LOCUS       sequence-334253-       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 soluble spike trimer protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R620-M11-303: CMV51p> SARS-CoV-2
            S(1-1213)-2P-...
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(540..559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    709..729
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        740..1043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1044..1247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1198..1218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1244..1268
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1313..1337
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4979..4996
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5090..5107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(5109..5133)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5195..5213)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5196..5213)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5233..5250)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5239..5463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5509..5937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5596..5615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5806..5827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5946..5966)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5951..6280
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6131..6266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6193..6212
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6347..7141
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6401..6420)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7011..7030
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7315..7436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7352..7371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7406..7425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7485..7501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7485..7501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7498..7520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7509..7525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7533..7539,7540..7557,7558..7563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7578..7599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7716..7733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7887..8475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7967..7986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8646..9437,9438..9506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9269..9288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9507..9611)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(9846..11132,1..503)
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
       61 atcctaatag agattagggt agtatatgct atcctaattt atatctgggt agcatatact
      121 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc
      181 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
      241 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
      301 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
      361 taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc
      421 agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat
      481 tttcgctgct tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt
      541 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
      601 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
      661 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata
      721 gaaaagttgg ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      781 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      841 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      901 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      961 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
     1021 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
     1081 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     1141 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
     1201 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     1261 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt
     1321 gtacaaaaaa gttggcacca tgttcgtgtt ccttgtgctg ctgccactcg tgtcatcgca
     1381 gtgcgtgaac ctgaccactc gaacacagct gccaccggcc tacaccaact cattcacccg
     1441 gggagtgtac taccctgaca aagtgttcag gagctcagtg ctgcacagca cccaggacct
     1501 gttcctcccg ttcttctcga acgtcacctg gtttcacgcg atccacgtgt ccggaactaa
     1561 cggaaccaag cgcttcgaca accccgtgct gccttttaac gacggcgtct acttcgcgtc
     1621 caccgagaag tccaacatta tcagaggctg gatcttcgga actaccctgg actccaagac
     1681 ccaaagcctg ctcattgtga acaatgccac caacgttgtg atcaaagtct gcgaattcca
     1741 gttttgcaac gacccgtttc ttggcgtgta ctaccacaag aacaacaaga gctggatgga
     1801 gtccgagttc agagtctatt cgtccgccaa caactgcacc ttcgaatacg tgtcacagcc
     1861 tttcctgatg gacctggagg gaaagcaggg gaacttcaag aacctccgcg aattcgtgtt
     1921 taagaacatc gatggttact ttaagatcta ctccaaacat actcccatta atctcgtgcg
     1981 ggacctaccg cagggattca gcgccctgga gccactggtg gacctcccca tcggcatcaa
     2041 catcacccgc tttcaaaccc tgttggcgct gcatcggagc tacctgaccc ctggagattc
     2101 ctcctctggt tggactgccg gagcagctgc atactacgtg ggctatctcc agccgcggac
     2161 cttcctgctt aagtacaatg agaacggtac tatcaccgac gccgtggatt gtgctctgga
     2221 ccctctgtcc gaaaccaagt gcaccctgaa gtcctttact gtggaaaagg gaatctacca
     2281 gacctccaat ttccgggtgc agcctaccga gtccattgtc cggttcccca acatcactaa
     2341 cctgtgtcct ttcggggaag tctttaatgc cacccgcttc gcctccgtgt acgcatggaa
     2401 ccgcaagcgc atcagcaact gcgtcgccga ctacagcgtg ctgtacaact ccgcgtcctt
     2461 ctcaaccttc aagtgctacg gggtgtcccc gactaagctg aacgacctgt gcttcacgaa
     2521 cgtgtacgcc gactccttcg tgatccgggg ggacgaagtc agacagattg cacccggaca
     2581 gactggaaag atcgcagact acaattacaa gctcccggat gacttcactg gttgcgtgat
     2641 cgcttggaac tccaacaacc tcgacagcaa agtcggcggc aactacaact atctgtaccg
     2701 gctgttccgg aagtccaacc tcaagccatt cgagcgggac atctcgaccg agatctacca
     2761 ggccggttcg actccttgca acggcgtgga ggggttcaac tgctatttcc cgctgcaaag
     2821 ctacggattc cagcccacca acggagtggg ctaccaaccg taccgggtgg tcgtcctgtc
     2881 cttcgaactg ctgcacgccc ctgctaccgt ctgcggtcca aagaagtcca ccaaccttgt
     2941 gaagaacaaa tgcgtgaact tcaacttcaa cggactcact ggcactggag tgctgactga
     3001 gagcaacaag aagttcctgc cgttccaaca atttggcaga gacatcgccg acaccactga
     3061 tgccgtccgc gatcctcaga ctctcgagat tttggatatt accccctgct cctttggggg
     3121 agtcagcgtg atcaccccgg ggaccaacac cagtaaccag gtcgcggtgc tgtaccagga
     3181 cgtgaactgt accgaagtcc cggtggccat ccacgctgac caattgaccc caacttggag
     3241 ggtctactca acggggtcca acgtgttcca aacccgcgca ggatgcctga ttggagccga
     3301 gcacgtgaac aactcctacg agtgcgacat ccctatcggt gccggaattt gtgcgagcta
     3361 ccagacacag acgaactcgc ctgcttccgt ggcctcgcag tccattattg cctacactat
     3421 gagcctcggt gccgaaaact ccgtggccta ctcgaacaac agcatcgcca ttcccaccaa
     3481 cttcacgatc tcggtgacca ccgaaatact gccggtgtcc atgactaaga cctcagtgga
     3541 ttgcacaatg tacatctgtg gcgactccac cgagtgctcc aatctcctcc tgcaatacgg
     3601 ctccttctgt acccaactga accgggcctt aactggcatc gcggtggagc aggacaagaa
     3661 tacccaggaa gtgttcgccc aagtcaaaca gatctacaag actccgccca tcaaggactt
     3721 cggcggcttc aatttctcgc aaatcctccc ggacccttca aagccctcca agcggtcctt
     3781 tattgaggac cttttgttca acaaggtcac cttggccgat gccggcttca tcaagcagta
     3841 cggagactgc ctgggagata ttgccgcgcg ggatctgatc tgcgcccaga agttcaacgg
     3901 tctgactgtg ctgccccccc tcttgaccga cgaaatgatt gctcagtaca cctccgcgct
     3961 gcttgctggc accattacca gcgggtggac gttcggagca ggagccgcac tccaaatccc
     4021 gttcgccatg caaatggcgt atcggttcaa tggtatcggc gtgacccaga acgtgctgta
     4081 cgagaaccag aagctgatcg ctaaccagtt caactccgcc atcggaaaga ttcaggactc
     4141 actgtcatct actgcgtccg ctctgggaaa gctccaggac gtggtcaatc agaacgccca
     4201 ggccctcaac acgctcgtga agcagttgag ctccaacttc ggcgccatct cgagcgtgct
     4261 gaacgatatc ctgagcagac tggatcctcc cgaggccgaa gtgcaaatcg accggttgat
     4321 caccggcaga ctgcagtcgc tgcagaccta cgtaacccag cagttgatca gggctgccga
     4381 gatcagggcc tcggccaatc tggcggccac caagatgtcg gaatgcgtgc tggggcagtc
     4441 taagcgcgtg gacttctgcg gaaagggtta ccacctgatg tcctttccgc aatcggcccc
     4501 tcacggagtg gtgtttctgc atgtcaccta cgtgcccgcc caagaaaaga acttcaccac
     4561 tgcaccggcc atctgccacg acgggaaggc gcatttcccg agagaagggg tgttcgtgag
     4621 caacgggacg cactggttcg tgacccagcg gaacttctac gagcctcaga tcatcactac
     4681 cgacaacacc ttcgtgagcg ggaactgtga tgtcgtgatt ggaattgtca acaacactgt
     4741 gtacgaccca ctgcagcccg aactggatag ctttaaggag gaactggaca agtacttcaa
     4801 aaaccatacc tcacccgatg tggacctggg cgacatctcc ggaatcaacg cctccgtggt
     4861 caacatccaa aaggagattg accgcctgaa cgaagtcgcc aagaacctga acgaatcgct
     4921 gattgatctc caagaactgg gcaaatacga gcagtacatc aagtggccgt cgggcagact
     4981 tgtgccgaga ggttcgccgg gaagcggata cattcctgaa gccccaaggg acggacaggc
     5041 gtacgtgcgc aaggacggag aatgggtgct gctgtcgact ttcctgggac accatcacca
     5101 ccatcactaa tcaactttct tgtacaaagt tgtgaaattc tgcagatatc catcacactg
     5161 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
     5221 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5281 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5341 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5401 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5461 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5521 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5581 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5641 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5701 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5761 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5821 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5881 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     5941 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6001 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc taggctcccc
     6061 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6121 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6181 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6241 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     6301 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     6361 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6421 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6481 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6541 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6601 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6661 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6721 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6781 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6841 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6901 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6961 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7021 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7081 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7141 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7201 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7261 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7441 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7561 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7621 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7681 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7741 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7801 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7861 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7981 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8041 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8101 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8161 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8221 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8281 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8341 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8401 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8461 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8521 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8581 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8641 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8701 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8761 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8821 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8881 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8941 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9001 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9061 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9121 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9181 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9241 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9301 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9361 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9421 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9481 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9541 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9601 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatccg
     9661 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     9721 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     9781 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     9841 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     9901 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     9961 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
    10021 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
    10081 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
    10141 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
    10201 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
    10261 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
    10321 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
    10381 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
    10441 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
    10501 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
    10561 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
    10621 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
    10681 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
    10741 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
    10801 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
    10861 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
    10921 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
    10981 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
    11041 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
    11101 ctatcctaat ctatatctgg gtagcatatg ct
//
