LOCUS       sequence-334302-        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-A-SP44-sfGFP
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(39..82)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    85..104
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             join(215..217,218..931)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(257..278)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      950..1021
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1037..1064
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1097..1114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttcgaattac ctataaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
       61 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagaacgtc tcaatctcta
      121 ttgttcacat tcgaaccgtc tctgctttga caacatgctg tgcggtgttg taaagtctgg
      181 tgtagtactt taactttaag aaggagatat acatatgtcc aagggcgagg agctgttcac
      241 cggcgtcgtc ccgatcctgg tcgagctgga cggcgacgtg aacggccaca agttctccgt
      301 ccgcggcgag ggcgagggcg acgccaccaa cggcaagctg accctgaagt tcatctgcac
      361 caccggcaag ctcccggtcc cgtggccgac cctggtcacc accctgacct acggcgtcca
      421 gtgcttctcc cgctacccgg accacatgaa gcgccacgac ttcttcaagt ccgccatgcc
      481 cgagggctac gtccaggagc ggaccatctc cttcaaggac gacggcacct acaagacccg
      541 cgccgaggtc aagttcgagg gcgacaccct ggtcaaccgc atcgagctga agggcatcga
      601 cttcaaggag gacggcaaca tcctgggcca caagctcgag tacaacttca actcccacaa
      661 cgtctacatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      721 caacgtcgag gacggcagcg tccagctggc cgaccactac cagcagaaca ccccgatcgg
      781 cgacggcccg gtcctgctgc cggacaacca ctacctgtcc acccagtccg tcctgtccaa
      841 ggacccgaac gagaagcgcg accacatggt cctgctcgag ttcgtcaccg ccgccggcat
      901 cacccacggc atggacgagc tgtacaagtg aggatcctcg accaggcatc aaataaaacg
      961 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1021 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tgtttgccag
     1081 agacgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ttgcggcgag
     1141 cgg
//
