LOCUS       sequence-334361-       11710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC,
            Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, 
            Durocher D
  TITLE     53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin 
            mark.
  JOURNAL   Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 
            2013 Jun 12.
  PUBMED    23760478
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12318"; journalName: "Nature"; date: "2013-07-4- 4"; 
            volume: "499"; issue: "7456"; pages: "50-4"
COMMENT     Sequence Label: pcDNA5-FRT/TO-eGFP-53BP1
FEATURES             Location/Qualifiers
     source          1..11710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        168..547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        548..751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     702..722
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    753..771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    774..792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     802..826
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(923..925,926..928,929..1639)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(923..925,926..928,929..1639)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(968..989)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1229..1248)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1576..1597
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7595..7612)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7601..7825
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7958..7977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8109..8156
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8164..9186
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9316..9437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9353..9372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9407..9426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9486..9502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9486..9502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9499..9521)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9510..9526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9534..9540,9541..9558,9559..9564))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9579..9600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9717..9734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9888..10476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9968..9987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10647..11438,11439..11507))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11270..11289
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11508..11612)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11687..11706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
       61 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      121 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      181 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      241 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      301 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      361 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      421 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      481 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      541 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      601 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      661 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      721 tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag atctccctat
      781 cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
      841 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc
      901 gtttaaactt aagcttggta ccatggtgag caagggcgag gagctgttca ccggggtggt
      961 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1021 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1081 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1141 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1201 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1261 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1321 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1381 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1441 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1501 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1561 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1621 catggacgag ctgtacaagg gcgcgccaat ggaccctact ggaagtcagt tggattcaga
     1681 tttctctcag caagatactc cttgcctgat aattgaagat tctcagcctg aaagccaggt
     1741 tctagaggat gattctggtt ctcacttcag tatgctatct cgacaccttc ctaatctcca
     1801 gacgcacaaa gaaaatcctg tgttggatgt tgtgtccaat cctgaacaaa cagctggaga
     1861 agaacgagga gacggtaata gtgggttcaa tgaacatttg aaagaaaaca aggttgcaga
     1921 ccctgtggat tcttctaact tggacacatg tggttccatc agtcaggtca ttgagcagtt
     1981 acctcagcca aacaggacaa gcagtgttct gggaatgtca gtggaatctg ctcctgctgt
     2041 ggaggaagag aagggagaag agttggaaca gaaggagaaa gagaaggaag aagatacttc
     2101 aggcaatact acacattccc ttggtgctga agatactgcc tcatcacagt tgggttttgg
     2161 ggttctggaa ctctcccaga gccaggatgt tgaggaaaat actgtgccat atgaagtgga
     2221 caaagagcag ctacaatcag taaccaccaa ctctggttat accaggctgt ctgatgtgga
     2281 tgctaatact gcaattaagc atgaagaaca gtccaacgaa gatatcccca tagcagaaca
     2341 gtccagcaag gacatccctg tgacagcaca gcccagtaag gatgtacatg ttgtaaaaga
     2401 gcaaaatcca ccacctgcaa ggtcagagga catgcctttt agccccaaag catctgttgc
     2461 tgctatggaa gcaaaagaac agttgtctgc acaagaactt atggaaagtg gactgcagat
     2521 tcagaagtca ccagagcctg aggttttgtc aactcaggaa gacttgtttg accagagcaa
     2581 taaaacagta tcttctgatg gttgctctac tccttcaagg gaggaaggtg ggtgttcttt
     2641 ggcttccact cctgccacca ctctgcatct cctgcagctc tctggtcaga ggtcccttgt
     2701 tcaggacagt ctttccacga attcttcaga tcttgttgct ccttctcctg atgctttccg
     2761 atctactcct tttatcgttc ctagcagtcc cacagagcaa gaagggagac aagataagcc
     2821 aatggacacg tcagtgttat ctgaagaagg aggagagcct tttcagaaga aacttcaaag
     2881 tggtgaacca gtggagttag aaaacccccc tctcctgcct gagtccactg tatcaccaca
     2941 agcctcaaca ccaatatctc agagcacacc agtcttccct cctgggtcac ttcctatccc
     3001 atcccagcct cagttttctc atgacatttt tattccttcc ccaagtctgg aagaacaatc
     3061 aaatgatggg aagaaagatg gagatatgca tagttcatct ttgacagttg agtgttctaa
     3121 aacttcagag attgaaccaa agaattcccc tgaggatctt gggctatctt tgacagggga
     3181 ttcttgcaag ttgatgcttt ctacaagtga atatagtcag tccccaaaga tggagagctt
     3241 gagttctcac agaattgatg aagatggaga aaacacacag attgaggata cggaacccat
     3301 gtctccagtt ctcaattcta aatttgttcc tgctgaaaat gatagtatcc tgatgaatcc
     3361 agcacaggat ggtgaagtac aactgagtca gaatgatgac aaaacaaagg gagatgatac
     3421 agacaccagg gatgacatta gtattttagc cactggttgc aagggcagag aagaaacggt
     3481 agcagaagat gtttgtattg atctcacttg tgattcgggg agtcaggcag ttccgtcacc
     3541 agctactcga tctgaggcac tttctagtgt gttagatcag gaggaagcta tggaaattaa
     3601 agaacaccat ccagaggagg ggtcttcagg gtctgaggtg gaagaaatcc ctgagacacc
     3661 ttgtgaaagt caaggagagg aactcaaaga agaaaatatg gagagtgttc cgttgcacct
     3721 ttctctgact gaaactcagt cccaagggtt gtgtcttcaa aaggaaatgc caaaaaaaga
     3781 atgctcagaa gctatggaag ttgaaaccag tgtgattagt attgattccc ctcaaaagtt
     3841 ggcaatactt gaccaagaat tggaacataa ggaacaggaa gcttgggaag aagctacttc
     3901 agaggactcc agtgttgtca ttgtagatgt gaaagagcca tctcccagag ttgatgtttc
     3961 ttgtgaacct ttggagggag tggagaagtg ctcagattcc cagtcatggg aggatattgc
     4021 tccagaaata gaaccatgtg ctgagaatag attagacacc aaggaagaaa agagtgtaga
     4081 atatgaagga gatctgaaat cagggactgc agaaacagaa cctgtagagc aagattcttc
     4141 acagccttcc ttacctttag tacgcgctga cgacccgctt agacttgacc aggagttgca
     4201 gcagccccaa actcaggaga aaacaagtaa ttcattaaca gaagactcaa aaatggctaa
     4261 tgcaaagcag ctaagctcag atgcagaggc ccagaagctg gggaagccct ctgcccatgc
     4321 ctcacaaagc ttctgtgaaa gttctagtga aaccccattt catttcactt tgcctaaaga
     4381 aggtgatatc atcccaccat tgactggtgc aaccccacct cttattgggc acctaaaatt
     4441 ggagcccaag agacacagta ctcctattgg tattagcaac tatccagaaa gcaccatagc
     4501 aaccagtgat gtcatgtctg aaagcatggt ggagacccat gatcccatac ttgggagtgg
     4561 aaaaggggat tctggggctg ccccagacgt ggatgataaa ttatgtctaa gaatgaaact
     4621 ggttagtcct gagactgagg cgagtgaaga gtctttgcag ttcaacctgg aaaagcctgc
     4681 aactggtgaa agaaaaaatg gatctactgc tgttgctgag tctgttgcca gtccccagaa
     4741 gaccatgtct gtgttgagct gtatctgtga agccaggcaa gagaatgagg ctcgaagtga
     4801 ggatcccccc accacaccca tcagggggaa cttgctccac tttccaagtt ctcaaggaga
     4861 agaggagaaa gaaaaattgg agggtgacca tacaatcagg cagagtcaac agcctatgaa
     4921 gcccattagt cctgtcaagg accctgtttc tcctgcttcc cagaagatgg tcatacaagg
     4981 gccatccagt cctcaaggag aggcaatggt gacagatgtg ctagaagacc agaaagaagg
     5041 acggagtact aataaggaaa atcctagtaa ggccttgatt gaaaggccca gccaaaataa
     5101 cataggaatc caaaccatgg agtgttcctt gagggtccca gaaactgttt cagcagcaac
     5161 ccagactata aagaatgtgt gtgagcaggg gaccagtaca gtggaccaga actttggaaa
     5221 gcaagatgcc acagttcaga ctgagagggg gagtggtgag aaaccagtca gtgctcctgg
     5281 ggatgataca gagtcgctcc atagccaggg agaagaagag tttgatatgc ctcagcctcc
     5341 acatggccat gtcttacatc gtcacatgag aacaatccgg gaagtacgca cacttgtcac
     5401 tcgtgtcatt acagatgtgt attatgtgga tggaacagaa gtagaaagaa aagtaactga
     5461 ggagactgaa gagccaattg tagagtgtca ggagtgtgaa actgaagttt ccccttcaca
     5521 gactgggggc tcctcaggtg acctggggga tatcagctcc ttctcctcca aggcatccag
     5581 cttacaccgc acatcaagtg ggacaagtct ctcagctatg cacagcagtg gaagctcagg
     5641 gaaaggagcc ggaccactca gagggaaaac cagcgggaca gaacccgcag attttgcctt
     5701 acccagctcc cgaggaggcc caggaaaact gagtcctaga aaaggggtca gtcagacagg
     5761 gacgccagtg tgtgaggagg atggtgatgc aggccttggc atcagacagg gagggaaggc
     5821 tccagtcacg cctcgtgggc gtgggcgaag gggccgccca ccttctcgga ccactggaac
     5881 cagagaaaca gctgtgcctg gccccttggg catagaggac atttcaccta acttgtcacc
     5941 agatgataaa tccttcagcc gtgtcgtgcc ccgagtgcca gactccacca gacgaacaga
     6001 tgtgggtgct ggtgctttgc gtcgtagtga ctctccagaa attcctttcc aggctgctgc
     6061 tggcccttct gatggcttag atgcctcctc tccaggaaat agctttgtag ggctccgtgt
     6121 tgtagccaag tggtcatcca atggctactt ttactctggg aaaatcacac gagatgtcgg
     6181 agctgggaag tataaattgc tctttgatga tgggtacgaa tgtgatgtgt tgggcaaaga
     6241 cattctgtta tgtgacccca tcccgctgga cactgaagtg acggccctct cggaggatga
     6301 gtatttcagt gcaggagtgg tgaaaggaca taggaaggag tctggggaac tgtactacag
     6361 cattgaaaaa gaaggccaaa gaaagtggta taagcgaatg gctgtcatcc tgtccttgga
     6421 gcaaggaaac agactgagag agcagtatgg gcttggcccc tatgaagcag taacacctct
     6481 tacaaaggca gcagatatca gcttagacaa tttggtggaa gggaagcgga aacggcgcag
     6541 taacgtcagc tccccagcca cccctactgc ctccagtagc agcagcacaa cccctacccg
     6601 aaagatcaca gaaagtcctc gtgcctccat gggagttctc tcaggcaaaa gaaaacttat
     6661 cacttctgaa gaggaacggt cccctgccaa gcgaggtcgc aagtctgcca cagtaaaacc
     6721 tggtgcagta ggggcaggag agtttgtgag cccctgtgag agtggagaca acaccggtga
     6781 accctctgcc ctggaagagc agagagggcc tttgcctctc aacaagacct tgtttctggg
     6841 ctacgcattt ctccttacca tggccacaac cagtgacaag ttggccagcc gctccaaact
     6901 gccagatggt cctacaggaa gcagtgaaga agaggaggaa tttttggaaa ttcctccttt
     6961 caacaagcag tatacagaat cccagcttcg agcaggagct ggctatatcc ttgaagattt
     7021 caatgaagcc cagtgtaaca cagcttacca gtgtcttcta attgcggatc agcattgtcg
     7081 aacccggaag tacttcctgt gccttgccag tgggattcct tgtgtgtctc atgtctgggt
     7141 ccatgatagt tgccatgcca accagctcca gaactaccgt aattatctgt tgccagctgg
     7201 gtacagcctt gaggagcaaa gaattctgga ctggcaaccc cgtgaaaatc ctttccagaa
     7261 tctgaaggta ctcttggtat cagaccaaca gcagaacttc ctggagctct ggtctgagat
     7321 cctcatgact ggtggtgcag cctctgtgaa gcagcaccat tcaagtgccc ataacaaaga
     7381 tattgcttta ggggtatttg atgtggtggt gacggacccc tcatgcccag cctcggtgct
     7441 gaagtgtgct gaagcattgc agctgcctgt ggtgtcacaa gagtgggtga tccagtgcct
     7501 cattgttggg gagagaattg gattcaagca gcatccaaaa tataaacacg attatgtttc
     7561 tcactaaagg ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     7621 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7681 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7741 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7801 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     7861 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7921 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7981 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8041 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8101 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg
     8161 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     8221 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     8281 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     8341 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     8401 ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     8461 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     8521 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     8581 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     8641 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     8701 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     8761 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     8821 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     8881 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     8941 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     9001 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     9061 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     9121 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     9181 gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     9241 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     9301 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9361 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9421 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     9481 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9541 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10321 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    11281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    11341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11461 gccgcaaaaa agggaataag ggcgacgcgg aaatgttgaa tactcatact cttccttttt
    11521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    11641 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
    11701 atgccgcata
//
