LOCUS       sequence-33437-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, 
            Wolberger C
  TITLE     Structural insights into the assembly and function of the SAGA 
            deubiquitinating module.
  JOURNAL   Science. 2010 May 21. 328(5981):1025-9.
  PUBMED    20395473
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2010 May 21. 328(5981):1025-9."
COMMENT     Sequence Label: Sus1/PET32a
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(354..374)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(384..398)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(414..458)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(465..482)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(492..509)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(531..857)
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     RBS             865..887
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    902..926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccttcgggct tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gctcattgtg tatctacaat ctcttcaaga aattccctta tttgctttaa aacggtttcc
      121 cttgttgaat ccgataccat ttctaatgct ttgggttcta cggtggataa aatttgggta
      181 aagttcgtag attcatttat gttcatctct gatttggtca aatctttgac tttatccacc
      241 caaccttctt gaagtagtct ggcttttagt tcgtttgaaa ttagttcata gtttcctgat
      301 tctacaagat attgttgtat ttgactcttt aattgcgcag tatccatagt cattccctga
      361 aaatacaggt tttcagccat ggccttgtcg tcgtcgtcgg tacccagatc tgggctgtcc
      421 atgtgctggc gttcgaattt agcagcagcg gtttctttca taccagaacc gcgtggcacc
      481 agaccagaag aatgatgatg atgatggtgc atatggccag aaccagaacc ggccaggtta
      541 gcgtcgagga actctttcaa ctgaccttta gacagtgcac ccactttggt tgccgccact
      601 tcaccgtttt tgaacagcag cagagtcggg ataccacgga tgccatattt cggcgcagtg
      661 ccagggtttt gatcgatgtt cagttttgca acggtcagtt tgccctgata ttcgtcagcg
      721 atttcatcca gaatcggggc gatcattttg cacggaccgc accactctgc ccagaaatcg
      781 acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa aactgtcgtc agtcaggtga
      841 ataattttat cgctcatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      901 gggaattgtt atccgctcac aattcccaag gggggagtcg at
//
