LOCUS       sequence-334325-        3509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TESEC expression vector for TrpD from M. tuberculosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Bongaerts N, Edoo Z, Abukar AA, Song X, Sosa-Carrillo S, 
            Haggenmueller S, Savigny J, Gontier S, Lindner AB, Wintermute EH
  TITLE     Low-cost anti-mycobacterial drug discovery using engineered E. coli.
  JOURNAL   Nat Commun. 2022 Jul 7;13(1):3905. doi: 10.1038/s41467-022-31570-3.
  PUBMED    35798732
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-7"; volume: "13(1):3905. doi"; pages: " 
            10.1038/s41467-022-31570-3"
COMMENT     Sequence Label: pNB208
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..291
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     214..233
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             302..313
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1468..1554
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1719..2307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1799..1818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2395..2489)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2519..3310,3311..3379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3142..3161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3380..3484)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
       61 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
      121 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
      181 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
      241 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tagaattcat
      301 taaagaggag aaaggtacca tggcattatc cgctgagggc tcatctggag gctctcgcgg
      361 cggtagcccg aaggcggaag cggcgtccgt accatcgtgg ccgcaaatct tggggcgcct
      421 taccgataat cgtgatttag cgcgcgggca ggcggcatgg gccatggatc aaattatgac
      481 cggaaacgca cgccctgccc aaattgctgc gttcgcggtc gccatgacga tgaaagcccc
      541 aaccgctgat gaggtagggg agttggcagg tgtaatgttg agccatgcac accctctgcc
      601 cgctgacact gtccctgacg atgcagtcga tgtcgtggga actggcgggg atggggtcaa
      661 tacagtgaac ttatcgacca tggcagcgat tgtcgtggcc gcagcaggcg ttccagttgt
      721 gaagcacggc aaccgtgctg cgtccagctt aagtgggggg gcagatacat tggaagccct
      781 gggtgtccgc attgatttgg ggccggacct ggtcgcacgc tcacttgctg aggtgggaat
      841 tggcttctgc ttcgcacccc gcttccatcc ctcatatcgc catgctgcgg cagtccgtcg
      901 cgagatcggc gtaccaacag tctttaatct gcttggacct ctgacaaatc cggcacgtcc
      961 acgcgcggga ctgatcggtt gtgcgttcgc agatttagcg gaggttatgg caggtgtatt
     1021 cgccgcgcgt cgcagctccg tccttgttgt gcatggggat gatgggcttg atgagttaac
     1081 gaccacgact accagtacta tttggcgtgt cgcggcagga tctgtggata aactgacctt
     1141 cgacccagca ggattcggat ttgctcgtgc acaattggat caattggctg gtggggacgc
     1201 ccaggctaac gcagctgccg tgcgcgcggt cttgggcggg gctcgtggac ccgtgcgtga
     1261 tgcagtcgtg ctgaatgcag caggcgcgat tgtagcccat gcaggcctta gtagccgtgc
     1321 ggagtggttg ccggcttggg aggaaggcct gcgccgcgcc tctgctgcta tcgacacagg
     1381 tgctgcggaa caattgctgg cacgctgggt ccgtttcgga cgccagatct gacccggggg
     1441 atcccatggt acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1501 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1561 ccctagacct agggcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgactagtgc ttggattctc
     2401 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     2461 ggtcattact ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt
     2521 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2581 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2641 gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc
     2701 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2761 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2821 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2881 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2941 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3001 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3061 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3481 cgcgcacatt tccccgaaaa gtgccacct
//
