LOCUS       sequence-334322-        5912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Component of the Co-Driver binary site-specific recombinase system, 
            expression of the site-specific recombinase Dre (mouse 
            codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-NLS-HA-Dre
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..665
                     /label=chicken beta-actin promoter
     intron          666..1682
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1606..1628
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1695..1714
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1695..1713
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1733..1753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1754..1780
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1787..2809
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     primer_bind     complement(2832..2849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2838..3062
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3134..3151)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3134..3150)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3143..3165)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3291..3746)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3428..3449)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3640..3659
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3773..3877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3878..3946,3947..4738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4096..4115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4909..5497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5398..5417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5651..5668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5785..5806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5821..5826,5827..5844,5845..5851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5859..5875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5864..5886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5883..5899
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5883..5899
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggattcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
      421 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
      481 cagcgatggg ggcggggggg gggggggggc gcgcgccrgg sggggsgggg sggggsgrgg
      541 ggsggggsgg ggsgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
      721 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
      781 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
      841 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
      961 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1021 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     1081 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     1141 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     1201 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     1261 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     1321 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     1381 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     1441 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     1561 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1621 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1681 agatccttaa ttaataatac gactcactat agggggtcga cccgccacca tgcctaagaa
     1741 gaagaggaag gtttacccat acgatgttcc agattacgct ggatcctctg agctgattat
     1801 tagtggttca tctggtggat tcctgcgaaa catcggcaaa gagtatcagg aggccgctga
     1861 aaacttcatg aggtttatga atgaccaggg ggcgtacgct cctaacactt tgagggattt
     1921 gaggttggtc tttcatagct gggccagatg gtgccatgct cggcagcttg catggtttcc
     1981 aattagtcct gaaatggcac gcgaatactt tcttcagttg cacgatgcag acctggcctc
     2041 cactaccatc gacaagcact atgctatgct taatatgctt ctgtcccact gcggactgcc
     2101 acccttgtcc gacgacaagt cagtgagtct tgccatgaga agaattagaa gagaagccgc
     2161 aaccgaaaag ggtgagagga caggacaggc aatccccctg cgctgggacg acctgaagct
     2221 gctggatgtg ctgctcagca ggagcgagcg gctggtcgac ctgcgcaaca gggctttcct
     2281 gttcgtagcc tataacaccc tcatgagaat gtctgaaata tcacgcatca gggttgggga
     2341 cttggatcag acaggagaca cagtgaccct gcacatcagt cacactaaga caatcaccac
     2401 agctgcgggc cttgacaaag tgctctcccg gcgaaccaca gcagtgctca atgactggct
     2461 ggacgtcagt gggcttagag aacatccaga cgctgtgctc ttcccaccta tacaccggtc
     2521 aaacaaagcc cgcattacta ccacgcccct gaccgcccct gccatggaga agattttcag
     2581 tgatgcctgg gtgctgctga acaaacggga cgccaccccc aataaaggga ggtataggac
     2641 ctggaccggc cattccgcca gggtgggtgc cgcaatagac atggccgaga aacaggtgtc
     2701 tatggtcgag attatgcagg aagggacatg gaagaagcct gaaacactga tgcggtatct
     2761 cagaaggggc ggagtgtccg tgggagccaa ttctcgactg atggatagct gagtcagagc
     2821 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2881 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2941 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3001 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3061 ggcttctgag gcggaaagaa ccagctgggg ctcgagatcc actagttcta gcctcgaggc
     3121 tagagcggcc gccactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3181 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3241 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag
     3301 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3361 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3421 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3481 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3541 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3601 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3661 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3721 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     3781 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3901 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3961 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4021 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4081 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4141 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4201 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4261 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4321 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4381 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4441 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4501 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4561 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4621 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4681 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4741 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5641 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5701 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5761 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5821 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5881 cacaggaaac agctatgacc atgaggcgcg cc
//
