LOCUS       sequence-334298-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-A-SP44-mScarlet-I
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(39..82)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    85..104
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     812..835
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tttgaattac ctataaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
       61 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagaacgtc tcaatctcta
      121 ttgttcacat tcgaaccgtc tctgctttga caacatgctg tgcggtgttg taaagtctgg
      181 tgtagtactt taactttaag aaggagatat acacatatgg tgtccaaggg cgaggccgtc
      241 atcaaggagt tcatgcgctt caaggtccac atggagggct ccatgaacgg ccacgagttc
      301 gagatcgagg gcgagggcga gggccgcccg tacgagggca cccagaccgc caagctgaag
      361 gtcaccaagg gcggcccgct gccgttctcc tgggacatcc tgtccccgca gttcatgtac
      421 ggctcccgcg ccttcatcaa gcacccggcc gacatcccgg actactacaa gcagtccttc
      481 ccggagggct tcaagtggga gcgcgtcatg aacttcgagg acggcggcgc cgtcaccgtc
      541 acccaggaca cctccctgga ggacggcacc ctgatctaca aggtcaagct gcgcggcacc
      601 aacttcccgc cggacggccc ggtcatgcag aagaagacca tgggctggga ggcctccacc
      661 gagcgcctgt acccggagga cggcgtcctg aagggcgaca tcaagatggc cctgcgcctg
      721 aaggacggcg gccgctacct ggccgacttc aagaccacct acaaggccaa gaagccggtc
      781 cagatgccgg gcgcctacaa cgtcgaccgc aagctggaca tcacctccca caacgaggac
      841 tacaccgtcg tcgagcagta cgagcgctcc gagggccgcc actccaccgg cggcatggac
      901 gagctgtaca agggatccga ctacaaggac gacgacgac
//
