LOCUS       sequence-334174-       21241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR dCas9 directly fused to a catalytic inactive version of 
            bacterial DNA methyltransferase MQ1 to target to the FWA locus with 
            three guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Ghoshal B, Picard CL, Vong B, Feng S, Jacobsen SE
  TITLE     CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by
            a bacterial CG-specific DNA methyltransferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2125016118. doi: 
            10.1073/pnas.2125016118.
  PUBMED    34074795
REFERENCE   2  (bases 1 to 21241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-8"; volume: "118(23):e2125016118. 
            doi"; pages: " 10.1073/pnas.2125016118"
COMMENT     Sequence Label: pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
FEATURES             Location/Qualifiers
     source          1..21241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..27
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    117..291
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(331..1356)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1424..2101)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_RNA        complement(2186..2261)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(2743..2818)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(3305..3380)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(4131..4181)
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     CDS             complement(4209..4370)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     protein_bind    7081..7105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7124..7179
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             7193..7258
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7265..7285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7295..7321,7322..7324,7325..9808,9809..9814,9815..9880,
                     9881..9883,9884..11398)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(7295..7321,7322..7324,7325..9811,9812..9814,
                     9815..11398)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7295..11398
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11411..11431
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      12626..13333
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(13366..13384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(13367..13384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(13408..13425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13408..13424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13417..13439)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    13627..13651
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14951..15580
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             16014..17081
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      17147..17341
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(17577..17599)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17685..17825
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17740..17759
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17840..17857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18011..18599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18091..18110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(20023..20817)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
       61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctctaat tcgggggatc
      121 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
      181 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
      241 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt
      301 gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
      361 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
      421 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
      481 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
      541 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
      601 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
      661 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
      721 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
      781 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
      841 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
      901 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
      961 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg
     1021 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     1081 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg
     1141 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     1201 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     1261 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     1321 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc
     1381 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca
     1441 aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc
     1501 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     1561 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     1621 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     1681 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     1741 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     1801 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc
     1861 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     1921 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     1981 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     2041 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     2101 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     2161 gtgctccacc atggtaccaa aaaaagcacc gactcggtgc cactttttca agttgataac
     2221 ggactagcct tattttaact tgctatttct agctctaaaa cctctttatc ccattcaaca
     2281 tcaatcacta cttcgactct agctgtatat aaactcagct tcgttttctt atctaagcga
     2341 tgtgggactt ttgaagattg ttttcaactt aaatgggcct atataagaaa tactattgtt
     2401 ctttcccata taaatgggcc tgcttctctt ctttcagatt cccaggggcc ttttgaagat
     2461 tatcttcata tcttaagaat gaagatgttt tattcaatca aattcttgaa ggttcgatgc
     2521 ctaatcattc taatcctggg acaaactatg aaacaagata caaaaactcc gaatggaaag
     2581 ttaaaaagaa gaaaacgaaa gctacggttc aagaaaatgt aagctgataa acaaaaaaaa
     2641 actgtatgaa cgaagaagaa gaaaaaaagc taagaagaaa tgatgtattg tgcggaaggc
     2701 aagtcgagtt tccgttgttc aacgaaagct tgtttaaaaa aagcaccgac tcggtgccac
     2761 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaactt
     2821 caacattcat acgagcgcca atcactactt cgactctagc tgtatataaa ctcagcttcg
     2881 ttttcttatc taagcgatgt gggacttttg aagattgttt tcaacttaaa tgggcctata
     2941 taagaaatac tattgttctt tcccatataa atgggcctgc ttctcttctt tcagattccc
     3001 aggggccttt tgaagattat cttcatatct taagaatgaa gatgttttat tcaatcaaat
     3061 tcttgaaggt tcgatgccta atcattctaa tcctgggaca aactatgaaa caagatacaa
     3121 aaactccgaa tggaaagtta aaaagaagaa aacgaaagct acggttcaag aaaatgtaag
     3181 ctgataaaca aaaaaaaact gtatgaacga agaagaagaa aaaaagctaa gaagaaatga
     3241 tgtattgtgc ggaaggcaag tcgagtttcc gttgttcaac gaaagctttc ctaaaccaaa
     3301 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
     3361 gctatttcta gctctaaaac aagcccatac atctttccgt caatcactac ttcgactcta
     3421 gctgtatata aactcagctt cgttttctta tctaagcgat gtgggacttt tgaagattgt
     3481 tttcaactta aatgggccta tataagaaat actattgttc tttcccatat aaatgggcct
     3541 gcttctcttc tttcagattc ccaggggcct tttgaagatt atcttcatat cttaagaatg
     3601 aagatgtttt attcaatcaa attcttgaag gttcgatgcc taatcattct aatcctggga
     3661 caaactatga aacaagatac aaaaactccg aatggaaagt taaaaagaag aaaacgaaag
     3721 ctacggttca agaaaatgta agctgataaa caaaaaaaaa ctgtatgaac gaagaagaag
     3781 aaaaaaagct aagaagaaat gatgtattgt gcggaaggca agtcgagttt ccgttgttca
     3841 acgaaagctt cgtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     3901 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     3961 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     4021 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     4081 ccaaatgttt gaacgatcgg ggaaattcga gctcaaactt acggccatgg cgtaggcggc
     4141 cttttccgtt gtgatcttcg cggtctcctc cttgtttttg gactagtacc accaccgcta
     4201 ccaccacctt cggttaccgt gaaggttttg gtagcgtcgt cgtaggtcca ttcaccgtca
     4261 acaccgttgt cgttagcgta ctgtttgaaa actttttccg cggtagcagc gtcaacagct
     4321 tcggtggtgg tttcaccttt cagggtttta ccgttcagga taagcttgta ctcttcggtc
     4381 cgagatcctc ctcccgtact cagggttttc acagccaagg ggagaccaaa gtgtgcaggc
     4441 tgttgaatcc ctctatggtt taaagcaagc atcaaggcag tggaacatca agcttaccac
     4501 tgtccttatg caggctggtt ttatgcaaag tgcttatgat cactccttgt ttaccaaaag
     4561 agaggggact gaccttgtca taatcctgat ttatgtggat gatctgttaa taacaggcag
     4621 cagcaatgtt ttgatttcag aagcaaaggc aaccctgcat cagcatttca agatgaaaga
     4681 tttgggagaa ctaaaatact tcttaggcat tgaggtgctg agatcagaaa agggaatcct
     4741 actgaaccag aggaagtatg cactggaact gatatcaggt gttggtctgg gaggctgcag
     4801 accagtgtca accccaatgg aacagaacca aaggctgact actgtagaat atgacaagca
     4861 cttaggaaag actgatgatg cagagttaga ggatgtgggt tcctatcaga gactggtggg
     4921 aaagcttctc tacttgacaa tcacaaggcc agatatcagc tttgcagtgc aggtgctgag
     4981 tcaattcatg cagcaaccca aacagtcaca cctggaagca gcattgaggg ttgtgaagta
     5041 cataaaaggt tctccaggtg ttaggaacgc ggccgcatgc atatgagtct agctcaacag
     5101 agcttttaac ccaaattggt acaatagaat acaactttag atcataattc tcaaaagaaa
     5161 gagattcctt agctattcta tctgccactc catttccttc tcggcttgta tgcacaagca
     5221 taaaatcctc aaacttgcta agtagatact ttatgtcttg gataattgga ttgagacttg
     5281 acaagcataa ctttcatgta accaaagaca caagttgctg agaatccacc tcaaaaatga
     5341 tcttcctata attgaatcgg gataatgaca gcacagccca tctaagagcc tccacttcta
     5401 cttccagcac gcttcttact tttaccacag ctcttgcacc taaccataac accttccctg
     5461 tatgatcgcg aagcacccac cctaagccac attttaatcc ttctgttggc catgccccat
     5521 caaagttgca cttaacccaa gattgtggtg gagcttccca tgtttctcgt ctgtcccgac
     5581 ggtgttgtgg ttggtgcttt ccttacattc tgagcctctt tccttctaat ccactcatct
     5641 gcatcttctt gtgtccttac taatacctca ttggttccaa attccctccc tttaagcacc
     5701 agctcgtttc tgttcttcca cagcctccca agtatccaag ggactaaagc ctccacattc
     5761 ttcagatcag gatattcttg tttaagatgt tgaactctat ggaggtttgt atgaactgat
     5821 gatctaggac cggataagtt cccttcttca tagcgaactt attcaaagaa tgttttgtgt
     5881 atcattcttg ttacattgtt attaatgaaa aaatattatt ggtcattgga ctgaacacga
     5941 gtgttaaata tggaccaggc cccaaataag atccattgat atatgaatta aataacaaga
     6001 ataaatcgag tcaccaaacc acttgccttt tttaacgaga cttgttcacc aacttgatac
     6061 aaaagtcatt atcctatgca aatcaataat catacaaaaa tatccaataa cactaaaaaa
     6121 ttaaaagaaa tggataattt cacaatatgt tatacgataa agaagttact tttccaagaa
     6181 attcactgat tttataagcc cacttgcatt agataaatgg caaaaaaaaa caaaaaggaa
     6241 aagaaataaa gcacgaagaa ttctagaaaa tacgaaatac gcttcaatgc agtgggaccc
     6301 acggttcaat tattgccaat tttcagctcc accgtatatt taaaaaataa aacgataatg
     6361 ctaaaaaaat ataaatcgta acgatcgtta aatctcaacg gctggatctt atgacgaccg
     6421 ttagaaattg tggttgtcga cgagtcagta ataaacggcg tcaaagtggt tgcagccggc
     6481 acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc taaaaataag
     6541 gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt cattttatta
     6601 ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt cttttcttct
     6661 tcttcttcta taaaacaata cccaaagagc tcttcttctt cacaattcag atttcaattt
     6721 ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta aatttctgtg
     6781 ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt cgtatatgtt
     6841 ctttggttta gattctgtta atcttagatc gaagacgatt ttctgggttt gatcgttaga
     6901 tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc aaataatttg
     6961 agttttgtcg aataattact cttcgatttg tgatttctat ctagatctgg tgttagtttc
     7021 tagtttgtgc gatcgaattt gtcgattaat ctgagttttt ctgattaaca gggatcatca
     7081 acaagtttgt acaaaaaagc aggctcttta aagtattttt acaacaatta ccaacaacaa
     7141 caaacaacaa acaacattac aattactatt tacaattaca aaaaaagtta tggactacaa
     7201 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat
     7261 ggcccccaag aagaagagga aggtgggccg cggaatggac aagaagtact ccattgggct
     7321 cgccatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag
     7381 caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg
     7441 cgccctcctg ttcgactccg gggaaaccgc cgaagccacg cggctcaaaa gaacagcacg
     7501 gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga
     7561 gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga
     7621 ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca
     7681 tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc
     7741 tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct
     7801 catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt
     7861 tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa
     7921 agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct
     7981 ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac
     8041 ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga
     8101 cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct
     8161 ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa
     8221 cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca
     8281 ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga
     8341 aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca
     8401 ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct
     8461 gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag
     8521 catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt
     8581 ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc
     8641 ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc
     8701 agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca
     8761 gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc
     8821 taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata
     8881 cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt
     8941 ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt
     9001 caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc
     9061 atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa
     9121 tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag
     9181 ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa
     9241 acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg
     9301 gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc
     9361 caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca
     9421 gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg
     9481 tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa
     9541 agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac
     9601 tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa
     9661 agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga
     9721 gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga
     9781 catcaatcgg ctctccgact acgacgtgga tgccatcgtg ccccagtctt ttctcaaaga
     9841 tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa
     9901 cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc
     9961 caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc
    10021 tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa
    10081 gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact
    10141 gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga
    10201 ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct
    10261 gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt
    10321 ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat
    10381 aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga
    10441 gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac
    10501 aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat
    10561 gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag
    10621 tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa
    10681 gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt
    10741 ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat
    10801 ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga
    10861 ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg
    10921 ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc
    10981 ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc
    11041 cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat
    11101 catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa
    11161 ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat
    11221 tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac
    11281 caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca
    11341 tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag
    11401 cagggctgac cccaagaaga agaggaaggt ggctagggac agcaaagtgg agaacaaaac
    11461 aaagaagctg agagtgttcg aagccttcgc cggcattggc gcccagagaa aggccctgga
    11521 gaaagtgagg aaggacgagt acgagatcgt gggactggcc gagtggtatg tgcccgccat
    11581 cgtcatgtac caggccatcc ataacaactt ccacaccaag ctcgagtaca agtccgtcag
    11641 cagagaggag atgatcgact acctcgagaa caagaccctg tcctggaaca gcaagaaccc
    11701 cgtcagcaac ggatactgga agaggaagaa ggatgacgag ctgaagatca tctacaacgc
    11761 catcaagctg tccgaaaagg agggcaacat tttcgacatc agggacctct acaagaggac
    11821 actgaagaac atcgacctgc tcacctacag cttcccttcc caggacctga gccagcaagg
    11881 catccagaag ggcatgaaga ggggaagcgg caccagatcc ggcctgctct gggagatcga
    11941 aagagccctg gactccaccg agaagaacga cctgcctaag tacctcctca tggagaacgt
    12001 gggagccctg ctgcacaaga agaacgagga ggagctgaac caatggaagc agaagctgga
    12061 gagcctgggc taccagaaca gcatcgaagt cctcaatgct gccgatttcg gatccagcca
    12121 ggccaggagg agagtgttca tgatctccac cctcaatgag ttcgtggaac tgcctaaggg
    12181 cgacaagaag cccaagagca tcaaaaaggt gctgaacaag atcgtgagcg agaaggacat
    12241 cctcaacaac ctgctgaaat acaacctcac cgaattcaag aagaccaagt ccaacatcaa
    12301 caaggccagc ctgatcggct actccaagtt caactccgag ggctatgtgt acgaccccga
    12361 gttcacaggc cccacactga cagctgccgg cgccaactcc aggatcaaga tcaaggacgg
    12421 cagcaacatc aggaagatga acagcgacga gaccttcctg tacatcggct ttgacagcca
    12481 ggacggcaag agagtgaacg aaatcgagtt cctgaccgag aaccagaaga tcttcgtgtg
    12541 tggcaacagc atcagcgtgg aggtgctgga ggccatcatt gacaagatcg gcggccctag
    12601 ctagcgcgcc atgataacta gagtcctgct ttaatgagat atgcgagacg cctatgatcg
    12661 catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc
    12721 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt
    12781 tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat
    12841 attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg
    12901 ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga
    12961 accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag
    13021 gggctagtat ctacgacaca ccgagcggcg aactaataac gttcactgaa gggaactccg
    13081 gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta
    13141 ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct
    13201 gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca
    13261 aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt
    13321 cgaggctcag caggaccggc atgcaagcta gcttactagt gatattctat agtgtcacct
    13381 aaatctctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
    13441 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    13501 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    13561 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc
    13621 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
    13681 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
    13741 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
    13801 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
    13861 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
    13921 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
    13981 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
    14041 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
    14101 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
    14161 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
    14221 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
    14281 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
    14341 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
    14401 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
    14461 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
    14521 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
    14581 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
    14641 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
    14701 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
    14761 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
    14821 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
    14881 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
    14941 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
    15001 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
    15061 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
    15121 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
    15181 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
    15241 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
    15301 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
    15361 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
    15421 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
    15481 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
    15541 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
    15601 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
    15661 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
    15721 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
    15781 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
    15841 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
    15901 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
    15961 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
    16021 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
    16081 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
    16141 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
    16201 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
    16261 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
    16321 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
    16381 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
    16441 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
    16501 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
    16561 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
    16621 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
    16681 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
    16741 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    16801 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    16861 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    16921 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    16981 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    17041 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    17101 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
    17161 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    17221 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    17281 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    17341 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    17401 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    17461 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    17521 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    17581 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    17641 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    17701 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    17761 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    17821 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    17881 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    17941 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    18001 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    18061 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    18121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    18181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    18241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    18301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    18361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    18421 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    18481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    18541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    18601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    18661 ggctgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag atacacatct
    18721 tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa tgtgcgaact
    18781 cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa
    18841 cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc ttaccgaact
    18901 tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt
    18961 gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt
    19021 tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata tccgtgagca
    19081 aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg ttactcttta
    19141 tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc aatttctagc
    19201 cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg atgctggctt
    19261 taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa gtcgagtaag
    19321 aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca gcaacttaca
    19381 tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta aagcaatcca
    19441 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
    19501 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
    19561 tggattctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
    19621 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
    19681 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
    19741 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
    19801 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
    19861 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct
    19921 gcaaccctac tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg
    19981 gatctctcag cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
    20041 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    20101 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    20161 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    20221 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    20281 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    20341 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    20401 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    20461 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    20521 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    20581 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    20641 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    20701 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    20761 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    20821 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    20881 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    20941 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    21001 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    21061 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    21121 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    21181 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    21241 g
//
