LOCUS       sequence-334262-       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 soluble spike trimer protein 
            (B.1.429 variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R620-M21-303: CMV51p> SARS-CoV-2
            S(1-1213)-2P-...
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(365..384)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    534..554
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        565..868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        869..1072
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1023..1043
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1069..1093
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1138..1162
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4804..4821
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             4915..4932
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(4934..4958)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5020..5038)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5021..5038)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5058..5075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5064..5288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5334..5762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5421..5440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5631..5652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5771..5791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5776..6105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5956..6091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6018..6037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6172..6966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6226..6245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6836..6855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7140..7261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7177..7196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7231..7250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7310..7326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7310..7326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7323..7345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7334..7350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7358..7364,7365..7382,7383..7388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7403..7424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7541..7558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7712..8300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7792..7811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8471..9262,9263..9331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9094..9113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9332..9436)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(9671..11132,1..328)
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
       61 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
      121 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
      181 tatcctaatc tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga
      241 tacccagtag tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg
      301 tgaattttcg ctgcttgtcc ttttcctgct ggggatctcc cgatccccta tggtgcactc
      361 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
      421 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
      481 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcga tcacaacttt
      541 gtatagaaaa gttggccctg caggcgttac ataacttacg gtaaatggcc cgcctggctg
      601 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      661 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      721 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg
      781 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat
      841 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg
      901 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      961 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
     1021 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     1081 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaaca
     1141 actttgtaca aaaaagttgg caccatgttc gtgttccttg tgctgctgcc actcgtgtca
     1201 attcagtgcg tgaacctgac cactcgaaca cagctgccac cggcctacac caactcattc
     1261 acccggggag tgtactaccc tgacaaagtg ttcaggagct cagtgctgca cagcacccag
     1321 gacctgttcc tcccgttctt ctcgaacgtc acctggtttc acgcgatcca cgtgtccgga
     1381 actaacggaa ccaagcgctt cgacaacccc gtgctgcctt ttaacgacgg cgtctacttc
     1441 gcgtccaccg agaagtccaa cattatcaga ggctggatct tcggaactac cctggactcc
     1501 aagacccaaa gcctgctcat tgtgaacaat gccaccaacg ttgtgatcaa agtctgcgaa
     1561 ttccagtttt gcaacgaccc gtttcttggc gtgtactacc acaagaacaa caagagctgt
     1621 atggagtccg agttcagagt ctattcgtcc gccaacaact gcaccttcga atacgtgtca
     1681 cagcctttcc tgatggacct ggagggaaag caggggaact tcaagaacct ccgcgaattc
     1741 gtgtttaaga acatcgatgg ttactttaag atctactcca aacatactcc cattaatctc
     1801 gtgcgggacc taccgcaggg attcagcgcc ctggagccac tggtggacct ccccatcggc
     1861 atcaacatca cccgctttca aaccctgttg gcgctgcatc ggagctacct gacccctgga
     1921 gattcctcct ctggttggac tgccggagca gctgcatact acgtgggcta tctccagccg
     1981 cggaccttcc tgcttaagta caatgagaac ggtactatca ccgacgccgt ggattgtgct
     2041 ctggaccctc tgtccgaaac caagtgcacc ctgaagtcct ttactgtgga aaagggaatc
     2101 taccagacct ccaatttccg ggtgcagcct accgagtcca ttgtccggtt ccccaacatc
     2161 actaacctgt gtcctttcgg ggaagtcttt aatgccaccc gcttcgcctc cgtgtacgca
     2221 tggaaccgca agcgcatcag caactgcgtc gccgactaca gcgtgctgta caactccgcg
     2281 tccttctcaa ccttcaagtg ctacggggtg tccccgacta agctgaacga cctgtgcttc
     2341 acgaacgtgt acgccgactc cttcgtgatc cggggggacg aagtcagaca gattgcaccc
     2401 ggacagactg gaaagatcgc agactacaat tacaagctcc cggatgactt cactggttgc
     2461 gtgatcgctt ggaactccaa caacctcgac agcaaagtcg gcggcaacta caactatcgg
     2521 taccggctgt tccggaagtc caacctcaag ccattcgagc gggacatctc gaccgagatc
     2581 taccaggccg gttcgactcc ttgcaacggc gtggaggggt tcaactgcta tttcccgctg
     2641 caaagctacg gattccagcc caccaacgga gtgggctacc aaccgtaccg ggtggtcgtc
     2701 ctgtccttcg aactgctgca cgcccctgct accgtctgcg gtccaaagaa gtccaccaac
     2761 cttgtgaaga acaaatgcgt gaacttcaac ttcaacggac tcactggcac tggagtgctg
     2821 actgagagca acaagaagtt cctgccgttc caacaatttg gcagagacat cgccgacacc
     2881 actgatgccg tccgcgatcc tcagactctc gagattttgg atattacccc ctgctccttt
     2941 gggggagtca gcgtgatcac cccggggacc aacaccagta accaggtcgc ggtgctgtac
     3001 cagggcgtga actgtaccga agtcccggtg gccatccacg ctgaccaatt gaccccaact
     3061 tggagggtct actcaacggg gtccaacgtg ttccaaaccc gcgcaggatg cctgattgga
     3121 gccgagcacg tgaacaactc ctacgagtgc gacatcccta tcggtgccgg aatttgtgcg
     3181 agctaccaga cacagacgaa ctcgcctgct tccgtggcct cgcagtccat tattgcctac
     3241 actatgagcc tcggtgccga aaactccgtg gcctactcga acaacagcat cgccattccc
     3301 accaacttca cgatctcggt gaccaccgaa atactgccgg tgtccatgac taagacctca
     3361 gtggattgca caatgtacat ctgtggcgac tccaccgagt gctccaatct cctcctgcaa
     3421 tacggctcct tctgtaccca actgaaccgg gccttaactg gcatcgcggt ggagcaggac
     3481 aagaataccc aggaagtgtt cgcccaagtc aaacagatct acaagactcc gcccatcaag
     3541 gacttcggcg gcttcaattt ctcgcaaatc ctcccggacc cttcaaagcc ctccaagcgg
     3601 tcctttattg aggacctttt gttcaacaag gtcaccttgg ccgatgccgg cttcatcaag
     3661 cagtacggag actgcctggg agatattgcc gcgcgggatc tgatctgcgc ccagaagttc
     3721 aacggtctga ctgtgctgcc ccccctcttg accgacgaaa tgattgctca gtacacctcc
     3781 gcgctgcttg ctggcaccat taccagcggg tggacgttcg gagcaggagc cgcactccaa
     3841 atcccgttcg ccatgcaaat ggcgtatcgg ttcaatggta tcggcgtgac ccagaacgtg
     3901 ctgtacgaga accagaagct gatcgctaac cagttcaact ccgccatcgg aaagattcag
     3961 gactcactgt catctactgc gtccgctctg ggaaagctcc aggacgtggt caatcagaac
     4021 gcccaggccc tcaacacgct cgtgaagcag ttgagctcca acttcggcgc catctcgagc
     4081 gtgctgaacg atatcctgag cagactggat cctcccgagg ccgaagtgca aatcgaccgg
     4141 ttgatcaccg gcagactgca gtcgctgcag acctacgtaa cccagcagtt gatcagggct
     4201 gccgagatca gggcctcggc caatctggcg gccaccaaga tgtcggaatg cgtgctgggg
     4261 cagtctaagc gcgtggactt ctgcggaaag ggttaccacc tgatgtcctt tccgcaatcg
     4321 gcccctcacg gagtggtgtt tctgcatgtc acctacgtgc ccgcccaaga aaagaacttc
     4381 accactgcac cggccatctg ccacgacggg aaggcgcatt tcccgagaga aggggtgttc
     4441 gtgagcaacg ggacgcactg gttcgtgacc cagcggaact tctacgagcc tcagatcatc
     4501 actaccgaca acaccttcgt gagcgggaac tgtgatgtcg tgattggaat tgtcaacaac
     4561 actgtgtacg acccactgca gcccgaactg gatagcttta aggaggaact ggacaagtac
     4621 ttcaaaaacc atacctcacc cgatgtggac ctgggcgaca tctccggaat caacgcctcc
     4681 gtggtcaaca tccaaaagga gattgaccgc ctgaacgaag tcgccaagaa cctgaacgaa
     4741 tcgctgattg atctccaaga actgggcaaa tacgagcagt acatcaagtg gccgtcgggc
     4801 agacttgtgc cgagaggttc gccgggaagc ggatacattc ctgaagcccc aagggacgga
     4861 caggcgtacg tgcgcaagga cggagaatgg gtgctgctgt cgactttcct gggacaccat
     4921 caccaccatc actaatcaac tttcttgtac aaagttgtga aattctgcag atatccatca
     4981 cactggcggc cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc
     5041 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccctaggc
     5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     6061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6121 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     6181 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6241 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6301 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     6361 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6421 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6481 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6541 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6601 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6661 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6721 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6781 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6841 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6901 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6961 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7021 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7081 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7261 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     9481 atccgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     9541 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     9601 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     9661 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     9721 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     9781 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     9841 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     9901 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     9961 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
    10021 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
    10081 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
    10141 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
    10201 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
    10261 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
    10321 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
    10381 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
    10441 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
    10501 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
    10561 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
    10621 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
    10681 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
    10741 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
    10801 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10861 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
    10921 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    10981 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
    11041 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
    11101 gctatcctaa tctatatctg ggtagcatat gc
//
