LOCUS       sequence-334359-       11993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses catalytically-active GFP-cGAS after lentiviral 
            transduction. Can be used to monitor nuclear envelope rupture 
            events..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Raab M, Gentili M, de Belly H, Thiam HR, Vargas P, Jimenez AJ, 
            Lautenschlaeger F, Voituriez R, Lennon-Dumenil AM, Manel N, Piel M
  TITLE     ESCRT III repairs nuclear envelope ruptures during cell migration to
            limit DNA damage and cell death.
  JOURNAL   Science. 2016 Apr 15;352(6283):359-62. doi: 10.1126/science.aad7611.
            Epub 2016 Mar 24.
  PUBMED    27013426
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad7611"; journalName: "Science"; date: 
            "2016-04-15- 15"; volume: "352"; issue: "6283"; pages: "359-62"
COMMENT     Sequence Label: pTRIP-CMV-GFP-FLAG-cGAS
FEATURES             Location/Qualifiers
     source          1..11993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3254..3557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3558..3761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3712..3732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3807..3816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3813..3815,3816..3818,3819..4529)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3813..3815,3816..3818,3819..4529)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3858..3879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4119..4138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4466..4487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4536..4559
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6397..6577
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8105..8124)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8338..8357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8457..8479
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8517..8535)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8603..8707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8708..8776,8777..9568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8926..8945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9739..10327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10228..10247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10481..10498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10568..10588)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10573..10902
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10753..10888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtatagtaat caattacggg gtcattagtt catagcccat
     3241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     3781 tcccgcgggc tagcggatct gtcgacgcca ccatggtgag caagggcgag gagctgttca
     3841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4021 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4261 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4501 tcactctcgg catggacgag ctgtacaaga ctagtgacta caaagacgat gacgacaaga
     4561 tgcagccttg gcacggaaag gccatgcaga gagcttccga ggccggagcc actgccccca
     4621 aggcttccgc acggaatgcc aggggcgccc cgatggatcc caacgagtct ccggctgccc
     4681 ccgaggccgc cctgcctaag gcgggaaagt tcggccccgc caggaagtcg ggatcccggc
     4741 agaaaaagag cgccccggac acccaggaga ggccgcccgt ccgcgcaact ggggcccgcg
     4801 ccaaaaaggc ccctcagcgc gcccaggaca cgcagccgtc tgacgccacc agcgcccctg
     4861 gggcagaggg gctggagcct cctgcggctc gggagccggc tctttccagg gctggttctt
     4921 gccgccagag gggcgcgcgc tgctccacga agccaagacc cccgcccggg ccctgggacg
     4981 tgcccagccc cggcctgccg gtctcggccc ccattctcgt acggagggat gcggcgcctg
     5041 gggcctcgaa gctccgggcg gttttggaga agttgaagct cagccgcgat gatatctcca
     5101 cggcggcggg gatggtgaaa ggggttgtgg accacctgct gctcagactg aagtgcgact
     5161 ccgcgttcag aggcgtcggg ctgctgaaca ccgggagcta ctatgagcac gtgaagattt
     5221 ctgcacctaa tgaatttgat gtcatgttta aactggaagt ccccagaatt caactagaag
     5281 aatattccaa cactcgtgca tattactttg tgaaatttaa aagaaatccg aaagaaaatc
     5341 atctgagtca gtttttagaa ggtgaaatat tatcagcttc taagatgctg tcaaagttta
     5401 ggaaaatcat taaggaagaa attaacgaca ttaaagatac agatgtcatc atgaagagga
     5461 aaagaggagg gagccctgct gtaacacttc ttattagtga aaaaatatct gtggatataa
     5521 ccctggcttt ggaatcaaaa agtagctggc ctgctagcac ccaagaaggc ctgcgcattc
     5581 aaaactggct ttcagcaaaa gttaggaagc aactacgact aaagccattt taccttgtac
     5641 ccaagcatgc aaaggaagga aatggtttcc aagaagaaac atggcggcta tccttctctc
     5701 acatcgaaaa ggaaattttg aacaatcatg gaaaatctaa aacgtgctgt gaaaacaaag
     5761 aagagaaatg ttgcaggaaa gattgtttaa aactaatgaa atacctttta gaacagctga
     5821 aagaaaggtt taaagacaaa aaacatctgg ataaattctc ttcttatcat gtgaaaactg
     5881 ccttctttca cgtatgtacc cagaaccctc aagacagtca gtgggaccgc aaagacctgg
     5941 gcctctgctt tgataactgc gtgacatact ttcttcagtg cctcaggaca gaaaaacttg
     6001 agaattattt tattcctgaa ttcaatctat tctctagcaa cttaattgac aaaagaagta
     6061 aagaatttct gacaaagcaa attgaatatg aaagaaacaa tgagtttcca gtttttgatg
     6121 aattttgact cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     6181 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     6241 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6301 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaaa atcgtcgaga
     6361 gatgctgcat ataagcagct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6421 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6481 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6541 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6601 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattat
     6661 aatagattta gcaggaattg aactaggagt ggagcacaca ggcaaagctg cagaagtact
     6721 tggaagaagc caccagagat actcacgatt ctgcacatac ctggctaatc ccagatccta
     6781 aggattacat taagtttact aacatttata taatgattta tagtttaaag tataaactta
     6841 tctaatttac tattctgaca gatattaatt aatcctcaaa tatcataaga gatgattact
     6901 attatcccca tttaacacaa gaggaaactg agagggaaag atgttgaagt aattttccca
     6961 caattacagc atccgttagt tacgactcta tgatcttctg acacaaattc catttactcc
     7021 tcaccctatg actcagtcga atatatcaaa gttatggaca ttatgctaag taacaaatta
     7081 cccttttata tagtaaatac tgagtagatt gagagaagaa attgtttgca aacctgaata
     7141 gcttcaagaa gaagagaagt gaggataaga ataacagttg tcatttaaca agttttaaca
     7201 agtaacttgg ttagaaaggg attcaaatgc ataaagcaag ggataaattt ttctggcaac
     7261 aagactatac aatataacct taaatatgac ttcaaataat tgttggaact tgataaaact
     7321 aattaaatat tattgaagat tatcaatatt ataaatgtaa tttactttta aaaagggaac
     7381 atagaaatgt gtatcattag agtagaaaac aatccttatt atcacaattt gtcaaaacaa
     7441 gtttgttatt aacacaagta gaatactgca ttcaattaag ttgactgcag attttgtgtt
     7501 ttgttaaaat tagaaagaga taacaacaat ttgaattatt gaaagtaaca tgtaaatagt
     7561 tctacatacg ttcttttgac atcttgttca atcattgatc gaagttcttt atcttggaag
     7621 aatttgttcc aaagactctg aaataaggaa aacaatctat tatatagtct cacacctttg
     7681 ttttactttt agtgatttca atttaataat gtaaatggtt aaaatttatt cttctctgag
     7741 atcatttcac attgcagata gaaaacctga gactggggta atttttatta aaatctaatt
     7801 taatctcaga aacacatctt tattctaaca tcaatttttc cagtttgata ttatcatata
     7861 aagtcagcct tcctcatctg caggttccac aacaaaaatc caaccaactg tggatcaaaa
     7921 atattgggaa aaaattaaaa atagcaatac aacaataaaa aaatacaaat cagaaaaaca
     7981 gcacagtata acaactttat ttagcattta caatctatta ggtattataa gtaatctagc
     8041 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     8101 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     8161 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     8221 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     8281 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     8341 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     8401 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8461 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     8521 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8581 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8641 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8701 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8761 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8821 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8881 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8941 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9001 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9061 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9121 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9181 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9241 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9301 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9361 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9421 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9481 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9541 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9601 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9661 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9721 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9781 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9841 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9901 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9961 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10021 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10081 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10141 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10201 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10261 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10321 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10381 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10441 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10501 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10561 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10621 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10681 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10741 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10801 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10861 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
    10921 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
    10981 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
    11041 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
    11101 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
    11161 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
    11221 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
    11281 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
    11341 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
    11401 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
    11461 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
    11521 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
    11581 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
    11641 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
    11701 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
    11761 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
    11821 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
    11881 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
    11941 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtg
//
