LOCUS       sequence-334337-        3124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TESEC expression vector for mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Bongaerts N, Edoo Z, Abukar AA, Song X, Sosa-Carrillo S, 
            Haggenmueller S, Savigny J, Gontier S, Lindner AB, Wintermute EH
  TITLE     Low-cost anti-mycobacterial drug discovery using engineered E. coli.
  JOURNAL   Nat Commun. 2022 Jul 7;13(1):3905. doi: 10.1038/s41467-022-31570-3.
  PUBMED    35798732
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-7"; volume: "13(1):3905. doi"; pages: " 
            10.1038/s41467-022-31570-3"
COMMENT     Sequence Label: pNB202
FEATURES             Location/Qualifiers
     source          1..3124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..289
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     212..231
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             300..311
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(315..317,318..320,321..1034)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(360..381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(621..640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     968..989
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      1069..1155
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1320..1908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1400..1419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1996..2090)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2120..2911,2912..2980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2743..2762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2981..3085)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
       61 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
      121 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
      181 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
      241 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata gaattcatta
      301 aagaggagaa aggaatggtt agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      361 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      421 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      481 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      541 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      601 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      661 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      721 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      781 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      841 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      901 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      961 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1021 agctgtacaa gtaaaggtgg gatcccatgg tacgcgtgct agaggcatca aataaaacga
     1081 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1141 ctgagtagga caaatccgcc gccctagacc tagggcgttc ggctgcggcg agcggtatca
     1201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1501 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1621 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1681 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1741 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1801 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1861 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1921 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1981 atgactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2041 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2101 cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2161 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2221 agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc
     2281 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2341 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2401 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2461 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2521 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2581 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2641 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2701 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2761 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2821 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2881 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2941 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3001 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3061 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgaag
     3121 acat
//
