LOCUS       sequence-334217-       12120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 
            and nsp8 (SARS-CoV-2) in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12120)
  AUTHORS   Bertolin AP, Weissmann F, Zeng J, Posse V, Milligan JC, Canal B, 
            Ulferts R, Wu M, Drury LS, Howell M, Beale R, Diffley JFX
  TITLE     Identifying SARS-CoV-2 antiviral compounds by screening for small 
            molecule inhibitors of nsp12/7/8 RNA-dependent RNA polymerase.
  JOURNAL   Biochem J. 2021 Jul 16;478(13):2425-2443. doi: 10.1042/BCJ20210200.
  PUBMED    34198323
REFERENCE   2  (bases 1 to 12120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2021-07-16- 16"; volume: "478"; issue: "13"; 
            pages: "2425-2443"
COMMENT     Sequence Label: pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)
FEATURES             Location/Qualifiers
     source          1..12120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        303..405
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             406..1065
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(472..491)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1365..1819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1452..1471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1661..1682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1845..1949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1950..2018,2019..2810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2168..2187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2981..3569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3470..3489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3723..3740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3874..4098
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4165..4698)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4887..4892,4893..4909,4910..4915))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5311..5344)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5442..5490
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5585..5676
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             8534..8551
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8558..8623
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    8756..8890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9249..9297
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9392..9483
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9917..10051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    10410..10458
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        10553..10644
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      10688..10697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    11423..11557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  11873..12038
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgaacagga gggacagctg atagaaacag
      121 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
      181 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
      241 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
      301 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      361 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      421 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      481 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      541 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      601 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      661 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      721 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      781 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      841 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      901 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      961 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     1021 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1081 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     1141 ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag
     1201 ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt
     1261 gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg
     1321 atatgatcat ttattctgcc tcccagagcc tgatggcgaa tgggacgcgc cctgtagcgg
     1381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1441 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1501 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1561 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1621 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
     1681 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1741 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1801 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3121 atactattct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3721 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3781 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
     3841 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
     3901 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
     3961 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
     4021 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
     4081 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     4141 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     4201 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     4261 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     4321 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     4381 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     4441 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     4501 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     4561 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     4621 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     4681 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     4741 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     4801 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     4861 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     4921 tttgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     4981 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     5041 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc
     5101 ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     5161 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     5221 gctatagttc tagtggttgg ctacagcttt gtttgtacta tcaacaggtt gaactgctga
     5281 tcaacagatc ctctacgcgg ccgcggtacc ataacttcgt atagcataca ttatacgaag
     5341 ttatctggcc taggttaatt aaacgctcta tggtctaaag tttttatcga ggctaactga
     5401 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     5461 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5521 actctgtcaa gtgaacgctc tatggtctaa agatttaaat cgacctactc cggaatatta
     5581 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
     5641 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
     5701 accatcgggc gcggatccag ccaccatgtc tgctgacgct cagtccttcc tgaaccgtgt
     5761 gtgcggtgtc tctgctgctc gtctgactcc ttgcggtact ggaacctcta ccgacgtggt
     5821 gtaccgtgct ttcgacatct acaacgacaa ggtggccggt ttcgctaagt tcctcaagac
     5881 caactgctgc cgcttccaag agaaggacga ggacgacaac ctgatcgact cctacttcgt
     5941 ggtcaagcgt cacaccttct ccaactacca gcacgaggaa actatctaca acctgctgaa
     6001 ggactgcccc gctgtggcta agcacgactt cttcaagttc cgtatcgacg gcgacatggt
     6061 gccccacatc tctcgtcagc gtctgaccaa gtacaccatg gctgacctgg tgtacgctct
     6121 gcgccacttc gatgagggaa actgcgacac cctgaaagag atcctggtca cctacaactg
     6181 ctgtgacgac gactacttca acaagaagga ttggtacgac ttcgtcgaga accccgacat
     6241 cctgcgtgtg tacgctaacc tgggagagcg tgtgcgtcag gctctgctca agactgtgca
     6301 gttctgcgac gctatgcgta acgctggtat cgtgggagtg ctgaccctgg acaaccagga
     6361 cctgaacggc aattggtatg atttcggcga cttcatccag actacccctg gttctggtgt
     6421 ccccgtggtg gactcttact actccctgct gatgcccatc ctgactctga cccgtgctct
     6481 gaccgctgag tctcacgtgg acaccgacct gaccaagcct tacatcaagt gggacttgct
     6541 gaagtacgat ttcaccgagg aacgcctgaa gctgttcgac cgttacttca agtactggga
     6601 ccagacctac catcctaact gcgtgaactg cctggacgac cgttgcatcc tgcactgcgc
     6661 taacttcaac gtgctgttct ccactgtgtt ccctccaacc tccttcggtc ctctcgtgcg
     6721 caagatcttc gtggacggtg tccctttcgt ggtgtccacc ggttaccact tccgtgaact
     6781 gggtgtcgtg cataaccagg acgtcaacct ccactcctct cgtctgtcct tcaaagagct
     6841 gctggtctac gctgctgacc ccgctatgca tgctgcttcc ggaaacctgc tgctggacaa
     6901 gcgtaccact tgcttctccg tggctgccct gactaacaac gtggccttcc agaccgtgaa
     6961 gcccggcaat ttcaacaagg acttctacga tttcgccgtg tccaagggct tcttcaaaga
     7021 aggctcctcc gtcgagctga agcacttctt cttcgctcaa gacggcaacg ctgctatctc
     7081 cgactacgac tactaccgtt acaacttgcc caccatgtgc gacatccgtc agctgctgtt
     7141 cgtggtcgag gtggtggaca agtacttcga ctgctacgac ggtggttgca tcaacgccaa
     7201 tcaagtgatc gtgaacaacc tggacaagtc cgctggcttc cccttcaaca aatggggcaa
     7261 agctcgtctg tactacgact ccatgtccta cgaggaccag gacgctctgt tcgcttacac
     7321 caagcgtaac gtgatcccca ccatcactca gatgaacctg aaatacgcca tctccgctaa
     7381 gaaccgtgct cgtaccgtgg ctggtgtctc catctgctcc accatgacca accgtcagtt
     7441 ccaccagaag ctgctgaagt caatcgctgc tacccgcggt gctaccgtgg tcatcggtac
     7501 ttccaagttc tacggtggct ggcacaacat gctgaaaacc gtgtactccg acgtggaaaa
     7561 ccctcacctg atgggctggg actaccccaa gtgcgatcgt gctatgccta acatgctgcg
     7621 tatcatggct tccctggtgc tggctcgcaa gcacactact tgctgctccc tgtctcaccg
     7681 tttctaccgt ctggctaacg agtgcgctca ggtgctgtcc gagatggtca tgtgcggtgg
     7741 ttccctgtac gtgaagcctg gtggtacttc ctctggcgac gctaccaccg cttacgctaa
     7801 ctccgtgttc aacatctgcc aggctgtgac cgctaacgtg aacgctctgc tgtccaccga
     7861 cggcaacaag atcgctgaca aatacgtgcg taacctgcag caccgtctgt acgagtgcct
     7921 gtaccgtaac cgtgacgtcg acactgactt cgtgaacgag ttctacgctt acctgcgcaa
     7981 gcacttcagc atgatgatcc tgtccgacga cgctgtcgtg tgcttcaact ccacctacgc
     8041 ttcccaaggc ctggtggctt ccatcaagaa cttcaagtcc gtgctgtact accagaacaa
     8101 cgtgttcatg tccgaggcta agtgctggac cgagactgac ctgactaagg gtccccacga
     8161 gttctgctcc cagcacacca tgctggtcaa gcagggcgac gattacgtgt acctgcctta
     8221 tcctgatcct tctcgtatcc tcggcgctgg ttgcttcgtg gatgacatcg tcaagaccga
     8281 cggaaccctg atgatcgagc gtttcgtgtc cctggctatc gacgcttacc ctctgactaa
     8341 gcaccccaat caagagtacg ctgacgtgtt ccacctgtac ctgcagtaca tccgcaagct
     8401 gcacgacgag ctgaccggac acatgctgga catgtactcc gtgatgctga ccaacgacaa
     8461 cacctctcgt tactgggagc ccgaattcta cgaggctatg tacacccctc acaccgtgct
     8521 gcagggtggc tcccaccatc accaccatca cggttctgac tacaaggacc acgacggcga
     8581 ttacaaggat catgacatcg actacaaaga cgacgatgac aagtaaaagc ttgtcgagaa
     8641 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     8701 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     8761 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8821 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8881 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
     8941 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccattt aaataaatct
     9001 atatctcaat cggggttacg tcgatacccc accgagaccc cattgggacc aatacgcccg
     9061 cgtttcttcc ttttccccac cccaaccccc aagttcgggt gaaggcccag ggctcgcagc
     9121 caacgtcggg gcggcaagcc ctgccatagc cactacgggt taattaaaaa ctatatctca
     9181 atcggggttt ttatcgaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     9241 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
     9301 aacgcggggt tcggtcccag ggctggcact ctgtcaagtg aacgctctat ggtctaaaga
     9361 tttaaatcga cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat
     9421 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
     9481 aatattccgg attattcata ccgtcccacc atcgggcgcg gatccagcca ccatgtccaa
     9541 gatgtccgac gtgaagtgca cctccgtggt gctgctgtct gtgctgcaac agctgcgtgt
     9601 ggaatcctcc agcaagctgt gggctcagtg cgtccaactg cacaacgaca tcctgctggc
     9661 taaggacacc accgaggctt tcgagaagat ggtgtccctg ctgtccgtcc tgctgtctat
     9721 gcaaggtgct gtggacatca acaagctgtg cgaagagatg ctggacaacc gtgctaccct
     9781 gcagtaaaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     9841 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     9901 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9961 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10021 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg
    10081 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat
    10141 cataaccatt taaataaatc tatatctcaa tcggggttac gtcgataccc caccgagacc
    10201 ccattgggac caatacgccc gcgtttcttc cttttcccca ccccaacccc caagttcggg
    10261 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaagc cctgccatag ccactacggg
    10321 ttaattaaaa actggatact attgcacgtt tttatcgagg ctaactgaaa cacggaagga
    10381 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
    10441 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcaagt
    10501 gaacgctcta tggtctaaag atttaaatcg acctactccg gaatattaat agatcatgga
    10561 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt
    10621 tttgtaataa aaaaacctat aaatattctg gattattcat accgtcccac catcgggcgc
    10681 ggatccagcc accatggcta tcgcttccga gttctcctct ctgccctcct acgctgcttt
    10741 cgctaccgct caagaggctt acgagcaggc tgtggctaac ggcgactctg aggtggtgct
    10801 gaagaagctg aaaaagtccc tgaacgtggc caagtccgag ttcgaccgtg acgctgctat
    10861 gcagcgcaag ctcgagaaga tggctgacca ggctatgacc cagatgtaca agcaggctcg
    10921 ttccgaggac aagcgtgcta aagtgacctc cgctatgcag accatgctgt tcaccatgct
    10981 ccgcaagctg gacaacgacg ctctgaacaa catcatcaac aacgctcgtg acggttgcgt
    11041 gcccctgaac atcatccctc tgaccaccgc tgctaagctg atggtggtca tccccgacta
    11101 caacacctac aagaacactt gcgacggcac caccttcacc tacgcttccg ctctgtggga
    11161 gatccagcaa gtggtggacg ctgactccaa gatcgtgcag ctgtccgaga tctccatgga
    11221 caactctccc aacctggctt ggcccctgat cgtgactgct ctgcgtgcta actccgctgt
    11281 gaagctgcag taaaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
    11341 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    11401 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    11461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11521 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag
    11581 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
    11641 aactatcata accatttaaa taaatctata tctcaatcgg ggttacgtcg ataccccacc
    11701 gagaccccat tgggaccaat acgcccgcgt ttcttccttt tccccacccc aacccccaag
    11761 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaagccctg ccatagccac
    11821 tacgggttaa ttaaaaacct aatgatgcct gatgtttcaa tcaggagatc cgaaccagat
    11881 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
    11941 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
    12001 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
    12061 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
//
