961 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     1021 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     1081 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     1141 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     1201 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     1261 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     1321 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg
     1381 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     1441 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     1501 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     1561 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     1621 ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga
     1681 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
     1741 ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca
     1801 tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg
     1861 acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt
     1921 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     1981 ggtaggtatg gaagatctcc cgatccccta tggtgcactc tcagtacaat ctactctgat
     2041 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     2101 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     2161 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     2221 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     2281 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     2341 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     2401 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2461 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2521 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2581 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2641 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2701 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2761 ggtaggcgtg tacggtggga ggtctatata agcatcccta tcagtgatag agatcagatc
     2821 tccctatcag tgatagagag ctgttttgac ctccatagaa gacaccggga ccgatccagc
     2881 ctccggactc tagcgtttaa acttaagctg ggtacccggg gatcctctag ggcctctgag
     2941 ctattccaga agtagtgaag aggctttttt ggaggcctag gcttttgcaa aaagctccgg
     3001 atcgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
     3061 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     3121 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     3181 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     3241 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     3301 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     3361 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     3421 tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt catcatcctg
     3481 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     3541 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct acaggtcctg
     3601 caccgcttga agtctttaat taaaggcgcc accatggagt ctagactgga caagagcaaa
     3661 gtcataaact ctgctctgga attactcaat gaagtcggta tcgaaggcct gacgacaagg
     3721 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     3781 cgggccctgc tcgatgccct ggcaatcgag atgctggaca ggcatcatac ccacttctgc
     3841 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc attccgctgt
     3901 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     3961 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     4021 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggat
     4081 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     4141 cttctgagac aagcaattga gctgttcgac catcagggag ccgaacctgc cttccttttc
     4201 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggcca
     4261 aaaaagaaga gaaagggtga cggtgctggt ttaattaaca tgggaagcgg agctactaac
     4321 ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctgc tagcatggtg
     4381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4441 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4561 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4801 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4861 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4921 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4981 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5041 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtagccgctt
     5101 gaagtcttta attaaaaagc ttgtgagtat gtgctcgctt cggcagcaca tatactatgt
     5161 cgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa cacttgctgg
     5221 gattacttct tcaggttaac ccaacagaag gctcgagtgc tgttgacagt gagcgccgct
     5281 tgaagtcttt aattaaatag tgaagccaca gatgtattta attaaagact tcaagcggtg
     5341 cctactgcct cggagaattc aaggggctac tttaggagca attatcttgt ttactaaaac
     5401 tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat ggtataaatt
     5461 aaatcacttt tttcaattgt ttcctttttt ttcctcagct cgaggccggc aaggccggat
     5521 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     5581 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     5641 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     5701 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     5761 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5821 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5881 cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc
     5941 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     6001 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gaaggagtgg
     6061 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     6121 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     6181 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     6241 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     6301 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     6361 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     6421 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     6481 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     6541 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     6601 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     6661 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     6721 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     6781 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     6841 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     6901 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     6961 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     7021 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     7081 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     7141 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     7201 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggtacc ggatcctcta
     7261 gagtcgactc cggacgtcga cggccaccat ggtgagcaag ggcgaggagg ataacatggc
     7321 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     7381 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     7441 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     7501 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     7561 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     7621 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     7681 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     7741 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     7801 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     7861 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     7921 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     7981 ggacgagctg tacaagtaat gagcggccgc ttcactcctc aggtgcaggc tgcctatcag
     8041 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     8101 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     8161 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     8221 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     8281 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     8341 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     8401 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     8461 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     8521 cttctccatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     8581 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     8641 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     8701 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     8761 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     8821 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     8881 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     8941 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     9001 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     9061 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     9121 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     9181 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     9241 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     9301 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     9361 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     9421 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     9481 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     9541 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     9601 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     9661 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     9721 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     9781 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     9841 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     9901 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     9961 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc
    10021 atatacagca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
    10081 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
    10141 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
    10201 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
    10261 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
    10321 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
    10381 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
    10441 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
    10501 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
    10561 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
    10621 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
    10681 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
    10741 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
    10801 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
    10861 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
    10921 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
    10981 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    11041 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    11101 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
    11161 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
    11221 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
    11281 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
    11341 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
    11401 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
    11461 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
    11521 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
    11581 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
    11641 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
    11701 cctcctgccc ctcctsctcc tscccctcct scccctcctg ctcctscccc tcctgcccct
    11761 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
    11821 cctscccctc ctscccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
    11881 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
    11941 cctcctgctc ctgcccctcc tgctcctscc cctcctgccc ctcctgcccc tcctgctcct
    12001 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctscccct
    12061 cctscccctc ctcctgctcc tscccctcct cctgctccts cccctcctgc ccctcctgcc
    12121 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctscccctcc tcctsctcct
    12181 scccctccts cccctcctsc ccctcctcct sctcctsccc ctcctcctsc tcctscccct
    12241 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
    12301 cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg
    12361 cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg tcttggccct
    12421 gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt
    12481 cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt
    12541 ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca
    12601 gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata
    12661 cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc
    12721 gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc
    12781 accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc
    12841 ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc
    12901 tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga
    12961 agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag
    13021 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    13081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    13141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    13201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    13261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    13321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    13381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    13441 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
    13501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    13561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    13621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    13681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    13741 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
    13801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    13861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    13921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    13981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    14041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    14101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    14161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    14221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    14281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    14341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    14401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    14461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    14521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    14581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    14641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    14701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    14761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    14821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    14881 ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
    14941 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
    15001 gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga
    15061 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatg
//
