LOCUS       sequence-334257-       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 soluble spike trimer protein 
            (B.1.1.7 variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R620-M13-303: CMV51p> SARS-CoV-2
            S(1-1213)-2P-...
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(421..440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    590..610
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        621..924
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        925..1128
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1079..1099
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1125..1149
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1194..1218
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4860..4877
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             4971..4988
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(4990..5014)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5076..5094)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5077..5094)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5114..5131)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5120..5344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5390..5818
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5477..5496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5687..5708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5827..5847)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5832..6161
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6012..6147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6074..6093
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6228..7022
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6282..6301)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6892..6911
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7196..7317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7233..7252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7287..7306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7366..7382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7366..7382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7379..7401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7390..7406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7414..7420,7421..7438,7439..7444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7459..7480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7597..7614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7768..8356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7848..7867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8527..9318,9319..9387))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9150..9169
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9388..9492)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(9727..11132,1..384)
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
       61 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
      121 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
      181 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
      241 ctaatctgta tccgggtagc atatgctatc ctcatgcata tacagtcagc atatgatacc
      301 cagtagtaga gtgggagtgc tatcctttgc atatgccgcc acctcccaag ggggcgtgaa
      361 ttttcgctgc ttgtcctttt cctgctgggg atctcccgat cccctatggt gcactctcag
      421 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
      481 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      541 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgatcac aactttgtat
      601 agaaaagttg gccctgcagg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      661 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      721 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      781 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
      841 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
      901 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
      961 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     1021 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     1081 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     1141 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaacaactt
     1201 tgtacaaaaa agttggcacc atgttcgtgt tccttgtcct cctgccgctg gtgtcatcgc
     1261 agtgcgtgaa cctgaccacc cggactcagc tgcctcctgc ttacactaac tccttcactc
     1321 gcggggtgta ctaccctgac aaagtgttcc ggtcgtccgt gctgcatagc acccaggacc
     1381 tgttccttcc gttcttctcc aacgtgacct ggttccacgc catttcgggg accaacggca
     1441 ctaagagatt cgacaacccc gtcttgccct tcaatgacgg agtatacttc gcctcgacgg
     1501 aaaagtccaa catcattcgg ggttggatct tcggcactac cctggacagc aagacccagt
     1561 ccctgctcat tgtcaacaac gccaccaacg tcgtgatcaa agtctgcgag tttcagtttt
     1621 gtaacgatcc gttcctcggc gtgtaccaca agaacaacaa gagctggatg gagtccgaat
     1681 tccgggtcta ctcctccgcc aacaactgta ccttcgaata cgtgtcccaa cctttcctca
     1741 tggacctcga aggaaaacag ggaaacttca agaacttgag agagtttgtg ttcaagaata
     1801 tcgatggcta cttcaagatc tactcgaagc ataccccgat caacctcgtg cgggacctac
     1861 ctcaagggtt ctccgcactt gagccgctcg tggacctccc cattggcatc aacatcacca
     1921 gattccagac tttactggca ctgcatagat cgtatctcac acccggggac tcctcctccg
     1981 ggtggacagc tggtgctgcg gcctactacg tgggatatct ccagccccgg accttcttgc
     2041 tgaagtacaa cgagaacggc accatcacgg atgccgtgga ctgtgcactg gaccccttga
     2101 gtgaaaccaa gtgcacgctg aagtccttca ccgtggaaaa aggcatctac cagaccagca
     2161 acttcagggt gcagcctact gaatcaatcg tgcggttccc taacattacc aatctgtgcc
     2221 cgttcggcga agtgttcaac gccactagat tcgcctcggt gtacgcctgg aaccgaaagc
     2281 ggatcagcaa ctgcgtggcc gactactcag tgctctacaa ctcggccagc ttctccacct
     2341 tcaagtgcta cggagtgtcg cccaccaagc tgaacgatct gtgcttcacc aacgtgtacg
     2401 cggattcctt cgtgatccgc ggagatgaag tccgccaaat cgcccccggt caaaccggaa
     2461 agattgccga ctacaactat aagctgccgg acgactttac cggatgcgtg attgcctgga
     2521 attccaacaa tttggattca aaagtcggcg gcaactacaa ctacctgtac cgcctgttcc
     2581 ggaagtcaaa cctgaagccg ttcgaacgcg acatcagcac cgaaatctat caagcgggaa
     2641 gcactccctg caacggggtg gagggcttca actgctactt cccgctgcaa agctacggat
     2701 tccagcctac ctacggcgtt ggataccagc catatcgcgt ggtcgtgctc tcctttgaac
     2761 ttctgcacgc ccctgccact gtgtgcggcc ccaagaagtc cactaacctc gtcaagaata
     2821 agtgcgtgaa tttcaacttt aacggattga ccgggaccgg agtgctcacc gagtccaaca
     2881 agaaattcct gccgttccaa cagtttggac gggacatcga tgatactacg gatgctgtgc
     2941 gggacccaca gactctggag atcctggaca ttactccctg ttcctttggc ggggtgtcgg
     3001 tcatcacccc tggtactaac accagcaacc aggtcgccgt gctgtatcag ggggtgaact
     3061 gcaccgaagt gcccgtggcg atccacgccg accagctgac tccaacttgg cgcgtgtact
     3121 cgactggttc gaacgtgttc cagactcggg ccggttgcct gatcggagcg gagcatgtca
     3181 acaacagcta cgagtgcgac atccctattg gagccgggat ttgcgccagc taccagaccc
     3241 agaccaattc gcatgggtcg gccagcagcg tggcttccca atcgatcatc gcctacacca
     3301 tgtccctggg agccgagaac tcagtggcgt actccaacaa ctcgattgcc atccccatca
     3361 atttcacgat cagcgtgacc accgagatcc tgcctgtgtc catgaccaag acctccgtgg
     3421 actgcaccat gtacatctgc ggagactcca ccgagtgctc caacctcctg ctgcaatacg
     3481 gctcgttctg tacccaactg aaccgagccc tgactgggat cgccgtggag caggacaaga
     3541 acacacagga ggtgttcgca caagtcaagc agatctacaa gaccccgccc atcaaggatt
     3601 tcggagggtt caatttctcc caaattctgc cggacccttc caagccgtcc aagaggtcat
     3661 tcattgagga tctcctgttc aacaaggtca ccctggccga tgctggtttc atcaagcagt
     3721 acggagactg cctgggggac atcgcagccc gcgatctgat ctgcgcccaa aagttcaacg
     3781 gcctgaccgt cctgcctccg cttctgactg atgaaatgat tgcccagtac acctctgcgc
     3841 tgctggctgg cactatcacc tccggctgga ccttcggcgc tggagcagca ctgcagattc
     3901 catttgccat gcaaatggcc taccgcttca acggaatcgg agtgactcag aacgtcctgt
     3961 acgagaacca gaagctcatc gccaaccagt tcaactccgc cattgggaag attcaggatt
     4021 ccctgtcgag cactgcgtca gccctgggaa agctgcagga cgtcgtgaac cagaacgcac
     4081 aggccctgaa cacccttgtg aagcagctgt cgtcgaactt cggggcgatc tcctccgtgt
     4141 tgaacgacat cctggcccgg ctggacccgc ctgaggcaga agtgcagatc gaccggctga
     4201 ttaccggacg cctccagtcc cttcaaactt atgtgaccca gcagctgatt cgcgcggcgg
     4261 aaattcgggc tagcgccaat ctggctgcca ccaagatgag cgaatgcgtg cttggacagt
     4321 ccaagagagt ggacttttgt ggaaagggct accacctgat gagcttcccc caatccgccc
     4381 cacacggtgt cgtgtttctg cacgtgacct acgtgccggc gcaggaaaag aacttcacca
     4441 ctgcccctgc catctgccac gatgggaagg cgcacttccc gagggaggga gtgttcgtgt
     4501 caaacggaac ccactggttc gtgacccaaa ggaacttcta cgagccccag attatcacca
     4561 cccacaacac tttcgtgagc ggcaactgcg acgtggtcat cggcatcgtg aacaacactg
     4621 tgtacgaccc gctgcaaccg gagctcgata gctttaagga agaactcgac aagtacttta
     4681 agaatcatac gtcccccgat gtggatctgg gcgacattag cggtatcaac gcgtctgtcg
     4741 tgaacattca gaaggagatt gacaggctca acgaagtcgc caaaaacctg aacgagagcc
     4801 tgatagactt gcaggaactg ggaaaatacg agcagtacat caagtggccc agcggacgcc
     4861 ttgtcccgag gggaagccct ggctccggtt acatccccga agctccacgg gacggacagg
     4921 cctacgtgcg caaggacgga gaatgggtgc tgctgagcac cttcctcggg catcatcacc
     4981 accaccacta atcaactttc ttgtacaaag ttgtgaaatt ctgcagatat ccatcacact
     5041 ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga
     5101 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5161 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5221 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5281 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5341 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5761 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5821 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5881 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ctaggctccc
     5941 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6001 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6061 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6121 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     6181 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     6241 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6301 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6361 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6421 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6481 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6541 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6601 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6661 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6721 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6781 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6841 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6901 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6961 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     7021 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     7081 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7141 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7321 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7381 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7441 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7501 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7561 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7621 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7681 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7741 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7801 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7861 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7921 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7981 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8041 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8101 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8161 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8221 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8281 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8341 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8401 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8461 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8521 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8581 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8641 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8701 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8761 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8821 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8881 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8941 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9001 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9061 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9121 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9181 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9241 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9301 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9361 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9421 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9481 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcc
     9541 gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc
     9601 caggggccca ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc
     9661 taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta
     9721 accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta
     9781 attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag
     9841 ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg
     9901 gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa
     9961 ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct
    10021 aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt
    10081 ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat
    10141 gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca
    10201 ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg
    10261 tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg
    10321 gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct
    10381 atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct
    10441 aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg
    10501 gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct
    10561 gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca
    10621 ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa
    10681 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac
    10741 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga
    10801 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat
    10861 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    10921 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
    10981 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    11041 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat
    11101 gctatcctaa tttatatctg ggtagcatat ac
//
