LOCUS       sequence-334264-       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 soluble spike trimer protein 
            (D614G mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R620-M15-303: CMV51p> SARS-CoV-2
            S(1-1213)-2P-...
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(399..418)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    568..588
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        599..902
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        903..1106
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1057..1077
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1103..1127
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1172..1196
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4838..4855
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             4949..4966
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(4968..4992)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5054..5072)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5055..5072)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5092..5109)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5098..5322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5368..5796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5455..5474)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5665..5686
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5805..5825)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5810..6139
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5990..6125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6052..6071
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6206..7000
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6260..6279)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6870..6889
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7174..7295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7211..7230)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7265..7284
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7344..7360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7344..7360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7357..7379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7368..7384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7392..7398,7399..7416,7417..7422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7437..7458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7575..7592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7746..8334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7826..7845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8505..9296,9297..9365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9128..9147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9366..9470)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(9705..11132,1..362)
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
       61 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
      121 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
      181 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
      241 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
      301 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
      361 tgctggggat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
      421 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      481 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      541 gttaggcgtt ttgcgctgct tcgatcacaa ctttgtatag aaaagttggc cctgcaggcg
      601 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      661 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      721 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      781 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      841 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      901 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      961 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1021 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
     1081 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     1141 atccacgctg ttttgacctc catagaagac aacaactttg tacaaaaaag ttggcaccat
     1201 gttcgtgttc cttgtgctgc tgccactcgt gtcatcgcag tgcgtgaacc tgaccactcg
     1261 aacacagctg ccaccggcct acaccaactc attcacccgg ggagtgtact accctgacaa
     1321 agtgttcagg agctcagtgc tgcacagcac ccaggacctg ttcctcccgt tcttctcgaa
     1381 cgtcacctgg tttcacgcga tccacgtgtc cggaactaac ggaaccaagc gcttcgacaa
     1441 ccccgtgctg ccttttaacg acggcgtcta cttcgcgtcc accgagaagt ccaacattat
     1501 cagaggctgg atcttcggaa ctaccctgga ctccaagacc caaagcctgc tcattgtgaa
     1561 caatgccacc aacgttgtga tcaaagtctg cgaattccag ttttgcaacg acccgtttct
     1621 tggcgtgtac taccacaaga acaacaagag ctggatggag tccgagttca gagtctattc
     1681 gtccgccaac aactgcacct tcgaatacgt gtcacagcct ttcctgatgg acctggaggg
     1741 aaagcagggg aacttcaaga acctccgcga attcgtgttt aagaacatcg atggttactt
     1801 taagatctac tccaaacata ctcccattaa tctcgtgcgg gacctaccgc agggattcag
     1861 cgccctggag ccactggtgg acctccccat cggcatcaac atcacccgct ttcaaaccct
     1921 gttggcgctg catcggagct acctgacccc tggagattcc tcctctggtt ggactgccgg
     1981 agcagctgca tactacgtgg gctatctcca gccgcggacc ttcctgctta agtacaatga
     2041 gaacggtact atcaccgacg ccgtggattg tgctctggac cctctgtccg aaaccaagtg
     2101 caccctgaag tcctttactg tggaaaaggg aatctaccag acctccaatt tccgggtgca
     2161 gcctaccgag tccattgtcc ggttccccaa catcactaac ctgtgtcctt tcggggaagt
     2221 ctttaatgcc acccgcttcg cctccgtgta cgcatggaac cgcaagcgca tcagcaactg
     2281 cgtcgccgac tacagcgtgc tgtacaactc cgcgtccttc tcaaccttca agtgctacgg
     2341 ggtgtccccg actaagctga acgacctgtg cttcacgaac gtgtacgccg actccttcgt
     2401 gatccggggg gacgaagtca gacagattgc acccggacag actggaaaga tcgcagacta
     2461 caattacaag ctcccggatg acttcactgg ttgcgtgatc gcttggaact ccaacaacct
     2521 cgacagcaaa gtcggcggca actacaacta tctgtaccgg ctgttccgga agtccaacct
     2581 caagccattc gagcgggaca tctcgaccga gatctaccag gccggttcga ctccttgcaa
     2641 cggcgtggag gggttcaact gctatttccc gctgcaaagc tacggattcc agcccaccaa
     2701 cggagtgggc taccaaccgt accgggtggt cgtcctgtcc ttcgaactgc tgcacgcccc
     2761 tgctaccgtc tgcggtccaa agaagtccac caaccttgtg aagaacaaat gcgtgaactt
     2821 caacttcaac ggactcactg gcactggagt gctgactgag agcaacaaga agttcctgcc
     2881 gttccaacaa tttggcagag acatcgccga caccactgat gccgtccgcg atcctcagac
     2941 tctcgagatt ttggatatta ccccctgctc ctttggggga gtcagcgtga tcaccccggg
     3001 gaccaacacc agtaaccagg tcgcggtgct gtaccagggc gtgaactgta ccgaagtccc
     3061 ggtggccatc cacgctgacc aattgacccc aacttggagg gtctactcaa cggggtccaa
     3121 cgtgttccaa acccgcgcag gatgcctgat tggagccgag cacgtgaaca actcctacga
     3181 gtgcgacatc cctatcggtg ccggaatttg tgcgagctac cagacacaga cgaactcgcc
     3241 tgcttccgtg gcctcgcagt ccattattgc ctacactatg agcctcggtg ccgaaaactc
     3301 cgtggcctac tcgaacaaca gcatcgccat tcccaccaac ttcacgatct cggtgaccac
     3361 cgaaatactg ccggtgtcca tgactaagac ctcagtggat tgcacaatgt acatctgtgg
     3421 cgactccacc gagtgctcca atctcctcct gcaatacggc tccttctgta cccaactgaa
     3481 ccgggcctta actggcatcg cggtggagca ggacaagaat acccaggaag tgttcgccca
     3541 agtcaaacag atctacaaga ctccgcccat caaggacttc ggcggcttca atttctcgca
     3601 aatcctcccg gacccttcaa agccctccaa gcggtccttt attgaggacc ttttgttcaa
     3661 caaggtcacc ttggccgatg ccggcttcat caagcagtac ggagactgcc tgggagatat
     3721 tgccgcgcgg gatctgatct gcgcccagaa gttcaacggt ctgactgtgc tgccccccct
     3781 cttgaccgac gaaatgattg ctcagtacac ctccgcgctg cttgctggca ccattaccag
     3841 cgggtggacg ttcggagcag gagccgcact ccaaatcccg ttcgccatgc aaatggcgta
     3901 tcggttcaat ggtatcggcg tgacccagaa cgtgctgtac gagaaccaga agctgatcgc
     3961 taaccagttc aactccgcca tcggaaagat tcaggactca ctgtcatcta ctgcgtccgc
     4021 tctgggaaag ctccaggacg tggtcaatca gaacgcccag gccctcaaca cgctcgtgaa
     4081 gcagttgagc tccaacttcg gcgccatctc gagcgtgctg aacgatatcc tgagcagact
     4141 ggatcctccc gaggccgaag tgcaaatcga ccggttgatc accggcagac tgcagtcgct
     4201 gcagacctac gtaacccagc agttgatcag ggctgccgag atcagggcct cggccaatct
     4261 ggcggccacc aagatgtcgg aatgcgtgct ggggcagtct aagcgcgtgg acttctgcgg
     4321 aaagggttac cacctgatgt cctttccgca atcggcccct cacggagtgg tgtttctgca
     4381 tgtcacctac gtgcccgccc aagaaaagaa cttcaccact gcaccggcca tctgccacga
     4441 cgggaaggcg catttcccga gagaaggggt gttcgtgagc aacgggacgc actggttcgt
     4501 gacccagcgg aacttctacg agcctcagat catcactacc gacaacacct tcgtgagcgg
     4561 gaactgtgat gtcgtgattg gaattgtcaa caacactgtg tacgacccac tgcagcccga
     4621 actggatagc tttaaggagg aactggacaa gtacttcaaa aaccatacct cacccgatgt
     4681 ggacctgggc gacatctccg gaatcaacgc ctccgtggtc aacatccaaa aggagattga
     4741 ccgcctgaac gaagtcgcca agaacctgaa cgaatcgctg attgatctcc aagaactggg
     4801 caaatacgag cagtacatca agtggccgtc gggcagactt gtgccgagag gttcgccggg
     4861 aagcggatac attcctgaag ccccaaggga cggacaggcg tacgtgcgca aggacggaga
     4921 atgggtgctg ctgtcgactt tcctgggaca ccatcaccac catcactaat caactttctt
     4981 gtacaaagtt gtgaaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc
     5041 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     5101 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5161 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5221 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5281 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5341 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5401 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5461 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5521 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5581 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5641 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5701 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5761 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5821 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5881 ttagtcagca accaggtgtg gaaagtccct aggctcccca gcaggcagaa gtatgcaaag
     5941 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6001 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6061 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6121 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6181 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6241 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6301 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6361 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     6421 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6481 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6541 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6601 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6661 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6721 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6781 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6841 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6901 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6961 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     7021 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     7081 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7141 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7201 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7261 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7321 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7381 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7501 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7561 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7621 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7681 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7921 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8161 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8641 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9481 cgaaaagtgc cacctgacgt cgacggatcg ggagatccgc ctttatgtgt aactcttggc
     9541 tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg
     9601 gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag
     9661 agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc
     9721 ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca
     9781 acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc
     9841 tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt
     9901 gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct
     9961 gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt
    10021 gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata
    10081 aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa
    10141 accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag
    10201 aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg
    10261 ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag
    10321 gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga
    10381 gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg
    10441 ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg
    10501 tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa
    10561 gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca
    10621 caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag
    10681 gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca
    10741 cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca
    10801 tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg
    10861 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
    10921 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
    10981 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
    11041 tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg
    11101 tagcatatac tacccaaata tctggatagc at
//
