LOCUS       sequence-334260-       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 soluble spike trimer protein 
            (B.1.351 variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R620-M14-303: CMV51p> SARS-CoV-2
            S(1-1213)-2P-...
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(406..425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    575..595
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        606..909
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        910..1113
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1064..1084
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1110..1134
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4845..4862
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             4956..4973
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(4975..4999)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5061..5079)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5062..5079)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5099..5116)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5105..5329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5375..5803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5462..5481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5672..5693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5812..5832)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5817..6146
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5997..6132
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6059..6078
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6213..7007
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6267..6286)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6877..6896
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7181..7302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7218..7237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7272..7291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7351..7367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7351..7367)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7364..7386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7375..7391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7399..7405,7406..7423,7424..7429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7444..7465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7582..7599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7753..8341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7833..7852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8512..9303,9304..9372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9135..9154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9373..9477)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(9712..11132,1..369)
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
       61 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
      121 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
      181 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
      241 gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg
      301 agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc
      361 cttttcctgc tggggatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      421 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      481 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      541 gcttagggtt aggcgttttg cgctgcttcg atcacaactt tgtatagaaa agttggccct
      601 gcaggcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      661 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      721 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      781 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      841 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      901 attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca
      961 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1021 caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg
     1081 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1141 agacgccatc cacgctgttt tgacctccat agaagacaac aactttgtac aaaaaagttg
     1201 gcaccatgtt cgtgttcctt gtcctcctgc cgctggtgtc atcgcagtgc gtgaacttca
     1261 ccacccggac tcagctgcct cctgcttaca ctaactcctt cactcgcggg gtgtactacc
     1321 ctgacaaagt gttccggtcg tccgtgctgc atagcaccca ggacctgttc cttccgttct
     1381 tctccaacgt gacctggttc cacgccattc acgtgtcggg gaccaacggc actaagagat
     1441 tcgccaaccc cgtcttgccc ttcaatgacg gagtatactt cgcctcgacg gaaaagtcca
     1501 acatcattcg gggttggatc ttcggcacta ccctggacag caagacccag tccctgctca
     1561 ttgtcaacaa cgccaccaac gtcgtgatca aagtctgcga gtttcagttt tgtaacgatc
     1621 cgttcctcgg cgtgtactac cacaagaaca acaagagctg gatggagtcc gaattccggg
     1681 tctactcctc cgccaacaac tgtaccttcg aatacgtgtc ccaacctttc ctcatggacc
     1741 tcgaaggaaa acagggaaac ttcaagaact tgagagagtt tgtgttcaag aatatcgatg
     1801 gctacttcaa gatctactcg aagcataccc cgatcaacct cgtgcgggga ctacctcaag
     1861 ggttctccgc acttgagccg ctcgtggacc tccccattgg catcaacatc accagattcc
     1921 agactctgca tatctcgtat ctcacacccg gggactcctc ctccgggtgg acagctggtg
     1981 ctgcggccta ctacgtggga tatctccagc cccggacctt cttgctgaag tacaacgaga
     2041 acggcaccat cacggatgcc gtggactgtg cactggaccc cttgagtgaa accaagtgca
     2101 cgctgaagtc cttcaccgtg gaaaaaggca tctaccagac cagcaacttc agggtgcagc
     2161 ctactgaatc aatcgtgcgg ttccctaaca ttaccaatct gtgcccgttc ggcgaagtgt
     2221 tcaacgccac tagattcgcc tcggtgtacg cctggaaccg aaagcggatc agcaactgcg
     2281 tggccgacta ctcagtgctc tacaactcgg ccagcttctc caccttcaag tgctacggag
     2341 tgtcgcccac caagctgaac gatctgtgct tcaccaacgt gtacgcggat tccttcgtga
     2401 tccgcggaga tgaagtccgc caaatcgccc ccggtcaaac cggaaacatt gccgactaca
     2461 actataagct gccggacgac tttaccggat gcgtgattgc ctggaattcc aacaatttgg
     2521 attcaaaagt cggcggcaac tacaactacc tgtaccgcct gttccggaag tcaaacctga
     2581 agccgttcga acgcgacatc agcaccgaaa tctatcaagc gggaagcact ccctgcaacg
     2641 gggtgaaggg cttcaactgc tacttcccgc tgcaaagcta cggattccag cctacctacg
     2701 gcgttggata ccagccatat cgcgtggtcg tgctctcctt tgaacttctg cacgcccctg
     2761 ccactgtgtg cggccccaag aagtccacta acctcgtcaa gaataagtgc gtgaatttca
     2821 actttaacgg attgaccggg accggagtgc tcaccgagtc caacaagaaa ttcctgccgt
     2881 tccaacagtt tggacgggac atcgccgata ctacggatgc tgtgcgggac ccacagactc
     2941 tggagatcct ggacattact ccctgttcct ttggcggggt gtcggtcatc acccctggta
     3001 ctaacaccag caaccaggtc gccgtgctgt atcagggggt gaactgcacc gaagtgcccg
     3061 tggcgatcca cgccgaccag ctgactccaa cttggcgcgt gtactcgact ggttcgaacg
     3121 tgttccagac tcgggccggt tgcctgatcg gagcggagca tgtcaacaac agctacgagt
     3181 gcgacatccc tattggagcc gggatttgcg ccagctacca gacccagacc aattcgcctg
     3241 ggtcggccag cagcgtggct tcccaatcga tcatcgccta caccatgtcc ctgggagtgg
     3301 agaactcagt ggcgtactcc aacaactcga ttgccatccc cactaatttc acgatcagcg
     3361 tgaccaccga gatcctgcct gtgtccatga ccaagacctc cgtggactgc accatgtaca
     3421 tctgcggaga ctccaccgag tgctccaacc tcctgctgca atacggctcg ttctgtaccc
     3481 aactgaaccg agccctgact gggatcgccg tggagcagga caagaacaca caggaggtgt
     3541 tcgcacaagt caagcagatc tacaagaccc cgcccatcaa ggatttcgga gggttcaatt
     3601 tctcccaaat tctgccggac ccttccaagc cgtccaagag gtcattcatt gaggatctcc
     3661 tgttcaacaa ggtcaccctg gccgatgctg gtttcatcaa gcagtacgga gactgcctgg
     3721 gggacatcgc agcccgcgat ctgatctgcg cccaaaagtt caacggcctg accgtcctgc
     3781 ctccgcttct gactgatgaa atgattgccc agtacacctc tgcgctgctg gctggcacta
     3841 tcacctccgg ctggaccttc ggcgctggag cagcactgca gattccattt gccatgcaaa
     3901 tggcctaccg cttcaacgga atcggagtga ctcagaacgt cctgtacgag aaccagaagc
     3961 tcatcgccaa ccagttcaac tccgccattg ggaagattca ggattccctg tcgagcactg
     4021 cgtcagccct gggaaagctg caggacgtcg tgaaccagaa cgcacaggcc ctgaacaccc
     4081 ttgtgaagca gctgtcgtcg aacttcgggg cgatctcctc cgtgttgaac gacatcctgt
     4141 cccggctgga cccgcctgag gcagaagtgc agatcgaccg gctgattacc ggacgcctcc
     4201 agtcccttca aacttatgtg acccagcagc tgattcgcgc ggcggaaatt cgggctagcg
     4261 ccaatctggc tgccaccaag atgagcgaat gcgtgcttgg acagtccaag agagtggact
     4321 tttgtggaaa gggctaccac ctgatgagct tcccccaatc cgccccacac ggtgtcgtgt
     4381 ttctgcacgt gacctacgtg ccggcgcagg aaaagaactt caccactgcc cctgccatct
     4441 gccacgatgg gaaggcgcac ttcccgaggg agggagtgtt cgtgtcaaac ggaacccact
     4501 ggttcgtgac ccaaaggaac ttctacgagc cccagattat caccaccgat aacactttcg
     4561 tgagcggcaa ctgcgacgtg gtcatcggca tcgtgaacaa cactgtgtac gacccgctgc
     4621 aaccggagct cgatagcttt aaggaagaac tcgacaagta ctttaagaat catacgtccc
     4681 ccgatgtgga tctgggcgac attagcggta tcaacgcgtc tgtcgtgaac attcagaagg
     4741 agattgacag gctcaacgaa gtcgccaaaa acctgaacga gagcctgata gacttgcagg
     4801 aactgggaaa atacgagcag tacatcaagt ggccatccgg tcgcctcgtg ccaagaggat
     4861 ccccaggctc cggttacatc cccgaagctc cacgggacgg acaggcctac gtgcgcaagg
     4921 acggagaatg ggtgctgctg agcaccttcc tcgggcatca tcaccaccac cactaatcaa
     4981 ctttcttgta caaagttgtg aaattctgca gatatccatc acactggcgg ccgctcgagc
     5041 atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     5101 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5161 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5221 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5281 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5341 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5581 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5701 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     5821 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5881 atctcaatta gtcagcaacc aggtgtggaa agtccctagg ctccccagca ggcagaagta
     5941 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6001 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6061 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     6121 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     6181 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6241 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6301 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6361 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6421 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6481 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6541 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6601 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6661 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6721 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6781 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6841 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6901 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6961 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     7021 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     7081 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7141 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7261 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7321 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7381 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7441 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7501 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7561 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8641 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8821 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9361 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9421 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9481 atttccccga aaagtgccac ctgacgtcga cggatcggga gatccgcctt tatgtgtaac
     9541 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     9601 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     9661 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     9721 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     9781 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     9841 ggatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     9901 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     9961 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
    10021 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
    10081 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
    10141 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
    10201 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
    10261 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
    10321 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
    10381 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
    10441 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
    10501 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
    10561 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
    10621 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
    10681 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
    10741 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
    10801 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
    10861 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
    10921 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
    10981 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
    11041 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
    11101 atctgggtag catatactac ccaaatatct gg
//
