LOCUS       sequence-334265-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express Dcp2 Delta-H1 WD  at endogenous level in S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Xing W, Muhlrad D, Parker R, Rosen MK
  TITLE     A quantitative inventory of yeast P body proteins reveals principles
            of composition and specificity.
  JOURNAL   Elife. 2020 Jun 19;9. pii: 56525. doi: 10.7554/eLife.56525.
  PUBMED    32553117
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-19"; volume: "9. pii: 56525. doi"; pages: " 
            10.7554/eLife.56525"
COMMENT     Sequence Label: Dcp2 Delta-H1 WD
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3906..4616
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3933..3961)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4556..4576
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      4640..4827
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4695..4719)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4895..4911)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4896..4912)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4937..4956)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4938..4956)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4966..4983)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4966..4982)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4975..4997)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5123..5578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5210..5229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5420..5441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5709..6512)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6513..6728)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6752..6771)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6871..6893
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6931..6949)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6990..7493
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7530..7634
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7635..7703,7704..8495)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7853..7872)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8666..9254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9155..9174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctct cgtcgtaagg ctgacactgc
      481 agaagaaaag tcataatttg aatactagcc ggtatgaaac tgtgattgat taacctgggg
      541 ttacctaaag agaacataag taatactcat gacagaatca aaacacaata caaaatttat
      601 ccgaacctcg gcccgactgc cggctcgccg ggaaagggga caaccgcttc tatccgtcga
      661 ctaacttcat cggcccaatg gaagctatga tatggggatt tccattgagc cgatagcaat
      721 gtagggtaat actgttgcgt atatagtgat agttattgaa ttttattacc ctgcgggaat
      781 attgagacat cactaagcac gaattttacg tctgaggaaa gttgaatgat ggcaaaataa
      841 ccaggaaaaa caaatattga atccttgtga aggattccac agttgtttaa tcctccttaa
      901 gctcacttag tatcaattgt ctaaataata ttgctttgaa tctgaaaaaa ataaaagtac
      961 cttcgcatta gacaatgtca ctgccgctac gacacgcatt ggagaacgtt acttctgttg
     1021 atagaatttt agaggactta ttagtacgtt ttattataaa ttgtccgaat gaagatttat
     1081 cgagtgtcga gagagagtta tttcattttg aagaagcctc agcgttttac acggctttca
     1141 tcaaattgat gaatccaact ttaccctccc taaagattaa atcatttgct caattgatca
     1201 taaaactatg tcctctggtt tggaaatggg acataagagt ggatgaggca ctccagcaat
     1261 tctccaagta taagaaaagt ataccggtga ggggcgctgc catatttaac gagaacctga
     1321 gtaaaatttt attggtacag ggtactgaat cggattcttg gtcattccca agggggaaga
     1381 tatctaaaga tgaaaatgac atagattgtt gcattagaga agtgaaagaa gaaattggtt
     1441 tcgatttgac ggactatatt gacgacaacc aattcattga aagaaatatt caaggtaaaa
     1501 attacaaaat atttttgata tctggtgttt cagaagtctt caattttaaa cctcaagtta
     1561 gaaatgaaat tgataagata gaatggttcg attttaagaa aatttctaaa acaatgtaca
     1621 aatcaaatat caagtattat ctgattaatt ccatgatgag acccttatca atgtggttaa
     1681 ggcatcagag gcaaataaaa aatgaagatc aattgaaatc ctatgcggaa gaacaagcga
     1741 aattgttgtt gggtatcact aaggaggagc agattgatcc cggtagagag ttgctgaata
     1801 tgttacatac tgcagtgcaa gctaacagta ataataatgc ggtctccaac ggacaggtac
     1861 cctcgagcca agagcttcag catttgaaag agcaatcagg agaacacaac caacagaagg
     1921 atcagcagtc atcgttttct tctcaacaac aaccttcaat atttccatct ctttctgaac
     1981 cgtttgctaa caataagaat gttataccac ctactatgcc aatggctaac gtattcatgt
     2041 caaatcctca attgtttgcg acaatgaatg gccagccttt tgcacctttc ccatttatgt
     2101 taccattaac taacaatagt aatagcgcta accctattcc aactccggtc ccccctaatt
     2161 ttaatgctcc tccgaatccg atggcttttg gtgttccaaa catgcataac ctttctggac
     2221 cagcagtatc tcaaccgttt tccttgcctc ctgctccttt accgagggac tctggttaca
     2281 gcagctcctc ccctgggcag ttgttagata tactaaattc gaaaaagcct gacagcaacg
     2341 tgcaatcaag caaaaagcca aagcttaaaa tcttacagag aggaacggac ttgaattcaa
     2401 tcaagcaaaa caataatgat gaaactgctc attcaaactc tcaagctttg ctagatttgt
     2461 tgaaaaaacc aacatcatcg cagaagatac acgcttccaa accagatact tcctttttac
     2521 caaatgactc cgtatctggt atacaagatg cagaatatga agatttcgag agtagttcag
     2581 atgaagaggt ggagacagct agagatgaaa gaaattcatt gaatgtagat attggggtga
     2641 acgttatgcc aagcgaaaaa gacagccgaa gaagtcaaaa ggaaaaacca aggaacgacg
     2701 caagcaaaac aaacttgaac gcttctgcag aatctaatag tgtagaatgg gggcctggta
     2761 aatcttcacc ctcgactcag agtaaacaga attcatcagt aggaatgcag aataaatatc
     2821 gtcaagaaat tcacattggt gatagtgatg catatgaagt cttcgaaagc agttctgacg
     2881 aagaagatgg aaaaaaattg gaagaactgg aacaaactca agacaactcc aaattaatta
     2941 gccaagatat tttaaaagag aacaatttcc aagacggtga agtgcctcat agagatatgc
     3001 ccactgaaag taataaatct ataaacgaaa ctgtcgggtt atcatcaaca acaaatactg
     3061 taaaaaaggt acccaaagtt aaaatactaa aacgaggtga aacctttgcc agtctggcga
     3121 atgataaaaa agcttttgat tcaagctcca atgtgtcttc atcaaaagat ttattacaga
     3181 tgctaagaaa cccaatttcc tctactgtat cttcaaatca gcaatcacca aaatctcaac
     3241 atctaagtgg ggatgaagaa atcatgatga tgttgaagag gaattctgtg tcaaaacctc
     3301 aaaatagcga agaaaatgca tcaacttcaa gtataaacga tgcgaatgca agcgaacttt
     3361 tgggaatgtt aaaacaaaag gaaaaagaca ttacagcacc aaaacagccc tataatgttg
     3421 actcatactc gcagaaaaat tcagctaaag gattgttgaa tattttgaaa aagaacgact
     3481 ctaccgggta ccctcgcact gagggtggac cgtcaagtga aatgtcaacg tcaatgaaac
     3541 gcaacgacgc tacaaataat caagaattag ataagaattc aacagagtta ttaaactact
     3601 taaagcccaa gcctcttaat gatgggtatg agaatatttc aaataaggac agttctcatg
     3661 aattactgaa tatattgcat ggtaataaaa acagcagcgc cttcaataat aatgtatatg
     3721 caactgacgg atattccttg gcatctgata acaacgaaaa ttcctcaaat aaactactaa
     3781 atatgctgca aaacaggtct agtgcaataa atgagcccaa cttcgacgtc cgttcgaatg
     3841 gaacttcagg gtctaatgaa ttattaagca ttttgcatag gaagcggatc cccgggttaa
     3901 ttaacagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     3961 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     4021 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     4081 ttgtcactac tttcacttat ggtgttcaat gcttttcaag atacccagat catatgaaac
     4141 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     4201 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     4261 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     4321 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     4381 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     4441 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     4501 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     4561 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaatagg
     4621 gcgcgccact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
     4681 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     4741 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt
     4801 cgctcttatt gaccacacct ctaccggcag atccgctagg gataacaggg taatatagat
     4861 cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggg
     4921 cccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     4981 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     5041 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5101 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5161 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5221 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5281 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5341 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5401 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5461 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5521 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     5581 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac
     5641 tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat
     5701 acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg
     5761 taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata
     5821 ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg
     5881 tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct
     5941 cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca
     6001 atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca tgacaattct
     6061 gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg
     6121 ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt
     6181 ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg
     6241 tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc
     6301 tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct
     6361 aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag
     6421 tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta
     6481 gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt
     6541 gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg
     6601 cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt
     6661 accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat
     6721 gaattgaaaa ggtggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     6781 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6841 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6901 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6961 atgtcatgat aataatggtt tcttagacgg atcgcttgcc tgtaacttac acgcgcctcg
     7021 tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt
     7081 gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa
     7141 aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga
     7201 caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac
     7261 aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg
     7321 agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac
     7381 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat
     7441 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg
     7501 acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7561 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7621 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7681 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7741 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7801 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7861 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7921 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7981 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8041 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8101 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8161 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8221 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8281 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8341 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8401 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8461 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8521 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8581 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8641 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8701 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8761 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8821 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8881 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8941 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9001 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9061 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9121 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9181 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9241 ggagcctatg gaaa
//
