LOCUS       sequence-334281-       11219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS-CoV-2 soluble spike trimer protein 
            (B.1.351 variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R620-M12-303: CMV51p> SARS-CoV-2
            S(1-1208)-2P-...
FEATURES             Location/Qualifiers
     source          1..11219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(2030..2049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6541..6564
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6568..6591
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6598..6621
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6661..6684
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6686..6710)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6772..6790)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6773..6790)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6810..6827)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6816..7040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7086..7514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7173..7192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7383..7404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7523..7543)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7528..7857
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7708..7843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7770..7789
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7924..8718
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7978..7997)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8588..8607
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8892..9013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8929..8948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8983..9002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9062..9078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9062..9078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9075..9097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9086..9102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9110..9116,9117..9134,9135..9140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9155..9176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9293..9310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9464..10052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9544..9563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10223..11014,11015..11083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10846..10865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11084..11188)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcgtgtt ccttgtcctc ctgccgctgg tgtcatcgca gtgcgtgaac
     2881 ttcaccaccc ggactcagct gcctcctgct tacactaact ccttcactcg cggggtgtac
     2941 taccctgaca aagtgttccg gtcgtccgtg ctgcatagca cccaggacct gttccttccg
     3001 ttcttctcca acgtgacctg gttccacgcc attcacgtgt cggggaccaa cggcactaag
     3061 agattcgcca accccgtctt gcccttcaat gacggagtat acttcgcctc gacggaaaag
     3121 tccaacatca ttcggggttg gatcttcggc actaccctgg acagcaagac ccagtccctg
     3181 ctcattgtca acaacgccac caacgtcgtg atcaaagtct gcgagtttca gttttgtaac
     3241 gatccgttcc tcggcgtgta ctaccacaag aacaacaaga gctggatgga gtccgaattc
     3301 cgggtctact cctccgccaa caactgtacc ttcgaatacg tgtcccaacc tttcctcatg
     3361 gacctcgaag gaaaacaggg aaacttcaag aacttgagag agtttgtgtt caagaatatc
     3421 gatggctact tcaagatcta ctcgaagcat accccgatca acctcgtgcg gggactacct
     3481 caagggttct ccgcacttga gccgctcgtg gacctcccca ttggcatcaa catcaccaga
     3541 ttccagactc tgcatatctc gtatctcaca cccggggact cctcctccgg gtggacagct
     3601 ggtgctgcgg cctactacgt gggatatctc cagccccgga ccttcttgct gaagtacaac
     3661 gagaacggca ccatcacgga tgccgtggac tgtgcactgg accccttgag tgaaaccaag
     3721 tgcacgctga agtccttcac cgtggaaaaa ggcatctacc agaccagcaa cttcagggtg
     3781 cagcctactg aatcaatcgt gcggttccct aacattacca atctgtgccc gttcggcgaa
     3841 gtgttcaacg ccactagatt cgcctcggtg tacgcctgga accgaaagcg gatcagcaac
     3901 tgcgtggccg actactcagt gctctacaac tcggccagct tctccacctt caagtgctac
     3961 ggagtgtcgc ccaccaagct gaacgatctg tgcttcacca acgtgtacgc ggattccttc
     4021 gtgatccgcg gagatgaagt ccgccaaatc gcccccggtc aaaccggaaa cattgccgac
     4081 tacaactata agctgccgga cgactttacc ggatgcgtga ttgcctggaa ttccaacaat
     4141 ttggattcaa aagtcggcgg caactacaac tacctgtacc gcctgttccg gaagtcaaac
     4201 ctgaagccgt tcgaacgcga catcagcacc gaaatctatc aagcgggaag cactccctgc
     4261 aacggggtga agggcttcaa ctgctacttc ccgctgcaaa gctacggatt ccagcctacc
     4321 tacggcgttg gataccagcc atatcgcgtg gtcgtgctct cctttgaact tctgcacgcc
     4381 cctgccactg tgtgcggccc caagaagtcc actaacctcg tcaagaataa gtgcgtgaat
     4441 ttcaacttta acggattgac cgggaccgga gtgctcaccg agtccaacaa gaaattcctg
     4501 ccgttccaac agtttggacg ggacatcgcc gatactacgg atgctgtgcg ggacccacag
     4561 actctggaga tcctggacat tactccctgt tcctttggcg gggtgtcggt catcacccct
     4621 ggtactaaca ccagcaacca ggtcgccgtg ctgtatcagg gggtgaactg caccgaagtg
     4681 cccgtggcga tccacgccga ccagctgact ccaacttggc gcgtgtactc gactggttcg
     4741 aacgtgttcc agactcgggc cggttgcctg atcggagcgg agcatgtcaa caacagctac
     4801 gagtgcgaca tccctattgg agccgggatt tgcgccagct accagaccca gaccaattcg
     4861 cctgggtcgg ccagcagcgt ggcttcccaa tcgatcatcg cctacaccat gtccctggga
     4921 gtggagaact cagtggcgta ctccaacaac tcgattgcca tccccactaa tttcacgatc
     4981 agcgtgacca ccgagatcct gcctgtgtcc atgaccaaga cctccgtgga ctgcaccatg
     5041 tacatctgcg gagactccac cgagtgctcc aacctcctgc tgcaatacgg ctcgttctgt
     5101 acccaactga accgagccct gactgggatc gccgtggagc aggacaagaa cacacaggag
     5161 gtgttcgcac aagtcaagca gatctacaag accccgccca tcaaggattt cggagggttc
     5221 aatttctccc aaattctgcc ggacccttcc aagccgtcca agaggtcatt cattgaggat
     5281 ctcctgttca acaaggtcac cctggccgat gctggtttca tcaagcagta cggagactgc
     5341 ctgggggaca tcgcagcccg cgatctgatc tgcgcccaaa agttcaacgg cctgaccgtc
     5401 ctgcctccgc ttctgactga tgaaatgatt gcccagtaca cctctgcgct gctggctggc
     5461 actatcacct ccggctggac cttcggcgct ggagcagcac tgcagattcc atttgccatg
     5521 caaatggcct accgcttcaa cggaatcgga gtgactcaga acgtcctgta cgagaaccag
     5581 aagctcatcg ccaaccagtt caactccgcc attgggaaga ttcaggattc cctgtcgagc
     5641 actgcgtcag ccctgggaaa gctgcaggac gtcgtgaacc agaacgcaca ggccctgaac
     5701 acccttgtga agcagctgtc gtcgaacttc ggggcgatct cctccgtgtt gaacgacatc
     5761 ctgtcccggc tggacccgcc tgaggcagaa gtgcagatcg accggctgat taccggacgc
     5821 ctccagtccc ttcaaactta tgtgacccag cagctgattc gcgcggcgga aattcgggct
     5881 agcgccaatc tggctgccac caagatgagc gaatgcgtgc ttggacagtc caagagagtg
     5941 gacttttgtg gaaagggcta ccacctgatg agcttccccc aatccgcccc acacggtgtc
     6001 gtgtttctgc acgtgaccta cgtgccggcg caggaaaaga acttcaccac tgcccctgcc
     6061 atctgccacg atgggaaggc gcacttcccg agggagggag tgttcgtgtc aaacggaacc
     6121 cactggttcg tgacccaaag gaacttctac gagccccaga ttatcaccac cgataacact
     6181 ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaacactgt gtacgacccg
     6241 ctgcaaccgg agctcgatag ctttaaggaa gaactcgaca agtactttaa gaatcatacg
     6301 tcccccgatg tggatctggg cgacattagc ggtatcaacg cgtctgtcgt gaacattcag
     6361 aaggagattg acaggctcaa cgaagtcgcc aaaaacctga acgagagcct gatagacttg
     6421 caggaactgg gaaaatacga gcagggctcc ggttacatcc ccgaagctcc acgggacgga
     6481 caggcctacg tgcgcaagga cggagaatgg gtgctgctga gcaccttcct cgggcggtcc
     6541 ctggaggtgc tgttccaagg cccaggccac catcatcatc accaccacca ctcggcttgg
     6601 tcacaccccc aattcgaaaa gggcggtggt tctggaggag gcggatccgg aggatccgcg
     6661 tggagtcatc cgcagtttga aaagtaatca actttcttgt acaaagttgt gaaattctgc
     6721 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccta ttctatagtg
     6781 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6841 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6901 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6961 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7021 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7081 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7141 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7201 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7261 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7321 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7381 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7441 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7501 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     7561 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     7621 aagtccctag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7681 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7741 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     7801 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7861 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     7921 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7981 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     8041 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     8101 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     8161 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     8221 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     8281 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8341 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8401 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     8461 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8521 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8581 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8641 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8701 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     8761 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8821 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8881 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8941 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9001 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     9061 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9121 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9181 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9901 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
