LOCUS       sequence-334326-        3161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TESEC expression vector for CysH from M. tuberculosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Bongaerts N, Edoo Z, Abukar AA, Song X, Sosa-Carrillo S, 
            Haggenmueller S, Savigny J, Gontier S, Lindner AB, Wintermute EH
  TITLE     Low-cost anti-mycobacterial drug discovery using engineered E. coli.
  JOURNAL   Nat Commun. 2022 Jul 7;13(1):3905. doi: 10.1038/s41467-022-31570-3.
  PUBMED    35798732
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-7"; volume: "13(1):3905. doi"; pages: " 
            10.1038/s41467-022-31570-3"
COMMENT     Sequence Label: pNB206
FEATURES             Location/Qualifiers
     source          1..3161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..291
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     214..233
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             302..313
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1120..1206
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1371..1959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1451..1470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2047..2141)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2171..2962,2963..3031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2794..2813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3032..3136)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
       61 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
      121 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
      181 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
      241 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tagaattcat
      301 taaagaggag aaaggtacca tgtcaggtga aacaacgcgc ttaacggagc cacagctgcg
      361 tgaactggcg gcgcgtggcg cagcggagct ggacggtgca acggcaacag atatgctgcg
      421 ttggacggat gaaaccttcg gcgatattgg cggtgcaggg ggcggcgtaa gcggtcaccg
      481 cggttggact acgtgcaatt acgttgttgc ttcgaacatg gccgatgctg ttctggtcga
      541 cctggcggcg aaagttcgcc ctggcgtacc ggttatcttt ctggataccg ggtatcattt
      601 tgttgaaacc atcgggactc gtgacgcgat cgaaagcgtt tacgatgtac gcgtgttaaa
      661 cgtcacccca gaacatacgg tggcggaaca ggatgagctg cttggcaaag atctgttcgc
      721 gcgcaacccc catgaatgtt gccgcttgcg caaagtggtt ccgttaggta aaaccttgcg
      781 cggttattcc gcttgggtaa ctgggctgcg tcgcgtggac gccccgacgc gtgcgaatgc
      841 tcctctggtt agctttgatg aaacctttaa actggtgaaa gtaaatcccc tggcggcgtg
      901 gaccgaccag gatgtgcaag aatatatcgc cgataacgat gtcctcgtaa acccactggt
      961 tcgtgaaggg tatccatcaa tcggctgtgc gccgtgtacc gcaaagccgg ccgaaggtgc
     1021 ggatccgcgc agcggccgct ggcaagggct ggcaaaaacg gaatgcggtc ttcacgcttc
     1081 gtaacccggg ggatcccatg gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag
     1141 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1201 acaaatccgc cgccctagac ctagggcgtt cggctgcggc gagcggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgactagt
     2041 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2101 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgtaaactt
     2161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2281 catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat
     2341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc t
//
