LOCUS       sequence-334360-       10064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector for human direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Zhou Y, Liu Z, Welch JD, Gao X, Wang L, Garbutt T, Keepers B, Ma H, 
            Prins JF, Shen W, Liu J, Qian L
  TITLE     Single-Cell Transcriptomic Analyses of Cell Fate Transitions during 
            Human Cardiac Reprogramming.
  JOURNAL   Cell Stem Cell. 2019 Jul 3;25(1):149-164.e9. doi: 
            10.1016/j.stem.2019.05.020. Epub 2019 Jun 20.
  PUBMED    31230860
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.05.020"; journalName: "Cell Stem Cell"; date: 
            "2019-07-3- 3"; volume: "25"; issue: "1"; pages: "149-164.e9"
COMMENT     Sequence Label: pMXs-puro-hMGT
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1073..1489
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1419..1441
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1457..1473
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1499..1873
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1811..1827
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             3324..3380
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4728..4781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6378..6398)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6383..6712
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6563..6698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6722..7321
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6722..6741)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7218..7238
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7548..8017
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8147..8164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8318..8906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8398..8417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9070..9861,9862..9930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9693..9712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9931..10035)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
      901 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     1081 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1141 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1201 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1261 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1321 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1381 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1441 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     1501 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1561 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1621 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     1681 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     1741 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     1801 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     1861 atcctctaga ctgccggatc tagctagtta attaaggatc cgttaacatc gataccgcca
     1921 tggggagaaa aaagattcag attacgagga ttatggatga acgtaacaga caggtgacat
     1981 ttacaaagag gaaatttggg ttgatgaaga aggcttatga gctgagcgtg ctgtgtgact
     2041 gtgagattgc gctgatcatc ttcaacagca ccaacaagct gttccagtat gccagcaccg
     2101 acatggacaa agtgcttctc aagtacacgg agtacaacga gccgcatgag agccggacaa
     2161 actcagacat cgtggaggca ttgaacaaga aagaaaacaa aggctgtgaa agccccgatc
     2221 ccgactcctc ttatgcactc accccacgca ctgaagaaaa atacaaaaaa attaatgaag
     2281 aatttgataa tatgatcaag agtcataaaa ttcctgctgt tccacctccc aacttcgaga
     2341 tgccagtctc catcccagtg tccagccaca acagtttggt gtacagcaac cctgtcagct
     2401 cactgggaaa ccccaaccta ttgccactgg ctcacccttc tctgcagagg aatagtatgt
     2461 ctcctggtgt aacacatcga cctccaagtg caggtaacac aggtggtctg atgggtggag
     2521 acctcacgtc tggtgcaggc accagtgcag ggaacgggta tggcaatccc cgaaactcac
     2581 caggtctgct ggtctcacct ggtaacttga acaagaatat gcaagcaaaa tctcctcccc
     2641 caatgaattt aggaatgaat aaccgtaaac cagatctccg agttcttatt ccaccaggca
     2701 gcaagaatac gatgccatca gtgaatcaaa ggataaataa ctcccagtcg gctcagtcat
     2761 tggctacccc agtggtttcc gtagcaactc ctactttacc aggacaagga atgggaggat
     2821 atccatcagc catttcaaca acatatggta ccgagtactc tctgagtagt gcagacctgt
     2881 catctctgtc tgggtttaac accgccagcg ctcttcacct tggttcagta actggctggc
     2941 aacagcaaca cctacataac atgccaccat ctgccctcag tcagttggga gcttgcacta
     3001 gcactcattt atctcagagt tcaaatctct ccctgccttc tactcaaagc ctcaacatca
     3061 agtcagaacc tgtttctcct cctagagacc gtaccaccac cccttcgaga tacccacaac
     3121 acacgcgcca cgaggcgggg agatctcctg ttgacagctt gagcagctgt agcagttcgt
     3181 acgacgggag cgaccgagag gatcaccgga acgaattcca ctcccccatt ggactcacca
     3241 gaccttcgcc ggacgaaagg gaaagtccct cagtcaagcg catgcgactt tctgaaggat
     3301 gggcaacagc tagcggcagc ggcgccacaa acttctctct gctaaagcaa gcaggtgatg
     3361 ttgaagaaaa ccccgggcct actagtatgt atcagagctt ggccatggcc gccaaccacg
     3421 ggccgccccc cggtgcctac gaggcgggcg gccccggcgc cttcatgcac ggcgcgggcg
     3481 ccgcgtcctc gccagtctac gtgcccacac cgcgggtgcc ctcctccgtg ctgggcctgt
     3541 cctacctcca gggcggaggc gcgggctctg cgtccggagg cgcctcgggc ggcagctccg
     3601 gtggggccgc gtctggtgcg gggcccggga cccagcaggg cagcccggga tggagccagg
     3661 cgggagccga cggagccgct tacaccccgc cgccggtgtc gccgcgcttc tccttcccgg
     3721 ggaccaccgg gtccctggcg gccgccgccg ccgctgccgc ggcccgggaa gctgcggcct
     3781 acagcagtgg cggcggagcg gcgggtgcgg gcctggcggg ccgcgagcag tacgggcgcg
     3841 ccggcttcgc gggctcctac tccagcccct acccggctta catggccgac gtgggcgcgt
     3901 cctgggccgc agccgccgcc gcctccgccg gccccttcga cagcccggtc ctgcacagcc
     3961 tgcccggccg ggccaacccg gccgcccgac accccaatct cgatatgttt gacgacttct
     4021 cagaaggcag agagtgtgtc aactgtgggg ctatgtccac cccgctctgg aggcgagatg
     4081 ggacgggtca ctatctgtgc aacgcctgcg gcctctacca caagatgaac ggcatcaacc
     4141 ggccgctcat caagcctcag cgccggctgt ccgcctcccg ccgagtgggc ctctcctgtg
     4201 ccaactgcca gaccaccacc accacgctgt ggcgccgcaa tgcggagggc gagcctgtgt
     4261 gcaatgcctg cggcctctac atgaagctcc acggggtccc caggcctctt gcaatgcgga
     4321 aagaggggat ccaaaccaga aaacggaagc ccaagaacct gaataaatct aagacaccag
     4381 cagctccttc aggcagtgag agccttcctc ccgccagcgg tgcttccagc aactccagca
     4441 acgccaccac cagcagcagc gaggagatgc gtcccatcaa gacggagcct ggcctgtcat
     4501 ctcactacgg gcacagcagc tccgtgtccc agacgttctc agtcagtgcg atgtctggcc
     4561 atgggccctc catccaccct gtcctctcgg ccctgaagct ctccccacaa ggctatgcgt
     4621 ctcccgtcag ccagtctcca cagaccagct ccaagcagga ctcttggaac agcctggtct
     4681 tggccgacag tcacggggac ataatcactg cgaagcttgg ctccggcgag ggcaggggaa
     4741 gtcttctaac atgcggggac gtggaggaaa atcccggccc actcgagatg gccgacgcag
     4801 acgagggctt tggcctggcg cacacgcctc tggagcctga cgcaaaagac ctgccctgcg
     4861 attcgaaacc cgagagcgcg ctcggggccc ccagcaagtc cccgtcgtcc ccgcaggccg
     4921 ccttcaccca gcagggcatg gagggaatca aagtgtttct ccatgaaaga gaactgtggc
     4981 taaaattcca cgaagtgggc acggaaatga tcataaccaa ggctggaagg cggatgtttc
     5041 ccagttacaa agtgaaggtg acgggcctta atcccaaaac gaagtacatt cttctcatgg
     5101 acattgtacc tgccgacgat cacagataca aattcgcaga taataaatgg tctgtgacgg
     5161 gcaaagctga gcccgccatg cctggccgcc tgtacgtgca cccagactcc cccgccaccg
     5221 gggcgcattg gatgaggcag ctcgtctcct tccagaaact caagctcacc aacaaccacc
     5281 tggacccatt tgggcatatt attctaaatt ccatgcacaa ataccagcct agattacaca
     5341 tcgtgaaagc ggatgaaaat aatggatttg gctcaaaaaa tacagcgttc tgcactcacg
     5401 tctttcctga gactgcgttt atagcagtga cttcctacca gaaccacaag atcacgcaat
     5461 taaagattga gaataatccc tttgccaaag gatttcgggg cagtgatgac atggagctgc
     5521 acagaatgtc aagaatgcaa agtaaagaat atcccgtggt ccccaggagc accgtgaggc
     5581 aaaaagtggc ctccaaccac agtcctttca gcagcgagtc tcgagctctc tccacctcat
     5641 ccaatttggg gtcccaatac cagtgtgaga atggtgtttc cggcccctcc caggacctcc
     5701 tgcctccacc caacccatac ccactgcccc aggagcatag ccaaatttac cattgtacca
     5761 agaggaaaga ggaagaatgt tccaccacag accatcccta taagaagccc tacatggaga
     5821 catcacccag tgaagaagat tccttctacc gctctagcta tccacagcag cagggcctgg
     5881 gtgcctccta caggacagag tcggcacagc ggcaagcttg catgtatgcc agctctgcgc
     5941 cccccagcga gcctgtgccc agcctagagg acatcagctg caacacgtgg ccaagcatgc
     6001 cttcctacag cagctgcacc gtcaccaccg tgcagcccat ggacaggcta ccctaccagc
     6061 acttctccgc tcacttcacc tcggggcccc tggtccctcg gctggctggc atggccaacc
     6121 atggctcccc acagctggga gagggaatgt tccagcacca gacctccgtg gcccaccagc
     6181 ctgtggtcag gcagtgtggg cctcagactg gcctgcagtc ccctggcacc cttcagcccc
     6241 ctgagttcct ctactctcat ggcgtgccaa ggactctatc ccctcatcag taccactctg
     6301 tgcacggagt tggcatggtg ccagagtgga gcgacaatag ctaagtcgag cggccgccag
     6361 cacagtggtc gacctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6541 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6601 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6661 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac
     6721 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6781 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6841 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6901 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6961 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7021 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7081 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7141 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7201 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7261 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7321 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgtc
     7381 gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     7441 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     7501 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     7561 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     7621 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     7681 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     7741 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     7801 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     7861 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     7921 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     7981 tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa
     8041 tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc
     8101 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8161 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8221 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9001 tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg
     9061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcat
//
