LOCUS       sequence-334364-        3022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/601
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(473..1264,1265..1333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1096..1115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1334..1438)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1561..1583)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1683..1702
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1896..1918
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1910..1927
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1911..1927
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1934..1953
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1934..1952
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2299..2317)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2300..2317)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2335..2351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2335..2351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2348..2370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2359..2375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2383..2389,2390..2407,2408..2413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2428..2449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2566..2583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2737..3022,1..302))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2817..2836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
       61 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      121 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      181 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatcggcaaa caaaccaccg
      241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      781 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
      961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1081 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1441 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1501 ataaaaatag cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     1561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     1621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     1681 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     1741 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     1801 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     1861 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     1921 ggccagtgaa ttgtaatacg actcactata gggcgaattc gagctcggta cccggggatc
     1981 ctctagagtg ggagctcgga acactatccg actggcaccg gcaaggtcgc tgttcaatac
     2041 atgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     2101 ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     2161 attgagcggc ctcggcaccg ggattctcca gggcggccgc gtatagggtc catcacataa
     2221 gggatgaact cggtgtgaag aatcatgctt tccttggtca ttaggatccc ggacctgcag
     2281 gcatgcaagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
     2341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3001 ccccgttcag cccgaccgct gc
//
