LOCUS       sequence-33438-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, 
            Wolberger C
  TITLE     Structural insights into the assembly and function of the SAGA 
            deubiquitinating module.
  JOURNAL   Science. 2010 May 21. 328(5981):1025-9.
  PUBMED    20395473
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2010 May 21. 328(5981):1025-9."
COMMENT     Sequence Label: Sgf73_Sgf11/PCDFDuet
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        122..140
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    141..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        580..598
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    599..623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
       61 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa
      121 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt
      181 ttgtttaact ttaataagga gatataccat ggctatgacc gaagaaacta ttactataga
      241 ttctatttca aatgggatac tgaataacct gttaaccaca ttgatccagg acattgtagc
      301 tcgggaaacc actcaacaac aattgctgaa gacaagatat ccggatcttc gcagttatta
      361 tttcgacccg aacggatctc tcgatattaa tggactacag aagcaacaag agtcctctca
      421 gtatattcac tgtgagaatt gtggcaggga tgtgtccgca aacagactag cagctcattt
      481 acagagatgt ttgagtaggg gtgctagacg ttgagcggcc gcataatgct taagtcgaac
      541 agaaagtaat cgtattgtac acggccgcat aatcgaaatt aatacgactc actatagggg
      601 aattgtgagc ggataacaat tccccatctt agtatattag ttaagtataa gaaggagata
      661 tacatatgag gtcaggcgat gcagagatta aaggaattaa gcccaaagtt attgaggaat
      721 attctttatc tcagggaagt ggtccttcca acgattcatg gaaaagttta atgtcgtccg
      781 cgaaggacac tcctttgcag tatgatcaca tgaatcgcga aagtct
//
