LOCUS       sequence-33432-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Fan W, Nassiri A, Zhong Q
  TITLE     Autophagosome targeting and membrane curvature sensing by 
            Barkor/Atg14(L).
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7769-74. doi: 
            10.1073/pnas.1016472108. Epub 2011 Apr 25.
  PUBMED    21518905
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1016472108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7769-74"
COMMENT     Sequence Label: hrGFP-BATS
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             367..432
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(486..505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(487..505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    779..900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(816..835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     870..889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agaggcggct tcgtggagca gcacgagacc gccatcgccc agctgaccag cctgggcaag
       61 cccctgggca gcctgcacga gtgggtggag ctctccggac tcagatctgc tggagaatca
      121 gatgagagcg gagatgagcg cgtcagcgat gaagaaaccg acctgggcac agactgggag
      181 aacttgccta gtccccggtt ttgtgatatc ccttcccagt ctgtggaagt ctcccagagt
      241 cagagcaccc aggcgtcccc acccatcgcg agcagcagtg caggtgggat gatctcctct
      301 gcagcagcct cggtgacctc ctggtttaaa gcttacactg gacaccgttc gaattctgca
      361 gtcgacgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
      421 gacgatgaca agtagggatc caccggatct agataatagg gtaccaggta agtgtaccca
      481 attcgcccta tagtgagtcg tattacaatt cactcgatcg cccttcccaa cagttgcgca
      541 gcctgaatgg cgaatggaga tccaattttt aagtgtataa tgtgttaaac tactgattct
      601 aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca
      661 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      721 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
      781 cttgtttatt gcagcttata atggttacaa atnaagcaat agcatcacaa atttcacaaa
      841 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      901 acgcgtaatt gtaagcgtta tatttgt
//
