LOCUS       sequence-334203-       10331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mnuctdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT(EF1a no GFP) mnuctdTomato
FEATURES             Location/Qualifiers
     source          1..10331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2325..2349
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2458..3888
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     promoter        4009..5194
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4240..5185
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5142..5162
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5279..6304
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5279..6304
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7537..7770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7842..7963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7879..7898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7933..7952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8065..8084
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8349..8367)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8435..8539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8540..8608,8609..9400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8758..8777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9571..10159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10060..10079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatt tcgaacctag ggtttaaacg
     2281 gcgcgccagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
     2341 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     2401 ttcctaccct cgtaaagtcg acccaccatg actgctccaa agaagaagcg taaggtaatg
     2461 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     2521 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2581 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     2641 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     2701 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2761 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     2821 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     2881 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     2941 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     3001 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     3061 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     3121 caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc
     3181 tcctccgagg acaacaacat ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg
     3241 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     3301 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     3361 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
     3421 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     3481 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
     3541 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
     3601 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     3661 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     3721 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     3781 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     3841 ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagtaaac cgtcgacctc
     3901 gacggatcgg gagatctcga ccgagctttg caaagatgga taaagtttta aacagagagg
     3961 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     4021 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4081 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4141 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4201 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     4261 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     4321 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     4381 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     4441 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     4501 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     4561 gtaaatgcgg gccaagatga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     4621 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     4681 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     4741 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     4801 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     4861 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     4921 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     4981 atcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     5041 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     5101 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     5161 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt agcttgacca agcttggtac
     5221 cgagctcgga tccactagta acggccgcca gtgtgctgga attctcgagg tcgccaccat
     5281 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     5341 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     5401 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     5461 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     5521 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     5581 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     5641 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     5701 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     5761 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     5821 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     5881 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     5941 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     6001 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     6061 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     6121 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     6181 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     6241 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     6301 ataggctagc ctagtgccat ttgttcagtg gttcgtaggg ctttccccca ctgtttggct
     6361 ttcagttata tggatgatgt ggtattgggg gccaagtctg tacagcatct tgagtccctt
     6421 tttaccgctg ttaccaattt tcttttgtct ttgggtatac atttaaaccc taacaaaaca
     6481 aagagatggg gttactctct aaattttatg ggttatgtca ttggatgtta tgggtccttg
     6541 ccacaagaac acatcataca aaaaatcaaa gaatgtttta gaaaacttcc tattaacagg
     6601 cctattgatt ggaaagtatg tcaacgaatt gtgggtcttt tgggttttgc tgcccctttt
     6661 acacaatgtg gttatcctgc gttgatgcct ttgtatgcat gtattcaatc taagcaggct
     6721 ttcactttct cgccaactta caaggccttt ctgtgtaaac aatacctgaa cctttacccc
     6781 gttgcccggc aacggccagg tctgtgccaa gtgtttgctg acgcaacccc cactggctgg
     6841 ggcttggtca tgggccatca gcgcatgcgt ggaacctttt cggctcctct gccgatccat
     6901 actgcggaac tcctagccgc ttgttttgct cgcagcaggt ctggagcaaa cattatcggg
     6961 actgataact ctgttgtcct atcccgcaaa tatacatcgt ttccatggct gctaggctgt
     7021 gctgccaact ggatcctgcg cgggacgtcc tttgtttacg tcccgtcggc gctgaatcct
     7081 gcggacgacc cttctcgggg tcgcttggga ctctctcgtc cccttctccg tctgccgttc
     7141 cgaccgacca cggggcgcac ctctctttac gcggactccc cgtctgtgcc ttctcatctg
     7201 ccggaccgtg tgcacttcgc ttcacctctg cacgtcgcat ggagaccacc gtgaacgccc
     7261 accaaatatt gcccaaggtc ttacataaga ggactcttgg actctcagca atgtcaacga
     7321 ccgaccttga ggcatacttc aaagactgtt tgtttaaaga ctgggaggag ttgggggagg
     7381 agattaggtt aaaggtcttt gtactaggag gctgtaggca taaattggtc tgcgcaccag
     7441 caccatggcg caatcactag agcggggtac ctttaagacc aatgacttac aaggcagctg
     7501 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7561 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7621 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7681 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7741 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7801 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7921 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7981 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccggat cccttgagtg
     8101 gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt
     8161 gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca
     8221 ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag
     8281 cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta attcttgaag
     8341 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8401 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8461 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8521 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8581 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8641 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8701 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8761 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     8821 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8881 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8941 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9001 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9061 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9121 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9181 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9241 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9301 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9361 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9421 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9481 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9541 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9601 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9661 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     9721 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9781 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9841 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9901 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9961 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10021 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10081 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10141 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10201 ctggcctttt tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
    10261 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
    10321 gcctcgcgtc g
//
