LOCUS       sequence-334301-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-A-SP44-sfGFP
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      685..756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      772..799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctggacggcg acgtgaacgg ccacaagttc tccgtccgcg gcgagggcga gggcgacgcc
       61 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctccc ggtcccgtgg
      121 ccgaccctgg tcaccaccct gacctacggc gtccagtgct tctcccgcta cccggaccac
      181 atgaagcgcc acgacttctt caagtccgcc atgcccgagg gctacgtcca ggagcggacc
      241 atctccttca aggacgacgg cacctacaag acccgcgccg aggtcaagtt cgagggcgac
      301 accctggtca accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      361 ggccacaagc tcgagtacaa cttcaactcc cacaacgtct acatcaccgc cgacaagcag
      421 aagaacggca tcaaggccaa cttcaagatc cgccacaacg tcgaggacgg cagcgtccag
      481 ctggccgacc actaccagca gaacaccccg atcggcgacg gcccggtcct gctgccggac
      541 aaccactacc tgtccaccca gtccgtcctg tccaaggacc cgaacgagaa gcgcgaccac
      601 atggtcctgc tcgagttcgt caccgccgcc ggcatcaccc acggcatgga cgagctgtac
      661 aagtgaggat cctcgaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      721 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      781 tcgggtgggc ctttctgcgt ttatatgttt gccagagacg ctgcag
//
