LOCUS       sequence-334382-        9383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with
            NLS-mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM
  TITLE     Budding epithelial morphogenesis driven by cell-matrix versus 
            cell-cell adhesion.
  JOURNAL   Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 
            10.1016/j.cell.2021.05.015. Epub 2021 Jun 15.
  PUBMED    34133940
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.05.015"; journalName: "Cell"; date: "2021-07-8-
            8"; volume: "184"; issue: "14"; pages: "3702-3716.e30"
COMMENT     Sequence Label: 
            pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..9383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..44)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        29..358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      209..344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     271..290
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        406..453
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             472..846
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    976..1097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1013..1032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1067..1086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1146..1162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1146..1162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1159..1181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1170..1186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1194..1200,1201..1218,1219..1224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1239..1260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1377..1394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1548..2136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1628..1647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2307..3098,3099..3167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2930..2949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3168..3272)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3347..3366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3538..3917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3919..4117
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4072..4092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4135..4315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4362..4487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4980..5213
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5398..5442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5740..5857
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5908..6156
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5908..5928
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6079..6098
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6178..6253
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6315..6526
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6554..6601
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6602..7294
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(6732..6750)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7000..7019
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7304..7360
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7361..7759
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7775..8363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7828..7848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8246..8257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8435..8668
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8694..8711)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8700..8924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8970..9383,1..15)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9057..9076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9267..9288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
       61 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
      301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      361 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
      421 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
      481 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
      541 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
      601 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
      661 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
      721 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
      781 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
      841 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
      901 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
      961 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1021 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1081 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     1141 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1201 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1261 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1321 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1981 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3301 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     3361 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     3421 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     3481 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     3541 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3601 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3781 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3841 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3901 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4021 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4081 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     4141 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4201 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4261 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     4321 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     4381 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     4441 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     4501 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     4561 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     4621 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     4681 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     4741 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     4801 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     4861 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     4921 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     4981 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     5041 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     5101 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     5161 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     5221 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     5281 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     5341 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     5401 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     5461 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     5521 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     5581 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     5641 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     5701 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     5761 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     5821 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     5881 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     5941 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     6001 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     6061 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     6121 ttggctttat atatcttgtg gaaaggacga aacaccgccg agaggctgcg gctccaagtt
     6181 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     6241 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     6301 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6361 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     6421 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6481 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag
     6541 cgctgccacc atgaagcgac ctgccgccac aaagaaggct ggacaggcta agaagaagaa
     6601 agtgagcaag ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg
     6661 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6721 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat
     6781 cctgtcccct cagttcatgt acggctccag ggccttcatc aagcaccccg ccgacatccc
     6841 cgactactat aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6901 ggacggcggc gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta
     6961 caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac
     7021 aatgggctgg gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga
     7081 cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac
     7141 ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga
     7201 catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg
     7261 ccactccacc ggcggcatgg acgagctgta caagggatcc ggcgcaacaa acttctctct
     7321 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atggccaagc ctttgtctca
     7381 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     7441 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     7501 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     7561 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     7621 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     7681 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     7741 ttatgtgtgg gagggctaaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     7801 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7861 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7921 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7981 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8041 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8101 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8161 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     8221 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8281 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8341 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     8401 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8461 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     8521 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8581 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8641 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     8701 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8761 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8821 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8881 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     8941 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     9001 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9061 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9121 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9181 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9241 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9301 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9361 aaaaaatgag ctgatttaac aaa
//
