LOCUS       sequence-334338-        3623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TESEC expression vector for Alr from M. tuberculosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Bongaerts N, Edoo Z, Abukar AA, Song X, Sosa-Carrillo S, 
            Haggenmueller S, Savigny J, Gontier S, Lindner AB, Wintermute EH
  TITLE     Low-cost anti-mycobacterial drug discovery using engineered E. coli.
  JOURNAL   Nat Commun. 2022 Jul 7;13(1):3905. doi: 10.1038/s41467-022-31570-3.
  PUBMED    35798732
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-7"; volume: "13(1):3905. doi"; pages: " 
            10.1038/s41467-022-31570-3"
COMMENT     Sequence Label: pNB201
FEATURES             Location/Qualifiers
     source          1..3623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..291
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     214..233
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             302..313
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1582..1668
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1833..2421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1913..1932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2509..2603)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2633..3424,3425..3493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3256..3275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3494..3598)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
       61 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
      121 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
      181 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
      241 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tagaattcat
      301 taaagaggag aaaggtacca tgaaacgctt ttgggaaaat gtgggtaaac ctaacgatac
      361 gacggacggt cgcggcacca cctccctcgc gatgacaccg atctcacaga ctccgggctt
      421 actggccgag gcgatggttg acctcggagc tattgaacat aatgtgcgtg tcctccgcga
      481 acatgcgggc cacgcccagt tgatggcagt cgttaaggca gacggctacg gtcacggggc
      541 tacacgcgta gctcaaacgg ccttaggcgc cggtgctgcg gaactggggg tcgcgacagt
      601 ggatgaagct cttgcgctgc gtgccgacgg cattaccgcg ccggtattag cgtggctgca
      661 tccgccgggt atcgactttg gtccagccct gctggccgat gttcaggtgg cggtttcttc
      721 actgcgccag ttggatgaat tgctgcacgc ggttcgccgt accggtcgca ccgcgaccgt
      781 cacggtcaaa gtggataccg gactcaaccg caacggcgta ggtccagcac aattcccagc
      841 gatgttaacg gcgcttcgtc aggccatggc tgaagatgct gtgcgcctgc gcggcctgat
      901 gagccatatg gtttatgcag ataagcccga cgatagtatt aatgacgtac aagcgcaacg
      961 ttttactgct ttcctggcgc aggcgcgtga acagggcgtg cgttttgaag tggcacatct
     1021 gtcaaacagt tcggccacga tggcccgccc agacctgaca ttcgatctgg tgcgccctgg
     1081 tatcgctgtc tacgggcttt caccggtgcc ggccctgggt gatatgggcc tggtgccggc
     1141 aatgacagtc aaatgtgccg tggcgctcgt gaaaagtatt cgtgcgggtg aaggcgtgtc
     1201 ttatgggcat acatggatcg caccccgcga tactaatctg gcgctgttgc cgattggcta
     1261 tgccgatggc gtttttcgct cattaggcgg acgtctggaa gttctgatta atggacgccg
     1321 ctgcccaggt gtaggtcgta tttgtatgga tcagtttatg gtcgatctgg gtccgggccc
     1381 gctggacgtc gcggagggtg acgaagcgat cctgtttggt ccgggcatcc gcggagaacc
     1441 gaccgcccaa gactgggcgg acctggtggg gactattcac tacgaagtgg taacctcacc
     1501 gcgcgggcgt attacccgta cgtaccgcga agctgaaaac cgctaacccg ggggatccca
     1561 tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1621 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
     1681 acctagggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1741 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2281 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2341 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2401 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2461 tggaacgaaa actcacgtta agggattttg gtcatgacta gtgcttggat tctcaccaat
     2521 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     2581 tactggatct atcaacagga gtccaagcga gctcgtaaac ttggtctgac agttaccaat
     2641 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2701 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2761 caatgatacc gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2821 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2881 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2941 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3001 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3061 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3121 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3181 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3241 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3301 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3361 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3421 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3481 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3541 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3601 catttccccg aaaagtgcca cct
//
