LOCUS       sequence-334230-       10779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of envA-expressing stable cell lines, resistant to the 
            antibiotic Neomycin (Gentamicin/G418).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: pLV-EF1a-N2c_envA-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..10779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5331..5880
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5475..5492)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5702..5721
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5883..6677
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5937..5956)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6547..6566
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6701..7289
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6754..6774)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7172..7183)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7497..8130
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8259..8275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8259..8275)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8272..8294)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8283..8299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8307..8313,8314..8331,8332..8337))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8352..8373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8490..8507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8661..9246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8741..8760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9417..10208,10209..10277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10040..10059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10278..10382)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10430..10564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10467..10486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10521..10540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcgg ccgcatggaa gccgtcataa aggcatttct
     3421 gactgggcac cctggaaagg tgagcaagaa ggactccaag aagaagccgc cagcaacaag
     3481 caagaaagac ccggagaaga cgcccttgct gccaacgaga gttaattata ttattggtgt
     3541 cctggtcttg tgtgaggtaa cgggggtaag agctgatgtc cacttactcg agcagccagg
     3601 gaacctttgg attacatggg ccaaccgtac aggccaaacg gatttttgcc tctctacaca
     3661 gtcagccacc tccccttttc aaacatgttt gataggtatc ccgtccccta tttccgaggg
     3721 tgattttaag ggatatgttt ctgatacaaa ttgcaccacc ttgggaactg atcggttagt
     3781 ctcgtcagcc gactttactg gcggacctga caacagtacc accctcactt atcggaaggt
     3841 ctcatgcttg ttgttaaagc tgaatgtctc tatgtgggat gagccacctg aactacagct
     3901 gttaggttcc cagtctctcc ctaacattac taatattgct cagatttccg gtataaccgg
     3961 gggatgcgta ggcttcagac cacaaggggt tccttggtat ctaggttggt ctagacagga
     4021 ggccacgcgg tttctcctta gacacccctc tttctctaaa tccacggaac cgtttacggt
     4081 ggtgacagcg gataggcaca atctttttat ggggagtgag tactgcggtg catatggcta
     4141 cagattttgg aacatgtata actgctcaca ggtggggcgg cagtaccgct gtggtaatgc
     4201 gcgcagcccc cgcccgggtc ttcctgaaat ccagtgtaca aggagaggag gcaaatgggt
     4261 taatcaatca caggaaatta atgagtcgga gccgttcagc tttacggtga actgtacagc
     4321 tagtagtttg ggtaatgcca gtgggtgttg cggaaaagca ggcacgattc tcccgggaaa
     4381 gtgggtcgac agcacacaag gtagtttcac caaaccaaaa gcgctaccac ccgcaatttt
     4441 cctcatttgt ggggatcgcg catggcaagg aattcccagt cgtccggtag ggggcccctg
     4501 ctatttaggc aagcttacca tgttagcacc taagcataca gatattctca aggtgcttgt
     4561 caattcatcg cggacaggta taagacgtaa acgaagcacc tcacacctgg atgatacatg
     4621 ctcagatgaa gtgcagcttt ggggtcctac agcaagaatc tttgcatcta tcctagcccc
     4681 gggggtagca gctgcgcaag ccttaagaga aattgagaga ctagcctgtt ggtccgttaa
     4741 acaggctaac ttgacaacat cactcctcgg ggacttattg gatgatgtca cgagtattcg
     4801 acacgcggtc ctgcagaacc gagcggctat tgacttcttg ctcctagctc acggccatgg
     4861 ctgtgaggac gttgccggaa tgtgctgttt caatttgagt gatcacagtg agtctataca
     4921 gaagaagttc cagctaatga aggaacatgt caataagatc ggcgtggaca gcgacccaat
     4981 tggaagttgg ctgcgaggac tattcggggg aataggagaa tgggccgttc atttgctgaa
     5041 aggactgctt ttggggcttg tagttatttt gttgctagta gtgtgcctgc cttgcagaag
     5101 agccaatcga ccagaatcga aacaacgcag ttttggaggg acagggggga atgtgtcagt
     5161 cacttcccaa agcggaaaag tcataccttc atgggaatca tataagagtg gaggtgagat
     5221 cagactgtaa gcggccgctc tagagtcgac gggccgcggt aacaattgtt aactaactta
     5281 agctagcaac ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg
     5341 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     5401 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     5461 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     5521 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     5581 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     5641 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     5701 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     5761 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     5821 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
     5881 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5941 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6001 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6061 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6121 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6181 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6241 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6301 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6361 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6421 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6481 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6541 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6601 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6661 ttgacgagtt cttctgaccc gggcggggcg cgtctggaac aatcaacctc tggattacaa
     6721 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6781 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6841 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6901 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6961 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7021 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7081 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     7141 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7201 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7261 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     7321 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     7381 gaagcacaag aggaggagga ggtgggtttt tccagtcaca cctcaggtac ctttaagacc
     7441 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     7501 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7561 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     7621 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     7681 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7741 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7801 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7861 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7921 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8101 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8161 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
     8221 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8281 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8341 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8401 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8461 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8521 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8581 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8641 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8701 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8821 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8881 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9001 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9061 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9121 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9181 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9241 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9301 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9361 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9421 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9481 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9541 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9601 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9661 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9721 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9781 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9841 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9901 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9961 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10021 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10081 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10141 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10201 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10261 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10321 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10381 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
    10441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10561 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10621 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10681 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag
    10741 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
